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Genetic variation at mtDNA and microsatellite loci in Chinese longsnout catfish (Leiocassis longirostris)

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Abstract

Genetic variability and population structure of the Chinese longsnout catfish Leiocassis longirostris Günther in the Yangtze River was examined with mitochondrial control region sequences and nuclear microsatellite markers. A 705-bp segment of the mitochondrial DNA control region was sequenced from 132 samples, which identified a total of 61 haplotypes. The Chinese longsnout catfish in the Yangtze River was characterized with high haplotype diversity (h = 0.9770 ± 0.0041) but low nucleotide diversity (π = 0.0081 ± 0.0043). Median-joining network analysis revealed a star-shaped pattern and mismatch distribution analysis found a smooth unimodal distribution, which suggested that this species in the Yangtze River underwent a population expansion following bottlenecks and/or they originated from a small size of founding population. It was estimated that the possible time of population expansion was 139,000–435,000 years before present, a time period in the middle Pleistocene. The analysis of molecular variance and phylogenetic reconstructions did not detect significant geographic structure between different river sections. This pattern of genetic variation was further evidenced with nuclear microsatellite markers. The genetic differentiation between above and below the Gezhouba Dam and Three Gorges Dam is very small at mitochondrial and nuclear levels, which suggested that these recently developed dams might have not significantly resulted in population genetic fragmentation in the Chinese longsnout catfish. However, the potential exacerbation of genetic structuring by the dams should not be overlooked in the future.

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Acknowledgments

This research was supported by the Program for New Century Excellent Talents in University (NCET-07-0445), the Ministry of Education of China, the Specialized Research Fund for the Doctoral Program of Higher Education (SRFDP 20060319002), the Ministry of Education of China, the major project of the Natural Science Foundation of the Jiangsu Higher Education Institutions of China (07KJA18016), and the key project of National Natural Science Foundation of China (30830018).

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  1. Mingsong Xiao

    Present address: Anhui Science and Technology University, Fengyang, 233100, China

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  1. Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, 210046, China

    Guang Yang, Mingsong Xiao, Yanyan Yu & Shixia Xu

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Supplementary Fig. 1

Alignment of mtDNA control region haplotypes of variable positions and distribution of mtDNA control region haplotypes in sampling localities (TIFF 88 kb)

Supplementary Fig. 1, continued (TIFF 67 kb)

Supplementary Fig. 1, continued (TIFF 73 kb)

Supplementary Fig. 1, continued (TIFF 65 kb)

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Yang, G., Xiao, M., Yu, Y. et al. Genetic variation at mtDNA and microsatellite loci in Chinese longsnout catfish (Leiocassis longirostris). Mol Biol Rep 39, 4605–4617 (2012). https://doi.org/10.1007/s11033-011-1252-x

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