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Genetic analysis of C5a receptors in neutrophils from patients with familial Mediterranean fever

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Abstract

Familial Mediterranean fever (FMF) is an autoinflammatory disease, characterized by MEFV gene mutations and self-limited recurrent episodes of fever and localized serositis. Complement system is a key regulator of the inflammatory process. The aim of this study was to investigate the genetic alterations and mRNA expression pattern of C5aR and C5L2 genes in neutrophils from attack-free FMF patients. No mutations were observed in the two receptors’ genes, while the genetic alteration observed in the C5aR1 gene was identified as N279 K polymorphic variant. Furthermore, lower mRNA expression of C5L2 gene was observed in neutrophils from FMF patients compared to control subjects. The binding capacity of rhC5a and the ability to produce reactive oxygen species was similar in neutrophils from healthy subjects and FMF patients and independent of the presence of N279 K polymorphism or mRNA expression of C5L2.

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Acknowledgments

The authors would like to thank Dr. John D. Lambris for his general support and for providing GFP-rhC5a, C5aR antagonist-PMX53 and the inactive scrambled peptide-control PMX53. This study was supported by the Governing Board of University Hospital of Alexandroupolis, the Hellenic Ministry of Education and General Secretariat for Research and Technology (ESPA, “synergasia” project, No. 898).

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Authors and Affiliations

  1. First Department of Internal Medicine, Democritus University of Thrace, Alexandroupolis, Greece

    Eirini Apostolidou, Konstantinos Kambas, Akrivi Chrysanthopoulou, Ioannis Kourtzelis, Konstantinos Ritis & Ioannis Mitroulis

  2. Department of Immunology and Histocompatibility, University of Thessaly, Larissa, Greece

    Matthaios Speletas

  3. University Hospital of Alexandroupolis, Dragana, Alexandroupolis, Greece

    Ioannis Mitroulis

  4. University General Hospital of Alexandroupolis, Alexandroupolis, Greece

    Eirini Apostolidou, Konstantinos Kambas, Akrivi Chrysanthopoulou, Ioannis Kourtzelis, Konstantinos Ritis & Ioannis Mitroulis

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  1. Eirini Apostolidou
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  2. Konstantinos Kambas
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  3. Akrivi Chrysanthopoulou
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  4. Ioannis Kourtzelis
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  5. Matthaios Speletas
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  6. Konstantinos Ritis
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  7. Ioannis Mitroulis
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Correspondence to Ioannis Mitroulis.

Additional information

Eirini Apostolidou and Konstantinos Kambas contributed equally to this work.

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Apostolidou, E., Kambas, K., Chrysanthopoulou, A. et al. Genetic analysis of C5a receptors in neutrophils from patients with familial Mediterranean fever. Mol Biol Rep 39, 5503–5510 (2012). https://doi.org/10.1007/s11033-011-1353-6

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  • DOI: https://doi.org/10.1007/s11033-011-1353-6

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