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Phototropic bending of intact and wounded potato shoots

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Abstract

Intact and wounded shoots of in vitro cultured potato were investigated in situ to determine how their phototropic (PT) bending capacity was affected by water availability and exogenous auxin supplementation. Wounding strongly decreased PT bending but it recovered upon supplementation of water, auxin or both. Intact shoots required neither water nor auxin, while shoot segments required both. Shoot cuttings required only water, while, like shoot segments, decapitated shoots had a strong requirement for auxin. Water supplementation was beneficial in all treatments and PT bending was not affected in cultures that were submerged in water for a short period. Sucrose and inorganic salts present in the medium strongly affected PT bending capacity of cultures, favouring combinations with lower concentrations of both. Sucrose alone strongly promoted PT bending up to a concentration of 5%. Osmotic shock induced by the addition of small volumes of highly concentrated carbohydrate solutions (sucrose, glucose or sorbitol) induced a rapid but transient decline in PT bending capacity. These results indicate that water availability is a major factor that affects PT bending in potato plantlets.

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Acknowledgements

Research was sponsored by the Serbian Ministry of Education, Science and Technological Development, Project No. 173015 and by a grant from Swiss National Science Foundation, SCOPES project 152221.

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Authors and Affiliations

  1. Institute for Biological Research, University of Belgrade, Bulevar Despota Stefana 142, Belgrade, 11000, Serbia

    Dragan Vinterhalter & Branka Vinterhalter

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  1. Dragan Vinterhalter
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  2. Branka Vinterhalter
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Correspondence to Dragan Vinterhalter.

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Communicated by Richard E. Veilleux.

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Vinterhalter, D., Vinterhalter, B. Phototropic bending of intact and wounded potato shoots. Plant Cell Tiss Organ Cult 130, 393–404 (2017). https://doi.org/10.1007/s11240-017-1235-2

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