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Identification and fine mapping of pm5.1: a recessive gene for powdery mildew resistance in cucumber (Cucumis sativus L.)

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Abstract

Powdery mildew is one of the most important diseases of cucumber (Cucumis sativus L.). For many years, researchers have studied and attempted to isolate resistance genes for this disease for breeding powdery mildew-resistant cucumber. In this study, bulked segregant analysis and an initial quantitative trait loci (QTLs) analysis based on a local linkage map were performed using an F2 population. A major locus on the long arm of chromosome 5 was identified for powdery mildew resistance and named pm5.1. For fine mapping of the pm5.1 locus, BC3F1 and BC2F2 populations that segregated only at the target genomic region were developed by marker-assisted selection. The location of the pm5.1 locus was confirmed by QTL analysis using a subset of the BC3F1 population and was delimited in a 1.8-cM interval between flanking markers UW065011 and UW065190. Using the large BC3F1 (n = 480) and BC2F2 (n = 483) populations, pm5.1 was mapped to a ~170-kb region between markers UW065021 and UW065094. Based on the annotations for the genes in this region, a MLO-like gene was identified as the most likely candidate for the pm5.1. Sequence alignment analysis of the MLO-like genes from susceptible and resistant cucumber lines revealed two types of mutations in this candidate gene that may result in recessive resistance to powdery mildew in the resistant cucumber lines.

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Acknowledgments

We thank Lihuang Zhu (Institute of Genetics and Developmental Biology, Chinese Academy of Sciences) for technical assistance. We thank Dr. Sanwen Huang (Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences) and Dr. Yiqun Weng (University of Wisconsin) for providing the cucumber genome data. We also thank Dr. Dehua Ma (Tianjin Derit Seeds Company Ltd) for providing the cucumber material. This work was supported by the National High Technology Research and Development Program of China (863 Program) (No. 2012AA100104), the National Key Basic Research Projects (973 Program) (No. 2012CB113900), the National Natural Science Foundation of China (No. 31471892), and Shanghai Graduate Education and Innovation Program (Horticulture).

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Authors and Affiliations

  1. School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai, China

    Jingtao Nie, Huanle He, Jialin Peng, Xuqin Yang, Beibei Bie, Junlong Zhao, Yunli Wang, Jun-Song Pan & Run Cai

  2. Department of Horticulture, Shenyang Agriculture University, Shenyang, China

    Longting Si

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  1. Jingtao Nie
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  2. Huanle He
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  3. Jialin Peng
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  9. Jun-Song Pan
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Correspondence to Jun-Song Pan or Run Cai.

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Nie, J., He, H., Peng, J. et al. Identification and fine mapping of pm5.1: a recessive gene for powdery mildew resistance in cucumber (Cucumis sativus L.). Mol Breeding 35, 7 (2015). https://doi.org/10.1007/s11032-015-0206-8

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