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Characterization and Anti-Aging Activity of Polysaccharides from Akebia trifoliata Fruit Separated by an Aqueous Two-Phase System

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Abstract

Bioactive polysaccharides have numerous pharmacological effects that are beneficial to human health. Akebia trifoliata (Thunb.) Koidz. has great development prospects as a food resource with medicinal value. The polysaccharides (ATFP) were extracted from A. trifoliata fruit by an aqueous two-phase system. ATFP-3, purified with DEAE-52 and Sephadex G-200 from ATFP, was mainly composed of glucose (47.55%) and galactose (20.39%). Its hydroxyl radical scavenging rate was 89.30% at 1.60 mg/mL and its IC50 was 0.29 mg/mL. ATFP-3 significantly enhanced the survival rate of Caenorhabditis elegans under thermal or oxidative stress. Furthermore, ATFP-3 could prolong the lifespan of C. elegans and improve the activities of the antioxidant enzyme, while also decrease the accumulation of lipofuscin and the level of malondialdehyde (MDA) in aging worms. Thus, ATFP-3 has application potential in health benefits for humans.

Highlights

  1. 1.

    A polysaccharide (ATFP-3) was isolated from Akebia trifoliata fruit by an aqueous two-phase system.

  2. 2.

    ATFP-3 exhibited strong activities against hydroxyl free radicals in vitro.

  3. 3.

    ATFP-3 reduced oxidative damage and prolonged the lifespan of Caenorhabditis elegans.

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Data Availability

The datasets used in the current study are available from the corresponding author on reasonable request.

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Funding

This work was supported by the Department of Science and Technology of Sichuan Province [2020JDRC0084], the Science and Technology Innovation and Entrepreneurship Project of Ya’an, and Sichuan Agricultural University.

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Authors and Affiliations

  1. College of Life Science, Sichuan Agricultural University, Ya’an, 625014, People’s Republic of China

    Zhonghao Zhang, Tao Gao, Ningning Yan, Zhihao Duan, Zizhong Tang, Lijun Zhou, Tao Chen, Shiling Feng, Chunbang Ding & Ming Yuan

  2. College of Resources, Sichuan Agricultural University, Chengdu, China

    Shu Yuan

Authors
  1. Zhonghao Zhang
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  2. Tao Gao
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  3. Ningning Yan
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  11. Ming Yuan
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Contributions

All authors contributed to the study's conception and design. Conceptualization, Methodology, Investigation, Formal analysis, Data curation, and Writing—original draft were performed by Zhonghao Zhang. Methodology was done by Ningning Yan, Tao Gao, and Zhihao Duan. Writing—review & editing were done by Zizhong Tang, Lijun Zhou, Tao Chen, and Shiling Feng. Supervision was done by Chunbang Ding. Validation was done by Shu Yuan. Conceptualization, supervision, resources, funding acquisition, project administration, and Writing—review & editing were done by Ming Yuan. All authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Ming Yuan.

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Zhang, Z., Gao, T., Yan, N. et al. Characterization and Anti-Aging Activity of Polysaccharides from Akebia trifoliata Fruit Separated by an Aqueous Two-Phase System. Plant Foods Hum Nutr 78, 154–159 (2023). https://doi.org/10.1007/s11130-022-01031-9

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