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Is a single nucleotide polymorphism a risk factor for lung cancer in the matrix metalloproteinase-2 promoter?

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Abstract

We aimed to investigate the association of polymorphism frequencies of MMP-2 C1306T and MMP-2 plasma enzyme activity in lung cancer patients. In this study 300 genomic DNA (200 lung cancer patients + 100 no lung cancer) were analyzed. Polymorphisms were determined by using polymerase chain reaction-restriction fragment length polymorphism (RFLP) and electrophoresis. Plasma MMP-2 enzyme activity levels were measured by using ELISA. Sex, asbestos expose and smoking might be risk factors for lung cancer. The frequencies of C1306T genotypes in controls CC 65%, CT 32%, TT 3% and in patients CC 61%, CT 37%, TT2 % were found. It was determined that CC genotype frequency increase significantly in patients and controls. Plasma MMP-2 enzyme activity levels were increased in lung cancer patients according to controls. Finally, we claimed that determining of MMP-2 enzyme level can be used as a marker in lung cancer.

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Acknowledgments

This study was supported by a grant of the Research Foundation of University of Eskişehir Osmangazi, Turkey (Grant No. 200611003)

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Authors and Affiliations

  1. Faculty of Medicine, Department of Medical Biology, Eskisehir Osmangazi University, 26480, Eskisehir, Turkey

    Bayramoglu Ayşegül, Gunes Hasan Veysi, Degirmenci Irfan, Akın Azra & Kurt Hulyam

  2. Faculty of Medicine, Department of Pulmonary Diseases, Eskisehir Osmangazi University, Eskisehir, Turkey

    Metintas Muzaffer

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  1. Bayramoglu Ayşegül
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  2. Gunes Hasan Veysi
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  3. Metintas Muzaffer
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  4. Degirmenci Irfan
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  5. Akın Azra
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Correspondence to Bayramoglu Ayşegül.

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Ayşegül, B., Veysi, G.H., Muzaffer, M. et al. Is a single nucleotide polymorphism a risk factor for lung cancer in the matrix metalloproteinase-2 promoter?. Mol Biol Rep 38, 1469–1474 (2011). https://doi.org/10.1007/s11033-010-0253-5

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  • DOI: https://doi.org/10.1007/s11033-010-0253-5

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