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The detection of “Candidatus Mycoplasma haemobos” in cattle and buffalo in China

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Abstract

“Candidatus Mycoplasma haemobos” is a hemoplasma species found in cattle and has been recently reported in Switzerland and Japan. In this study, “Candidatus Mycoplasma haemobos” was shown to occur in cattle and buffalo in tropical China by PCR amplification and sequence analysis of the 16S rRNA gene from blood samples. Based on the 16S rDNA sequence, a specific PCR assay was developed. Occurrence of “Candidatus Mycoplasma haemobos” in cattle and buffalo in Guangxi, China, was determined by examining 25 buffalo blood samples, 12 yellow cattle blood samples and 42 dairy cow blood samples. The results showed that 32% (8/25) of buffalo, 41.7% (5/12) of yellow cattle, and 14.3% (6/42) of dairy cows were positive for “Candidatus Mycoplasma haemobos”, respectively. Direct sequencing of representative PCR products confirmed that the amplified partial 16S rDNA sequence represented “Candidatus Mycoplasma haemobos”. This is the first report of “Candidatus Mycoplasma haemobos” in buffalo, yellow cattle, and dairy cows in China.

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Acknowledgements

Project support was provided by the Program for Changjiang Scholars and Innovative Research Team in University (grant no. IRT0723) and the State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences. The authors are grateful to Dr. Alasdair Nisbet, Parasitology Division, Moredun Research Institute, United Kingdom for improving the quality of the manuscript.

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Authors and Affiliations

  1. Guangxi Veterinary Research Institute, 51 Youai Bei Road, Nanning, Guangxi Zhuang Nationality Autonomous Region, 530001, People’s Republic of China

    Q. L. Su

  2. State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, CAAS, Lanzhou, Gansu Province, 730046, People’s Republic of China

    H. Q. Song & Xing Quan Zhu

  3. College of Veterinary Medicine, South China Agricultural University, 483 Wushan Street, Tianhe District, Guangzhou, Guangdong Province, 510642, People’s Republic of China

    H. Q. Song, R. Q. Lin, Z. G. Yuan, G. H. Zhao & Xing Quan Zhu

  4. College of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan Province, 650201, People’s Republic of China

    J. F. Yang

  5. College of Animal Science and Technology, Guangxi University, Nanning, Guangxi Zhuang Nationality Autonomous Region, 530004, People’s Republic of China

    Q. L. Su & W. Y. Huang

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  1. Q. L. Su
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  2. H. Q. Song
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  3. R. Q. Lin
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  4. Z. G. Yuan
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  5. J. F. Yang
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  6. G. H. Zhao
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  7. W. Y. Huang
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  8. Xing Quan Zhu
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Correspondence to Xing Quan Zhu.

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Q. L. Su and H. Q. Song contributed equally to this work.

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Su, Q.L., Song, H.Q., Lin, R.Q. et al. The detection of “Candidatus Mycoplasma haemobos” in cattle and buffalo in China. Trop Anim Health Prod 42, 1805–1808 (2010). https://doi.org/10.1007/s11250-010-9640-0

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  • DOI: https://doi.org/10.1007/s11250-010-9640-0

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