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Preparation of BMP-2 Containing Bovine Serum Albumin (BSA) Nanoparticles Stabilized by Polymer Coating

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Abstract

Purpose

The purpose of this study was to investigate the preparation process of bone morphogenetic protein-2 (BMP-2) containing bovine serum albumin (BSA) nanoparticles (NPs), and to assess the bioactivity of BMP-2 encapsulated in such NPs.

Methods

The NPs were prepared by a coacervation method, and the effects of process parameters on NP size and polydispersity were examined. Polymer coated NPs were characterized with respect to amount of adsorbed polymer, particle size and zeta potential. Using bone marrow stromal cells (BMSC), biocompatibility of the NPs was investigated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) Assay, and bioactivity of the encapsulated BMP-2 was investigated by alkaline phosphatase (ALP) induction and calcification.

Results

The size of NPs could be controlled in the 50–400 nm range by process parameters including BSA concentration, non-solvent:solvent ratio and pH value. After coating with cationic polymers, the particle size and zeta potential were significantly increased. MTT assay indicated no toxicity of both the uncoated and coated NPs on BMSC. Based on ALP induction and calcification, full retention of BMP-2 bioactivity was retained in the polymer-coated NPs.

Conclusions

This study described a preparation procedure for BSA NPs with controllable particle size, and such polymer-coated BSA NPs are promising delivery agents for local and systemic administration of BMP-2 in bone regeneration.

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Acknowledgements

The authors thank Xiaoyue Lin and Cezary Kucharski (Faculty of Engineering, University of Alberta) for technical help with cell culture studies. This project was financially supported by an operating grant from the Canadian Institutes of Health Research (CIHR). Guilin Wang is financially supported by a scholarship from the China Scholarship Council. Kevin Siggers is financially supported by a graduate studentship from the Natural Sciences and Engineering Research Council of Canada and a CIHR Team Grant (PI: Derrick Rancourt). BMP-2 was kindly provided by Dr. Walter Sehald (University of Würzburg, Germany)

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Authors and Affiliations

  1. Department of Chemical and Materials Engineering, Faculty of Engineering, University of Alberta, Edmonton, Alberta, T6G 2G6, Canada

    Guilin Wang, Kevin Siggers, Sufeng Zhang, Zhenghe Xu & Hasan Uludağ

  2. Department of Surgery, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, T6G 2G6, Canada

    Hongxing Jiang

  3. Departments of Orthopaedic Surgery and Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA

    Ronald F. Zernicke

  4. Department of Cell Biology and Anatomy, Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada

    John Matyas

  5. Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, T6G 2G6, Canada

    Hasan Uludağ

  6. Department of Biomedical Engineering, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, T6G 2G6, Canada

    Hasan Uludağ

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  1. Guilin Wang
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Correspondence to Hasan Uludağ.

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Wang, G., Siggers, K., Zhang, S. et al. Preparation of BMP-2 Containing Bovine Serum Albumin (BSA) Nanoparticles Stabilized by Polymer Coating. Pharm Res 25, 2896–2909 (2008). https://doi.org/10.1007/s11095-008-9692-2

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  • DOI: https://doi.org/10.1007/s11095-008-9692-2

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