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Iron plaque formation and its effect on arsenic uptake by different genotypes of paddy rice

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Abstract

Background and aims

Iron plaque on roots has been hypothesized to be an effective restraint on the uptake of arsenic (As) by rice plants. Evaluating the formation of iron plaque and its effect on As uptake by various rice cultivars is valuable because selecting low As uptake rice cultivars results in reduced risks associated with rice consumption. This study examines iron plaque formation and its effect on As uptake by different genotypes of rice cultivars.

Methods

Hydroponic cultures were conducted in phytotron at day 25/night 20°C and the rice seedlings in fifth-leaf age were treated with Fe (II) at the levels of 0 and 100 mg L−1 in the Kimura B nutrient solutions for 14 days. The amount of iron plaque formation of 28 rice cultivars was determined by using the DCB extractable Fe of roots. Four cultivars representing high and low iron plaque formation capability, from indica and japonica respectively, were selected out of the 28 cultivars and processed for Fe and As treatments. After Fe treatments for 4 days, the seedlings were fed with As (III) at levels of 0, 0.5, and 1 mg L−1 for another 10 days. We were thus able to determine the amounts of iron plaque formation and the As content in iron plaque, roots, and shoots of the four tested cultivars.

Results

Iron plaque formation capability differed among tested twenty-eight rice cultivars. Feeding As to four tested cultivars enhanced iron plaque formation on roots; the As uptake by roots and shoots was decreased by the addition of Fe. Both the retention of As on iron plaque and the decrease of As uptake by the addition of Fe varied among tested cultivars and were not correlated with the iron plaque formation capability.

Conclusions

Iron plaque can sequestrate As on the roots and reduce rice’s As uptake. However, other factors also influence the As uptake, namely the differences in binding affinity of iron plaque to As, the existent As species in the rhizosphere, and the uptake capability of various As species by rice plants. These factors should also be considered when selecting low As uptake rice cultivars.

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Acknowledgments

The financial support from the National Science Council, Executive Yuan, Taiwan (grant no. NSC-98-2313-B-002-028-MY3) is sincerely appreciated.

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Authors and Affiliations

  1. Department of Agricultural Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., Taipei, 10617, Taiwan

    Chia-Hsing Lee, Yi-Cheng Hsieh, Tzu-Huei Lin & Dar-Yuan Lee

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  1. Chia-Hsing Lee
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  2. Yi-Cheng Hsieh
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  3. Tzu-Huei Lin
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  4. Dar-Yuan Lee
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Correspondence to Dar-Yuan Lee.

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Responsible Editor: Henk Schat.

Dr. Chia-Hsing Lee and Mr. Yi-Cheng Hsieh are equal contributors to this paper.

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Lee, CH., Hsieh, YC., Lin, TH. et al. Iron plaque formation and its effect on arsenic uptake by different genotypes of paddy rice. Plant Soil 363, 231–241 (2013). https://doi.org/10.1007/s11104-012-1308-2

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  • DOI: https://doi.org/10.1007/s11104-012-1308-2

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