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Expression of Src Suppressed C Kinase Substrate in Rat Neural Tissues During Inflammation

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Abstract

Src-suppressed C kinase substrate (SSeCKS), an in vivo and in vitro protein kinase C substrate, is a major lipopolysaccharide (LPS) response protein which markedly upregulated in several organs, including brain, lung, heart, kidney etc., indicating a possible role of SSeCKS in inflammatory process. However, the expression and biological function of SSeCKS during neuronal inflammation remains to be elucidated, so we established an inflammatory model injected with LPS to investigate the gene expression patterns of SSeCKS in neural tissues by using TaqMan quantitative real-time PCR and immunohistochemistry in rat. Real-time PCR showed that LPS stimulated the expression of SSeCKS mRNA in a dose- and time-dependent manner in sciatic nerves, spinal cords and dorsal root ganglions. Immunohistochemistry showed that SSeCKS colocalized with nerve fibers in sciatic nerve after LPS administration, but there was no colocalization between SSeCKS and Schwann cells. In addition, SSeCKS colocalized with neurons which existed in dorsal root ganglions and spinal cords. These findings indicated that SSeCKS might play some important roles in sciatic nerve fibers and neurons in spinal cords and dorsal root ganglions after LPS injection.

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Abbreviations

SSeCKS:

Src-suppressed C kinase substrate

LPS:

Lipopolysaccharide

DRG:

Dorsal root ganglion

β-1,4-GalT I:

β-1,4-Galactosyltransferase I

PCR:

Polymerase chain reaction

TNF-α:

Tumor necrosis factor-α

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Acknowledgments

This work was supported by the National Natural Scientific Foundation of China (31370803, 30772242 and 81171777), Natural Scientific Foundation of Jiangsu Province (BK2011188), Post-doctoral Program of Jiangsu Province (1201028C), Application Study Program of Nantong City (BK2013004), Early Pre-research Program of Nantong University (10ZY013) and Doctoral Program of Nantong University(12B035).

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Authors and Affiliations

  1. Research Center of Clinical Medicine, Affiliated Hospital of Nantong University, Nantong University, 19 Qi-xiu Road, Nantong, 226001, Jiangsu, China

    Meijuan Yan, Jianmei Zhao & Dengfu Yao

  2. The Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, 226001, China

    Meijuan Yan, Shunxin Zhu, Xiaoyi Shao & Li Zhang

  3. The Affiliated Jiangyin Hospital of Nantong University, Nantong, China

    Heng Gao

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  1. Meijuan Yan
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Correspondence to Heng Gao or Dengfu Yao.

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Yan, M., Zhao, J., Zhu, S. et al. Expression of Src Suppressed C Kinase Substrate in Rat Neural Tissues During Inflammation. Neurochem Res 39, 748–757 (2014). https://doi.org/10.1007/s11064-014-1265-7

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  • DOI: https://doi.org/10.1007/s11064-014-1265-7

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