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Comparative study of PCL-HAp and PCL-bioglass composite scaffolds for bone tissue engineering

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Abstract

The aim of this work is to compare the effect of hydroxyapatite (HAp) or bioglass (BG) nanoparticles in a polycaprolactone composite scaffold aimed to bone regeneration. To allow a comparison of the influence of both types of fillers, scaffolds made of PCL or composites containing up to 20 % by weight HAp or BG were obtained. Scaffolds showed acceptable mechanical properties for its use and high interconnected porosity apt for cellular colonization. To study the effect of the different materials on pre-osteoblast cells differentiation, samples with 5 % mineral reinforcement, were cultured for up to 28 days in osteogenic medium. Cells proliferated in all scaffolds. Nevertheless, differentiation levels for the selected markers were higher in pure PCL scaffolds than in the composites; inclusion of bioactive particles showed no positive effects on cell differentiation. In osteogenic culture conditions, the presence of bioactive particles is thus not necessary in order to observe good differentiation.

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Acknowledgments

JLGR acknowledges the support of the Spanish Ministry of Science and Education through project No. MAT2010-21611-C03-01 (including the FEDER financial support), and from Generalitat Valenciana, ACOMP/2012/075 Project.. Lebourg acknowledges the support of UPV through Project PAID-O6-10 and thanks CIBER-BBN for funding her post-doc research. CIBER-BBN is an initiative funded by the VI National R&D&i Plan 2008–2011, Iniciativa Ingenio 2010, Consolider Program, CIBER Actions and financed by the Instituto de Salud Carlos III with assistance from the European Regional Development Fund. J. Ródenas acknowledges the funding of his PhD by the Generalitat Valenciana through VALi+d Grant. The authors also wish to thank the microscopy service of Universidad Politécnica de Valencia as well as the confocal microscopy service of the Research Centre Principe Felipe for useful help and advice.

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Authors and Affiliations

  1. Center for Biomaterials and Tissue Engineering, Universidad Politécnica de Valencia, Edificio 8E, esc F, nivel 1. Camino de Vera s/n, 46022, Valencia, Spain

    Joaquín Ródenas-Rochina, José Luis Gómez Ribelles & Myriam Lebourg

  2. CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Zaragoza, Spain

    José Luis Gómez Ribelles & Myriam Lebourg

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  1. Joaquín Ródenas-Rochina
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  2. José Luis Gómez Ribelles
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Correspondence to Joaquín Ródenas-Rochina.

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Ródenas-Rochina, J., Ribelles, J.L.G. & Lebourg, M. Comparative study of PCL-HAp and PCL-bioglass composite scaffolds for bone tissue engineering. J Mater Sci: Mater Med 24, 1293–1308 (2013). https://doi.org/10.1007/s10856-013-4878-5

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