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Relationship between photosynthetic pigments and chlorophyll fluorescence in soybean under varying phosphorus nutrition at ambient and elevated CO2

  • Original Paper
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Photosynthetica

Abstract

To assess the relationship between chlorophyll (Chl) fluorescence (CF) and photosynthetic pigments, soybean was grown under varying phosphorus (P) nutrition at ambient and elevated CO2 (EC). The EC stimulated, but P deficiency decreased plant height, node numbers, and leaf area concomitantly with the rates of stem elongation, node addition, and leaf area expansion. Under P deficiency, CF parameters and pigments declined except that carotenoids (Car) were relatively stable indicating its role in photoprotection. The CF parameters were strongly related with Chl concentration but not with Chl a/b or Car. However, total Chl/Car showed the strongest association with CF parameters such as quantum efficiency and yield of photosystem II. This relationship was not affected by CO2 treatment. The high correlation between CF and total Chl/Car underscores the significance of the quantification of both, Chl and Car concentrations, to understand the photochemistry and underlying processes of photoprotection and mechanisms of excess energy dissipation in a given environment.

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Abbreviations

AC:

ambient CO2

Car:

carotenoids

Chl:

chlorophyll

EC:

elevated CO2

DAP:

days after planting

DAT:

days after treatment

Fv'/Fm':

quantum efficiency by oxidized (open) PSII reaction center in light or PSII efficiency

MLAER:

main-stem leaf area expansion rate

MNAR:

main-stem node addition rate

MSER:

main-stem elongation rate

MSLA:

main-stem leaf area

MSNN:

main-stem node number

PS:

photosystem

TChl:

total chlorophylls

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Acknowledgments

Authors would like to thank Mr. Darryl Baxam (Engineering Technician) for help in maintaining the growth chambers.

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Authors and Affiliations

  1. Crop Systems and Global Change Laboratory, USDA-ARS, Beltsville, MD, 20705, USA

    S. K. Singh, V. R. Reddy, D. H. Fleisher & D. J. Timlin

  2. Wye Research and Education Center, University of Maryland, Beltsville, MD, USA

    S. K. Singh

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  1. S. K. Singh
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  2. V. R. Reddy
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  3. D. H. Fleisher
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  4. D. J. Timlin
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Correspondence to S. K. Singh.

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Singh, S.K., Reddy, V.R., Fleisher, D.H. et al. Relationship between photosynthetic pigments and chlorophyll fluorescence in soybean under varying phosphorus nutrition at ambient and elevated CO2 . Photosynthetica 55, 421–433 (2017). https://doi.org/10.1007/s11099-016-0657-0

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  • DOI: https://doi.org/10.1007/s11099-016-0657-0

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