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Drought-induced changes in photosynthetic apparatus and antioxidant components of wheat (Triticum durum Desf.) varieties

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Abstract

Water deficit is a key factor influencing the yield and quality of crops. In the present study, the photosynthetic responses by means of chlorophyll fluorescence of chloroplasts, thylakoid membrane proteins, and antioxidant components were analyzed in wheat (Triticum durum Desf.) plants differing in their tolerance to drought. Two durum winter wheat varieties, Barakatli 95 (drought tolerant) and Garagylchyg 2 (drought sensitive) were grown under field well-watered and drought conditions. It was found that contents of the PS I core (CPI) with Mr of 123 kD and apoprotein P700 with Mr of 63 kD were relatively higher in Barakatli 95 variety under drought stress compared with the control plants. Synthesis of α- and β-subunits of CF1 ATP-synthase complex with Mr of 55 and 53.5 kD also slightly increased in the tolerant Barakatli 95 and decreased in the drought sensitive variety Garagylchyg 2. A decrease in the intensity of 30 kD band and a significant increase were found in the content of the 25–16 kD region in Garagylchyg 2 variety. The synthesis of 60 kD and content of low molecular mass polypeptides (21.5 and 12 kD) were increased in the tolerant genotype Barakatli 95. The intensity of peaks at 687, 695, and 742 nm considerably increases in the fluorescence spectra (77 K) of chloroplasts isolated from the sensitive variety Garagylchyg 2, and there is a stimulation of the ratio of fluorescence band intensity F687/F740. At the same time, higher level of glycine betaine was found in the drought tolerant variety compared with the control one throughout the different periods of growth.

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Abbreviations

ROS:

Reactive oxygen species

AsA:

Ascorbic acid

GB:

Glycine betaine

PS I:

Photosystem I

PS II:

Photosystem II

LHC:

Light-harvesting chlorophyll a/b-protein complex

Chl:

Chlorophyll

SDS:

Sodium dodecyl sulfate

F:

Fluorescence

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Acknowledgments

This work was supported by the Science Development Foundation under the President of the Republic of Azerbaijan (EIF-2010-1(1)-40/24-M-20).

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Authors and Affiliations

  1. Institute of Molecular Biology and Biotechnology, Azerbaijan National Academy of Sciences, 2a Matbuat Avenue, Baku, 1073, Azerbaijan

    Irada M. Huseynova, Samira M. Rustamova, Saftar Y. Suleymanov, Durna R. Aliyeva, Alamdar Ch. Mammadov & Jalal A. Aliyev

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  1. Irada M. Huseynova
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  2. Samira M. Rustamova
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  3. Saftar Y. Suleymanov
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  4. Durna R. Aliyeva
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  5. Alamdar Ch. Mammadov
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  6. Jalal A. Aliyev
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Correspondence to Irada M. Huseynova or Jalal A. Aliyev.

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Huseynova, I.M., Rustamova, S.M., Suleymanov, S.Y. et al. Drought-induced changes in photosynthetic apparatus and antioxidant components of wheat (Triticum durum Desf.) varieties. Photosynth Res 130, 215–223 (2016). https://doi.org/10.1007/s11120-016-0244-z

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  • DOI: https://doi.org/10.1007/s11120-016-0244-z

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