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Agrobacterium-mediated transformed transgenic triploid bermudagrass (Cynodon dactylon × C. transvaalensis) plants are highly resistant to the glufosinate herbicide Liberty

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Abstract

Glufosinate resistance gene isolated from Streptomyces hygromicinroscopicus (bar) that confers the resistance of herbicide Liberty, a broad-spectrum grass and broadleaf contact herbicide widely used for weed control, was introduced into triploid bermudagrass by Agrobacterium-mediated transformation. Embryogenic calluses derived from stolonous nodal segment were co-cultured with the disarmed strain EHA105 harboring the binary vector pBG1300H containing the bar gene under the control of adh-1 promoter. A total of 18 independent transgenic lines were obtained. The integration of bar gene into plant genome was confirmed by the GUS histochemical staining assay, PCR amplification, and Southern blotting. Herbicide bioassay indicated that the bar-expressing transgenic plants exhibited greater herbicide resistance than the wild type and the non-transformed tissue culture-derived plants.

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Abbreviations

2,4-d:

2,4-dichlorophenoxyacetic acid

ABA:

abscisic acid

AS:

acetosyringone

BA:

6-benzyladenine

CH:

casein hydrolysate

GUS:

glucuronidase

NAA:

naphthyl acetic acid

PCR:

polymerase chain reaction

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Authors and Affiliations

  1. Mutational and Molecular Genetics Lab, Institute of Nuclear Agricultural Sciences, Zhejiang University, Hangzhou, 310029, P.R.China

    Fanrong Hu, Lei Zhang, Xueyan Wang, Jie Ding & Dianxing Wu

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  1. Fanrong Hu
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  2. Lei Zhang
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  3. Xueyan Wang
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  4. Jie Ding
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  5. Dianxing Wu
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Correspondence to Dianxing Wu.

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Hu, F., Zhang, L., Wang, X. et al. Agrobacterium-mediated transformed transgenic triploid bermudagrass (Cynodon dactylon × C. transvaalensis) plants are highly resistant to the glufosinate herbicide Liberty. Plant Cell Tiss Organ Cult 83, 13–19 (2005). https://doi.org/10.1007/s11240-005-3705-1

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  • DOI: https://doi.org/10.1007/s11240-005-3705-1

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