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Molecular epidemiology of lumpy skin disease outbreak in Odisha, India

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Abstract

Lumpy skin disease virus (LSDV) is the causative agent of lumpy skin disease (LSD) which is a member of Capripoxvirus. It is an economically critical transboundary disease affecting cattle. This study records an LSD outbreak in Ganjam district of Odisha, India during August 2020. The epidemiological data were analysed and LSDV was genetically characterized. Out of the 452 animals clinically examined (59 farms), 63 animals were clinically affected with LSD, with a total morbidity rate of 13.93%. The morbidity rates in the surveyed villages (n = 10) varied from 5.55 to 21.62%. The multivariable logistic regression analysis showed that grazing of animals (P = 0.013; OR: 2.04; 95% CI: 1.16–3.57) and age of cows > 3 years old (P = 0.001; OR: 2.90; 95% CI: 1.65- 5.07) were potential risk factors for the presence of LSD. Out of the 53 clinically suspected animals’ samples, 18 samples (33.96%) were found positive for both the P32 and F genes of Capripoxvirus by PCR. Phylogenetic analysis of the P32 gene of LSDV (MW147486) showed 100% similarity with other isolates from India, Bangladesh, Egypt and Saudi Arabia. Additionally, phylogenetic analysis of the F gene of LSDV (MW147485) revealed a similarity of 97.99%, with Odisha India (MT074110) isolate and located in the same cluster with other Indian isolates.

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Authors and Affiliations

  1. Department of Clinical Medicine, College of Veterinary Science and Animal Husbandry, Odisha University of Agriculture and Technology, Bhubaneshwar 751003, Odisha, India

    Rajesh Kumar Sethi, Santosh Kumar Senapati & Manoranjan Das

  2. Internal Medicine, Infectious and Fish Diseases Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt

    Ahmed Magdy Selim

  3. Department of Veterinary Pathology, College of Veterinary Science and Animal Husbandry, Odisha University of Agriculture and Technology, Bhubaneshwar 751003, Odisha, India

    Aditya Prasad Acharya

  4. Department of Animal Breeding and Genetics, College of Veterinary Science and Animal Husbandry, Odisha University of Agriculture and Technology, Bhubaneshwar 751003, Odisha, India

    Chinmoy Mishra

  5. Animal Medicine Department, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, Egypt

    Yamen Mohammed Hegazy

  6. Department of Veterinary Gyanecology and Obstetrics, College of Veterinary Science and Animal Husbandry, Odisha University of Agriculture and Technology, Bhubaneshwar 751003, Odisha, India

    Shuvranshu Shekhar Biswal

Authors
  1. Rajesh Kumar Sethi
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  2. Santosh Kumar Senapati
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  3. Ahmed Magdy Selim
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  4. Aditya Prasad Acharya
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  5. Chinmoy Mishra
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  6. Manoranjan Das
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  7. Yamen Mohammed Hegazy
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  8. Shuvranshu Shekhar Biswal
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Contributions

Santosh Senapati and Manoranjan das conceived and planned the study; Ahmed Selim and Rajesh Sethi collaborated in the writing and revision of the manuscript; Rajesh Sethi and Aditya Prasad Acharya conducted laboratory testing; Ahmed Selim and Chinmoy Mishra collaborated is sequencing of genes and phylogenetic analysis. Santosh Senapati and Shuvranshu Biswal revised the manuscript. Yamen Mohammed Hegazy performed the statistical analysis. All authors' read and endorsed the final manuscript.

Corresponding author

Correspondence to Santosh Kumar Senapati.

Ethics declarations

Ethics approval

Oral permission and written was taken from owners of the farms before collection of samples. Additionally, samples collection was performed by professional field veterinarians.

Consent to participate

Oral and written permission was taken from owners of the farms before collection of samples. There is no specific law in India which requires permission from the ethics committee for collection of samples for clinical diagnosis. Additionally, samples collection was performed by professional field veterinarians.

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We know of no conflicts of interest associated with this publication. As Corresponding Author, I confirm that the manuscript has been read and approved for submission by all the named authors.

Conflict of interest

The authors of this study stated that they have no conflicts of interest.

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Sethi, R.K., Senapati, S.K., Selim, A.M. et al. Molecular epidemiology of lumpy skin disease outbreak in Odisha, India. Vet Res Commun 46, 711–717 (2022). https://doi.org/10.1007/s11259-022-09886-8

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  • DOI: https://doi.org/10.1007/s11259-022-09886-8

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