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Genome-wide analysis reveals phytohormone action during cassava storage root initiation

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Abstract

Development of storage roots is a process associated with a phase change from cell division and elongation to radial growth and accumulation of massive amounts of reserve substances such as starch. Knowledge of the regulation of cassava storage root formation has accumulated over time; however, gene regulation during the initiation and early stage of storage root development is still poorly understood. In this study, transcription profiling of fibrous, intermediate and storage roots at eight weeks old were investigated using a 60-mer-oligo microarray. Transcription and gene expression were found to be the key regulating processes during the transition stage from fibrous to intermediate roots, while homeostasis and signal transduction influenced regulation from intermediate roots to storage roots. Clustering analysis of significant genes and transcription factors (TF) indicated that a number of phytohormone-related TF were differentially expressed; therefore, phytohormone-related genes were assembled into a network of correlative nodes. We propose a model showing the relationship between KNOX1 and phytohormones during storage root initiation. Exogeneous treatment of phytohormones N 6-benzylaminopurine and 1-Naphthaleneacetic acid were used to induce the storage root initiation stage and to investigate expression patterns of the genes involved in storage root initiation. The results support the hypothesis that phytohormones are acting in concert to regulate the onset of cassava storage root development. Moreover, MeAGL20 is a factor that might play an important role at the onset of storage root initiation when the root tip becomes swollen.

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Acknowledgments

This work was partially supported by (1) Faculty of Science, Mahidol University, Thailand (2) The Thailand Research Fund (RSA5780030), Thailand and (3) RIKEN Center for Sustainable Resource Science under the Strategic Funds for the Promotion of Science and Technology of the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan. We thank Dr. Opas Boonseng (Rayong Field Crops Research Center, Ministry of Agriculture and Cooperatives, Rayong, Thailand) for KU50 field experiments, Assist. Prof. Paweena Traiperm (Department of Plant Science, Faculty of Science, Mahidol University, Bangkok, Thailand) for assistance in microscopic works, and Ms Somkid Bumee (Systems Biology and Bioinformatics Laboratory, Pilot Plant Development and Training Institute, King Mongkut’s University of Technology, Bangkok, Thailand) for computational support.

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Authors and Affiliations

  1. Department of Biotechnology, Center for Cassava Molecular Biotechnology, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand

    Punchapat Sojikul & Jarunya Narangajavana

  2. Bioinformatics and Systems Biology Program, School of Bioresources and Technology; Systems Biology and Bioinformatics Laboratory, Pilot Plant Development and Training Institute, King Mongkut’s University of Technology, Thonburi, 10150, Thailand

    Treenut Saithong & Saowalak Kalapanulak

  3. Department of Biopharmacy, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom, 73000, Thailand

    Nuttapat Pisuttinusart & Siripan Limsirichaikul

  4. Plant Genomic Network Research Team, RIKEN Center for Sustainable Resource Science, Kanagawa, Yokohama, 230-0045, Japan

    Maho Tanaka, Yoshinori Utsumi & Motoaki Seki

  5. Integrated Genome Informatics Research Unit, RIKEN Plant Science Center, Kanagawa, Yokohama, 230-0045, Japan

    Tetsuya Sakurai

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  1. Punchapat Sojikul
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Sojikul, P., Saithong, T., Kalapanulak, S. et al. Genome-wide analysis reveals phytohormone action during cassava storage root initiation. Plant Mol Biol 88, 531–543 (2015). https://doi.org/10.1007/s11103-015-0340-z

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