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Isolation and S-genotyping application of S-allelic polymorphic MdSLFBs in apple (Malus domestica Borkh.)

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Abstract

Apple (Malus domestica Borkh.) possesses gametophytic self-incompatibility (GSI) which is controlled by S-RNase in the pistil as well as a pollen S-determinant that has not been well characterized. The identification of S-locus F-box brother (SFBB) genes, which are good candidates for the pollen S-determinant in apple and pear, indicated the presence of multiple S-allelic polymorphic F-box genes at the S-locus. In apple, two SFBB gene groups have been described, while there are at least three groups in pear. In this report, we identified five MdSLFB (S-RNase-linked F-box) genes from four different S-genotypes of apple. These genes showed pollen- and S-allele-specific expression with a high polymorphism among S-alleles. The phylogenetic tree suggested that some of them belong to SFBBα or β groups as described previously, while others appear to be different from SFBBs. In particular, the presence of MdSLFB3 and MdSLFB9 suggested that there are more S-allelic polymorphic F-box gene groups in the S-locus besides α and β. Based on the sequence polymorphism of MdSLFBs, we developed an S-genotyping system for apple cultivars. In addition, we isolated twelve MdSLFB-like genes, which showed pollen-specific expression without S-allelic polymorphism.

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Acknowledgments

We are grateful to two anonymous reviewers for their careful reading and critical comments, as well as to Dr. YongBiao Xue (Institute of Genetics and Developmental Biology, Chinese Academy of Sciences) for critical reading. This work was supported by a grant from the National Natural Science Foundation of China (No. 30671443).

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Authors and Affiliations

  1. Laboratory of Fruit Tree Cell and Molecular Breeding, China Agricultural University, 100193, Beijing, China

    Maofu Li, Kaikai Zhu, Songling Bai & Tianzhong Li

  2. Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, China

    Maofu Li

  3. The United Graduate School of Agricultural Science, Iwate University, Morioka, 020-8550, Japan

    Songling Bai

  4. Fruit Tree Institute of Liaoning Province, 110161, Xiongyue, Liaoning, China

    Zhi Liu

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  1. Maofu Li
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  2. Kaikai Zhu
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  3. Songling Bai
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  4. Zhi Liu
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  5. Tianzhong Li
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Correspondence to Tianzhong Li.

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Maofu Li and Kaikai Zhu contributed equally to this work.

Electronic supplementary material

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11032_2010_9471_MOESM1_ESM.doc

Sequence data from this article have been deposited with the GenBank Data Libraries under the following accession nos.: Dc1: GU345806 (MdSLFB24), Dc2: GU345807 (MdSLFBL11), Dc3: GU345808 (MdSLFBL12), Dc4: GU345809 (MdSLFBL13), Dc5: GU345810 (MdSLFBL14), Gs1: GU345811 (MdSLFB3), Gs2: GU345812 (MdSLFBL3), Gs3: GU345813 (MdSLFBL4), Id1: GU345814 (MdSLFB7-2),Id2: GU345815 (MdSLFBL10), Jt4: GU345816 (MdSLFB7-1), Mp1: GU345817 (MdSLFBL8), Mp2: GU345818 (MdSLFBL9), Rc1: GU345819 (MdSLFBL6), Rc2: GU345820 (MdSLFBL7), Ss1: GU345821 (MdSLFB5), Ss2: GU345822 (MdSLFBL5). (DOC 160 kb)

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Li, M., Zhu, K., Bai, S. et al. Isolation and S-genotyping application of S-allelic polymorphic MdSLFBs in apple (Malus domestica Borkh.). Mol Breeding 28, 171–180 (2011). https://doi.org/10.1007/s11032-010-9471-8

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