Abstract
The physiological balance of juvenile hormone (JH) in insects depends on its biosynthesis and degradation pathway. Three key enzymes namely, juvenile hormone esterase (JHE), juvenile hormone epoxide hydrolase (JHEH) and juvenile hormone diol kinase (JHDK) are required for degradation in insects. Our present results showed that JHE and JHEH exhibited expression in almost all the tissues. This indicated that JHE and JHEH might degrade JH simultaneously. In addition, the highest levels of JHDK were observed in the midgut, with trace level being found in the malpighian tubule and haemocytes. Since the midgut is a digestive organ and not a JH target, it was hypothesized that both JHE and JHEH hydrolyzed JH to JH diol (JHd) which was then transported to midgut and hydrolyzed further by JHDK, to be finally excreted out of the body. Also the expression studies on JH degradation enzymes in different tissues and stages indicated that the activities of the three enzymes are specific and coincident with the JH functions in silkworm, Bombyx mori L.
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References
Riddiford LM (1994) Cellular and molecular actions of juvenile hormones. I: general considerations and premetamorphic actions. Adv in Insect Physiol 24:213–273
Riddiford LM (1996) Juvenile hormone: the status of its “status quo” action. Arch Insect Biochem Physiol 32(3–4):271–286
Hirai M, Kamimura M, Kikuchi K, Yasukochi Y, Kiuchi M, Shinoda T, Shiotsuki T (2002) cDNA cloning and characterization of Bombyx mori juvenile hormone esterase: an inducible gene by the imidazole insect growth regulator KK-42. Insect Biochem Mol Biol 32(6):627–635
Debernard S, Morisseau C, Severson TF, Feng L, Wojtasek H, Prestwich GD, Hammock BD (1998) Expression and characterization of the recombinant juvenile hormone epoxide hydrolase (JHEH) from Manduca sexta. Insect Biochem Mol Biol 28(5–6):409–419
Zhang QR, Xu WH, Chen FS, Li S (2005) Molecular and biochemical characterization of juvenile hormone epoxide hydrolase from the silkworm, Bombyx mori. Insect Biochem Mol Biol 35(2):153–164
Maxwell RA, Welch WH, Schooley DA (2002) Juvenile hormone diol kinase. I. Purification, characterization, and substrate specificity of juvenile hormone-selective diol kinase from Manduca sexta. J Biol Chem 277(24):21874–21881
Maxwell RA, Welch WH, Horodyski FM, Schegg KM, Schooley DA (2002) Juvenile hormone diol kinase. II. Sequencing, cloning, and molecular modeling of juvenile hormone-selective diol kinase from Manduca sexta. J Biol Chem 277(24):21882–21890
Li S, Zhang QR, WH Xu, Schooley DA (2005) Juvenile hormone diol kinase, a calcium-binding protein with kinase activity, from the silkworm, Bombyx mori. Insect Biochem Mol Biol 35(11):1235–1248
Share MR, Roe RM (1988) A partition assay for the simultaneous determination of insect juvenile hormone esterase and epoxide hydrolase activity. Anal Biochem 169(1):81–88
Feng QL, Ladd TR, Tomkins BL, Sundaram M, Sohi SS, Retnakaran A, Davey KG, Palli SR (1999) Spruce budworm (Choristoneura fumiferana) juvenile hormone esterase: hormonal regulation, developmental expression and cDNA cloning. Mol Cell Endocrinol 148(1–2):95–108
Hanzlik TN, Abdel-Aal YA, Harshman LG, Hammock BD (1989) Isolation and sequencing of cDNA clones coding for juvenile hormone esterase from Heliothis virescens. Evidence for a catalytic mechanism for the serine carboxylesterases different from that of the serine proteases. J Biol Chem 264(21):12419–12425
Hinton AC, Hammock BD (2001) Purification of juvenile hormone esterase and molecular cloning of the cDNA from Manduca sexta. Insect Biochem Mol Biol 32(1):57–66
Abdel-Aal YA, Hammock BD (1986) Transition state analogs as ligands for affinity purification of juvenile hormone esterase. Science 233(4768):1073–1076
Hajos JP, Vermunt AM, Zuidema D, Kulcsar P, Varjas L, de Kort CA, Zavodszky P, Vlak JM (1999) Dissecting insect development: baculovirus-mediated gene silencing in insects. Insect Mol Biol 8(4):539–544
Tan A, Tanaka H, Tamura T, Shiotsuki T (2005) Precocious metamorphosis in transgenic silkworms overexpressing juvenile hormone esterase. Proc Natl Acad Sci USA 102(33):11751–11756
Wojtasek H, Prestwich GD (1996) An insect juvenile hormone-specific epoxide hydrolase is related to vertebrate microsomal epoxide hydrolases. Biochem Biophys Res Commun 220(2):323–329
VanHook Harris S, Marin Thompson D, Linderman RJ, Tomalski MD, Roe RM (1999) Cloning and expression of a novel juvenile hormone-metabolizing epoxide hydrolase during larval-pupal metamorphosis of the cabbage looper, Trichoplusia ni. Insect Mol Biol 8(1):85–96
Keiser KC, Brandt KS, Silver GM, Wisnewski N (2002) Cloning, partial purification and in vivo developmental profile of expression of the juvenile hormone epoxide hydrolase of Ctenocephalides felis. Arch Insect Biochem Physiol 50(4):191–206
Severson TF, Goodrow MH, Morisseau C, Dowdy DL, Hammock BD (2002) Urea and amide-based inhibitors of the juvenile hormone epoxide hydrolase of the tobacco hornworm (Manduca sexta: Sphingidae). Insect Biochem Mol Biol 32(12):1741–1756
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The work was supported by the National Basic Research Program of China under grand no. 2005CB121003 and the Hi-Tech Research and Development Program of China (No. 2008AA10Z132 and No. 2006AA10A119) and the National Natural Science Foundation of China (No. 30972141/C120110).
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Hua-jun, Y., Fang, Z., Awquib, S. et al. Expression pattern of enzymes related to juvenile hormone metabolism in the silkworm, Bombyx mori L.. Mol Biol Rep 38, 4337–4342 (2011). https://doi.org/10.1007/s11033-010-0559-3
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DOI: https://doi.org/10.1007/s11033-010-0559-3