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Heme oxygenase 1 defects lead to reduced chlorophyll in Brassica napus

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Abstract

We previously described a Brassica napus chlorophyll-deficient mutant (ygl) with yellow-green seedling leaves and mapped the related gene, BnaC.YGL, to a 0.35 cM region. However, the molecular mechanisms involved in this chlorophyll defect are still unknown. In this study, the BnaC07.HO1 gene (equivalent to BnaC.YGL) was isolated by the candidate gene approach, and its function was confirmed by genetic complementation. Comparative sequencing analysis suggested that BnaC07.HO1 was lost in the mutant, while a long noncoding-RNA was inserted into the promoter of the homologous gene BnaA07.HO1. This insert was widely present in B. napus cultivars and down-regulated BnaA07.HO1 expression. BnaC07.HO1 was highly expressed in the seedling leaves and encoded heme oxygenase 1, which was localized in the chloroplast. Biochemical analysis showed that BnaC07.HO1 can catalyze heme conversion to form biliverdin IXα. RNA-seq analysis revealed that the loss of BnaC07.HO1 impaired tetrapyrrole metabolism, especially chlorophyll biosynthesis. According, the levels of chlorophyll intermediates were reduced in the ygl mutant. In addition, gene expression in multiple pathways was affected in ygl. These findings provide molecular evidences for the basis of the yellow-green leaf phenotype and further insights into the crucial role of HO1 in B. napus.

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Acknowledgements

We thank Yun Zhang, Yuanlong Wu, and Dongqin Li for technical support, and Qinghua Zhang and Yanjie Xie for helpful suggestions. No conflicts of interest are declared. This research was financially supported by grants from the Modern Agricultural Industrial Technology System (nycytx-00501), the National Science Foundation of China (31501340), the China Postdoctoral Science Foundation (2015M570647), the Natural Science Foundation of Hubei Province Key Program (2014CFA008) and the National Key Research and Development Program of China (2016YFD0101300).

Author Contributions

L.Z., X.Z., T.F., and J.W. designed the experiments. L.Z., Z.Y., J.G., and J.L. performed the experiments; J.T., J.S., C.M., and B.Y. contributed new reagents or analytic tools; L.Z. and J.W. analyzed the data and wrote the paper.

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  1. National Key Laboratory of Crop Genetic Improvement, National Sub-center of Rapeseed Improvement in Wuhan, Huazhong Agricultural University, Wuhan, 430070, China

    Lixia Zhu, Jie Gao, Jie Liu, Bin Yi, Chaozhi Ma, Jinxiong Shen, Jinxing Tu, Tingdong Fu & Jing Wen

  2. Shandong Key Laboratory of Greenhouse Vegetable Biology, Shandong Branch of National Vegetable Improvement Center, Institute of Vegetables and Flowers, Shandong Academy of Agricultural Sciences, Jinan, 250100, China

    Zonghui Yang

  3. Key Laboratory of Biology and Genetic Improvement of Oil Crops Oil Crops Research the Chinese Institute of Academy of Agricultural Sciences,, Ministry of Agriculture, Wuhan, 430062, China

    Xinhua Zeng

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  1. Lixia Zhu
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Correspondence to Jing Wen.

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Zhu, L., Yang, Z., Zeng, X. et al. Heme oxygenase 1 defects lead to reduced chlorophyll in Brassica napus . Plant Mol Biol 93, 579–592 (2017). https://doi.org/10.1007/s11103-017-0583-y

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