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Age-specific plasma biochemistry reference ranges in <1 year old dogs in Japan

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Abstract

The purpose of this study was to investigate and propose possible reference intervals of plasma biochemical analytes in young dogs (<12 months old) in Japan, using 896 canine plasma samples, collected from an array of veterinary clinics throughout the greater Tokyo metropolis area in Japan. The following biochemical parameters were assessed: albumin (ALB), alanine aminotransferase (ALT), alkaline phosphatase (ALP), amylase, aspartate aminotransferase (AST), blood urea nitrogen (BUN), creatinine (CRE), glucose, lipase, total cholesterol (T-Cho), and total protein (TP) were evaluated. Multivariate linear regression analysis indicated that partitioning according to age or gender may be necessary for some plasma analytes. Age appeared to significantly affect ALB, ALT, ALP, BUN, Glucose, Lipase, and Total Protein (P = <0.001, <0.001, <0.001, 0.013, <0.001, 0.025, and <0.001, respectively). On the other hand, gender significantly influenced ALP, Amylase, Lipase, and T-Cho levels (P = 0.017, <0.001, <0.001, and <0.001, respectively) whereas it may be borderline significant with ALT (P = 0.072).

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Abbreviations

ALT:

alanine aminotransferase

ALB:

albumin

ALP:

alkaline phosphatase

AST:

aspartate aminotransferase

BUN:

blood urea nitrogen

CRE:

creatinine

T-CHO:

total cholesterol

TP:

total protein

References

  • American Diabetes Association (2007) Standards of medical care in diabetes—2007. Diab Care 30:S4–S41

    Article  Google Scholar 

  • Ceriotti F (2007) Prerequisites for use of common reference intervals. Clin Biochem Rev 28:115–121

    PubMed  Google Scholar 

  • Clinical and Laboratory Standards Institute (2008) Defining, establishing, and verifying reference intervals in the clinical laboratory; approved guideline, 3rd edn. CLSI document C28-A3. Clinical and Laboratory Standards Institute (CLSI), Wayne

    Google Scholar 

  • Comazzi S, Pieralisi C, Bertazzolo W (2004) Haematological and biochemical abnormalities in canine blood: frequency and associations in 1022 samples. J Small Anim Pract 45:343–349

    Article  PubMed  CAS  Google Scholar 

  • Dunlop MM, Sanchez-Vazquez MJ, Freeman KP, Gibson G, Sacchini F, Lewis F (2011) Determination of serum biochemistry reference intervals in a large sample of adult greyhounds. J Small Anim Pract 52:4–10

    Article  PubMed  CAS  Google Scholar 

  • Geffre A, Friedrichs K, Harr K, Concordet D, Trumel C, Braun J-P (2009) Reference intervals: a review. Vet Clin Pathol 38:288–298

    Article  PubMed  Google Scholar 

  • Ikeuchi J, Yoshizaki T, Hirata M (1991) Plasma biochemistry values of young beagle dogs. J Toxicol Sci 16:49–59

    PubMed  CAS  Google Scholar 

  • Katayev A, Balciza C, Seccombe DW (2010) Establishing reference intervals for clinical laboratory test results: is there a better way? Am J Clin Pathol 133:180–186

    Article  PubMed  CAS  Google Scholar 

  • Mori N, Lee P, Muranaka S, Sagara F, Takemitsu H, Nishiyama Y, Yamamoto I, Yagishita M, Arai T (2010) Predisposition for primary hyperlipidemia in Miniature Schnauzers and Shetland sheepdogs as compared to other canine breeds. Res Vet Sci 88:394–399

    Article  PubMed  CAS  Google Scholar 

  • Nielsen L, Kjelgaard-Hansen M, Jensen AL, Kristensen AT (2010) Breed-specific variation of hematologic and biochemical analytes in healthy adult Bernese Mountain dogs. Vet Clin Pathol 39:20–28

    Article  PubMed  Google Scholar 

  • Shapiro SS, Wilk MB (1965) An analysis of variance test for normality (complete samples). Biometrika 52:591–611

    Google Scholar 

  • Sharkey L, Gjevre K, Hegstad-Davies R, Torres S, Muñoz-Zanzi C (2009) Breed-associated variability in serum biochemical analytes in four large-breed dogs. Vet Clin Pathol 38:375–380

    Article  PubMed  Google Scholar 

  • Solberg HE (1983) The theory of reference values Part 5. Statistical treatment of collected reference values. Determination of reference limits. J Clin Chem Clin Biochem 21:749–760

    PubMed  CAS  Google Scholar 

  • Willard MD, Twedt DC (1994) Gastrointestinal, pancreatic and hepatic disorders. In: Willard MD, Tvedten H, Turnwald GH (eds) Small animal clinical diagnosis by laboratory methods, 2nd edn. W.B Saunders, Philadelphia, pp 179–218

    Google Scholar 

Download references

Acknowledgements

This work was supported in part by the MEXT 2008 Strategic Research Base Development Program for Private Universities.

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Authors and Affiliations

  1. School of Veterinary Nursing & Technology, Nippon Veterinary and Life Science University, 1-7-1 Kyonancho, Musashino, Tokyo, 180-8602, Japan

    Toshinori Sako, Akihiro Mori, Ayano Igarashi, Hitomi Oda, Kaori Saeki, Yohei Miki, Miyuki Kurishima, Kana Mimura, Satoshi Nozawa & Katsumi Ishioka

  2. School of Veterinary Medicine, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, 1-7-1 Kyonancho, Musashino, Tokyo, 180-8602, Japan

    Peter Lee & Toshiro Arai

  3. Monolis Inc., Tokyo, Japan

    Yukiko Itabashi & Yoshio Kiyosawa

Authors
  1. Toshinori Sako
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  2. Akihiro Mori
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  3. Peter Lee
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  4. Ayano Igarashi
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  5. Hitomi Oda
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  6. Kaori Saeki
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  7. Yohei Miki
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  8. Miyuki Kurishima
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  9. Kana Mimura
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  10. Satoshi Nozawa
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  11. Yukiko Itabashi
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  12. Yoshio Kiyosawa
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  13. Katsumi Ishioka
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  14. Toshiro Arai
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Corresponding author

Correspondence to Akihiro Mori.

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Sako, T., Mori, A., Lee, P. et al. Age-specific plasma biochemistry reference ranges in <1 year old dogs in Japan. Vet Res Commun 35, 201–209 (2011). https://doi.org/10.1007/s11259-011-9463-0

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  • DOI: https://doi.org/10.1007/s11259-011-9463-0

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