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Hydroxyapatite/gelatin/gellan sponges as nanocomposite scaffolds for bone reconstruction

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Abstract

The aim of this work was the morphological, physicochemical, mechanical and biological characterization of a new composite system, based on gelatin, gellan and hydroxyapatite, and mimicking the composition of natural bone. Porous scaffolds were prepared by freeze–drying technique, under three different conditions of freezing. The morphological analysis showed a homogeneous porosity, with well interconnected pores, for the sample which underwent a more rapid freezing. The elastic modulus of the same sample was close to that of the natural bone. The presence of interactions among the components was demonstrated through the physicochemical investigation. In addition, the infrared chemical imaging analysis pointed out the similarity among the composite scaffold and the natural bone, in terms of chemical composition, homogeneity, molecular interactions and structural conformation. Preliminary biological characterization showed a good adhesion and proliferation of human mesenchymal stem cells.

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Authors and Affiliations

  1. Department of Chemical Engineering, Industrial Chemistry and Materials Science, University of Pisa, Largo Lucio Lazzarino, 56122, Pisa, Italy

    Niccoletta Barbani, Riccardo Avvisati, Alessandro Sala & Elisabetta Rosellini

  2. Institute for Composite and Biomedical Materials, C.N.R., U.O.S. of Pisa, Largo Lucio Lazzarino, 56122, Pisa, Italy

    Giulio D. Guerra & Caterina Cristallini

  3. Cell Biology and Tissue Regeneration Laboratory, Immunohematology 2 Unit, Azienda Ospedaliera Universitaria Pisana, Via Paradisa 1, 56124, Pisa, Italy

    Patrizia Urciuoli

Authors
  1. Niccoletta Barbani
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  2. Giulio D. Guerra
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  3. Caterina Cristallini
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  4. Patrizia Urciuoli
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  5. Riccardo Avvisati
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  6. Alessandro Sala
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  7. Elisabetta Rosellini
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Correspondence to Niccoletta Barbani or Elisabetta Rosellini.

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Barbani, N., Guerra, G.D., Cristallini, C. et al. Hydroxyapatite/gelatin/gellan sponges as nanocomposite scaffolds for bone reconstruction. J Mater Sci: Mater Med 23, 51–61 (2012). https://doi.org/10.1007/s10856-011-4505-2

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  • DOI: https://doi.org/10.1007/s10856-011-4505-2

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