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Osseointegration around dental implants biofunctionalized with TGFβ-1 inhibitor peptides: an in vivo study in beagle dogs

  • Biocompatibility Studies
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Abstract

The aim of this study was to evaluate the effect of biofunctionalization with two TGF-β1 inhibitor peptides, P17 and P144, on osseointegration of CP-Ti dental implants. A total of 36 implants (VEGA, Klockner®) with 3.5 × 8 mm internal connection were used in this study, divided in three groups: (1) control group (n = 12), (2) implants which surfaces were biofunctionalized with P17 peptide inhibitor (n = 12), (3) implants with surfaces biofunctionalized by P144 peptide (n = 12). Three implants, one from each group, were inserted in both hemimandibles of 6 beagle dogs, 2 months after tooth extraction. Two animals were sacrificed at 2, 4 and 8 weeks post implant insertion, respectively. The samples were analyzed by Backscattering Scanning Electron Microscopy (BS-SEM) and histological analysis. Histomorphometric analysis of bone to implant contact (BIC), peri-implant bone fraction (BF) and interthread bone (IB) were carried out. Bone formation around implants measured by quantitative analysis, BS-SEM, was significantly higher in the P17-biofunctionalized implants, 4 and 8 weeks after the implantation. Histomorphometric analysis of BIC, BF and IB showed higher values in the P17-biofunctionalized group at initial stages of healing (2 weeks) and early osseointegration both at 4 and 8 weeks. For P144 biofunctionalized implants, the histomorphometric values obtained are also higher than control group. Accordingly, better results in the experimental groups were proven both by the quantitative and the qualitative analysis. Surface biofunctionalization with TGF-β1 inhibitor peptides, P17 and P144, resulted in better quantitative and qualitative parameters relative to implant osseointegration.

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Acknowledgements

The authors kindly acknowledge the collaboration of many individuals and institutions in the present manuscript. The present study was supported by the Research Cathedra Klockner-University of Granada. The experimental animals surgery and care was carried out with the valuable collaboration of Dr Albert Barba and Katrin Rappe of the surgical team in the Veterinary Faculty, Autonomous University of Barcelona; we would like to thank Ricardo Fernández and Allinson Olaechea for their participation during surgeries; the samples preparation and visualization was carried out by Ms Mónica Ortiz, from the Grup de Biomaterials, Biomecànica i Enginyeria de Teixits, Dept. de Ciència dels Materials i Enginyeria Metal·lúrgica and the Barcelona Research Center in MultiScale Science and Engineering, Universitat Politècnica de Catalunya (UPC). Ms Eva Sanchez, from the Human Anatomy and Embryology lab in the Bellvitge campus of the University of Barcelona is kindly acknowledged by her technical support to the management of the undecalcified samples. The authors are grateful to the Spanish Government and European Union FEDER by the concession of the project RTI2018–098075-B-C22.

Funding

The present study was supported totally by the Research Cathedra Klockner-University of Granada.

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Authors and Affiliations

  1. Oral Surgery and Implant Dentistry Department, School of Dentistry, University of Granada, Campus Universitario La Cartuja s/n, Barcelona, Spain

    Andrea Cirera

  2. Escola Universitària Salesiana de Sarrià – EUSS Autonomous University of Barcelona, Barcelona, Spain

    Pablo Sevilla

  3. Human Anatomy and Embryology Unit, DPyTEx, School of Medicine, University of Barcelona, Barcelona, Spain

    M. Cristina Manzanares

  4. Surgery Department, Veterinary School, Autonomous University of Barcelona, Bellaterra, Barcelona, Spain

    Jordi Franch

  5. Oral Surgery and Implant Dentistry Department, School of Dentistry, University of Granada, Barcelona, Spain

    Pablo Galindo-Moreno

  6. Universitat Internacional de Catalunya, Facultat de Medicina i Ciències de la Salut, Barcelona, Spain

    Javier Gil

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  1. Andrea Cirera
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Correspondence to Pablo Sevilla.

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Cirera, A., Sevilla, P., Manzanares, M.C. et al. Osseointegration around dental implants biofunctionalized with TGFβ-1 inhibitor peptides: an in vivo study in beagle dogs. J Mater Sci: Mater Med 31, 62 (2020). https://doi.org/10.1007/s10856-020-06397-3

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