Abstract
Time-resolved (TR) infrared (IR) spectroscopy in the nanosecond to second timescale has been extensively used, in the last 30 years, in the study of photosynthetic systems. Interesting results have also been obtained at lower time resolution (minutes or even hours). In this review, we first describe the used techniques—dispersive IR, laser diode IR, rapid-scan Fourier transform (FT)IR, step-scan FTIR—underlying the advantages and disadvantages of each of them. Then, the main TR-IR results obtained so far in the investigation of photosynthetic reactions (in reaction centers, in light-harvesting systems, but also in entire membranes or even in living organisms) are presented. Finally, after the general conclusions, the perspectives in the field of TR-IR applied to photosynthesis are described.
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Notes
In the last 15 years, at least two independent research groups have tried (or are trying) to apply step-scan FTIR-DS to PSII. To our knowledge, no data have been published on the subject. A PhD thesis was totally devoted to the subject (Süss 2011).
So far, to our knowledge, no biophysical applications of mid-IR-TR frequency-comb spectroscopy have been reported, so we are a bit reluctant to include this new, revolutionary and extremely powerful technique in the perspectives. However, its application to photo-induced reactions has already been reported (Fleischer et al. 2014).
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The authors thank Prof. G. Venturoli and Dr. M. Malferrari for stimulating discussion. A.M. thanks COST Action TD1102- Phototech for giving him the possibility of fruitful discussions with several European scientists working in photosynthesis research.
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Mezzetti, A., Leibl, W. Time-resolved infrared spectroscopy in the study of photosynthetic systems. Photosynth Res 131, 121–144 (2017). https://doi.org/10.1007/s11120-016-0305-3
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DOI: https://doi.org/10.1007/s11120-016-0305-3