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The barley HvNAC6 transcription factor affects ABA accumulation and promotes basal resistance against powdery mildew

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Abstract

Barley HvNAC6 is a member of the plant-specific NAC (NAM, ATAF1,2, CUC2) transcription factor family and we have shown previously that it acts as a positive regulator of basal resistance in barley against the biotrophic pathogen Blumeria graminis f. sp. hordei (Bgh). In this study, we use a transgenic approach to constitutively silence HvNAC6 expression, using RNA interference (RNAi), to investigate the in vivo functions of HvNAC6 in basal resistance responses in barley in relation to the phytohormone ABA. The HvNAC6 RNAi plants displayed reduced HvNAC6 transcript levels and were more susceptible to Bgh than wild-type plants. Application of exogenous ABA increased basal resistance against Bgh in wild-type plants, but not in HvNAC6 RNAi plants, suggesting that ABA is a positive regulator of basal resistance which depends on HvNAC6. Silencing of HvNAC6 expression altered the light/dark rhythm of ABA levels which were, however, not influenced by Bgh inoculation. The expression of the two ABA biosynthetic genes HvNCED1 and HvNCED2 was compromised, and transcript levels of the ABA conjugating HvBG7 enzyme were elevated in the HvNAC6 RNAi lines, but this effect was not clearly associated with transgene-mediated resistance. Together, these data support a function of HvNAC6 as a regulator of ABA-mediated defence responses for maintenance of effective basal resistance against Bgh.

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Acknowledgments

Authors wish to acknowledge Marta de Torres-Zabala for technical advice on hormone extraction and Rebecca L. Neale for proofreading the manuscript. Research was financed by The Danish Council for Independent Research: Technology and Production Sciences (FTP) via the project, “Unravelling plant regulatory networks: NAC transcription factors in senescence and disease resistance,” and partial funding of Y.-J. C.’s PhD scholarship from the University of Copenhagen, Faculty of Science.

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Authors and Affiliations

  1. Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg C, Denmark

    Yan-Jun Chen, David B. Collinge & Michael F. Lyngkjær

  2. College of Life and Environmental Sciences, University of Exeter, Exeter, EX4 4QD, UK

    Venura Perera & Murray R. Grant

  3. Department of Molecular Biology and Genetics, Aarhus University, AU-Flakkebjerg, 4200, Slagelse, Denmark

    Michael W. Christiansen, Inger B. Holme & Per L. Gregersen

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  1. Yan-Jun Chen
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Chen, YJ., Perera, V., Christiansen, M.W. et al. The barley HvNAC6 transcription factor affects ABA accumulation and promotes basal resistance against powdery mildew. Plant Mol Biol 83, 577–590 (2013). https://doi.org/10.1007/s11103-013-0109-1

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  • DOI: https://doi.org/10.1007/s11103-013-0109-1

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