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Multiple lines of genetic inquiry reveal effects of local and landscape factors on an amphibian metapopulation

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Abstract

Context

A central tenet of landscape ecology is that both characteristics of patches and the matrix between them influence functional connectivity. Landscape genetics seeks to evaluate functional connectivity by determining the role of spatial processes in the distribution of genetic diversity on the landscape. However, landscape genetics studies often consider only the landscape matrix, ignoring patch-level characteristics, and possibly missing significant drivers of functional connectivity.

Objectives

(1) Evaluate drivers of functional connectivity for an amphibian metapopulation, and (2) determine whether local characteristics are as important as landscape features to functional connectivity of this species.

Methods

We used gravity models to evaluate the evidence for hypothesized drivers of functional connectivity for Dryophytes wrightorum that included both local and landscape attributes and a novel combination of methods of genetic inquiry: landscape genetics and environmental DNA (eDNA). Hypothesized drivers of connectivity included effects of hydrology, canopy cover, and species interactions.

Results

Evidence weights indicated that stream networks were the most likely driver of functional connectivity, and connectivity along stream networks was positively correlated with gene flow. We also found a strong correlation between abundance of D. wrightorum from eDNA data and effective population size estimates from microsatellite data.

Conclusions

We found evidence that functional connectivity of D. wrightorum was strongly driven by stream networks, despite considering multiple local and landscape processes. This suggests that management of this species focused on landscape hydrologic connectivity as gene flow corridors while maintaining current local management action is likely to have a positive effect on species conservation.

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Acknowledgements

We would like to thank all participating members of the Distributed Graduate Course in Landscape Genetics 2018 for their constructive comments throughout this project and for supporting travel for all authors to come together and work on the project. We would also like thank Dr. William Peterman (Ohio State University) for detailed comments on earlier drafts and study design. Funding for ML Torres provided by University of Wyoming Underrepresented Domestic Minority fellowship, MA Murphy provided by National Institute of Food and Agriculture (NIFA SAES, University of Wyoming, project WYO-5360-14), and CS Goldberg provided by USDA National Institute of Food and Agriculture (Hatch Project WNP00827).

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Authors and Affiliations

  1. School of the Environment, Washington State University, Pullman, WA, 99164, USA

    Meghan B. Parsley & Caren S. Goldberg

  2. Department of Ecosystem Science and Management/Program in Ecology, University of Wyoming, Laramie, WY, 82071, USA

    Melanie L. Torres & Melanie A. Murphy

  3. Department of Environmental Conservation, University of Massachusetts, Amherst, MA, 01003, USA

    Shreya M. Banerjee

  4. Department of Biology, Woods Hole Oceanographic Institution, 266 Woods Hole Road, Woods Hole, MA, 02543, USA

    Zachary J. C. Tobias

  5. Department of Earth, Atmospheric and Planetary Sciences, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA, 02139, USA

    Zachary J. C. Tobias

  6. Department of Biological Sciences, Virginia Tech, 2125 West Campus Drive, Blacksburg, VA, 24061, USA

    Meryl C. Mims

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Parsley, M.B., Torres, M.L., Banerjee, S.M. et al. Multiple lines of genetic inquiry reveal effects of local and landscape factors on an amphibian metapopulation. Landscape Ecol 35, 319–335 (2020). https://doi.org/10.1007/s10980-019-00948-y

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