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The small auxin-up RNA OsSAUR45 affects auxin synthesis and transport in rice

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This research is the first to demonstrate that OsSAUR45 is involved in plant growth though affecting auxin synthesis and transport by repressing OsYUCCA and OsPIN gene expression in rice.

Abstract

Small auxin-up RNAs (SAURs) comprise a large multigene family and are rapidly activated as part of the primary auxin response in plants. However, little is known about the role of SAURs in plant growth and development, especially in monocots. Here, we report the biological function of OsSAUR45 in the model plant rice (Oryza sativa). OsSAUR45 is expressed in a tissue-specific pattern and is localized to the cytoplasm. Rice lines overexpressing OsSAUR45 displayed pleiotropic developmental defects including reduced plant height and primary root length, fewer adventitious roots, narrower leaves, and reduced seed setting. Auxin levels and transport were reduced in the OsSAUR45 overexpression lines, potentially because of decreased expression of Flavin-binding monooxygenase family proteins (OsYUCCAs) and PIN-FORMED family proteins (OsPINs). Exogenous auxin application rapidly induced OsSAUR45 expression and partially restored the phenotype of rice lines overexpressing OsSAUR45. These results demonstrate that OsSAUR45 is involved in plant growth by affecting auxin synthesis and transport through the repression of OsYUCCA and OsPIN gene expression in rice.

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Acknowledgements

This work was financially supported by the National Natural Science Foundation (31371591, 31600562). We thank Professor Akio Miyao in the Rice Genome Resource Center, Japan for provision of the full-length cDNA clone of OsSAUR45.

Author contributions

Conceived and designed the experiment: Y-XX, D-AJ. Performed the experiments: Y-XX, M-ZX, YL, J-LF. Discussed the data: YH. Wrote the paper: Y-XX, D-AJ.

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Authors and Affiliations

  1. National Center for Tea Improvement, Tea Research Institute of the Chinese Academy of Agricultural Sciences/Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, 9 South Meiling Road, Hangzhou, 310008, China

    Yan-Xia Xu

  2. State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, 310058, China

    Yan-Xia Xu, Meng-Zhu Xiao, Yan Liu, Jun-Liang Fu, Yi He & De-An Jiang

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  1. Yan-Xia Xu
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  2. Meng-Zhu Xiao
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  3. Yan Liu
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Correspondence to Yan-Xia Xu or De-An Jiang.

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Xu, YX., Xiao, MZ., Liu, Y. et al. The small auxin-up RNA OsSAUR45 affects auxin synthesis and transport in rice. Plant Mol Biol 94, 97–107 (2017). https://doi.org/10.1007/s11103-017-0595-7

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