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Surface immobilized zoledronate improves screw fixation in rat bone: A new method for the coating of metal implants

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Abstract

Previous studies show that surface immobilized bisphosphonates improve the fixation of stainless steel screws in rat tibia after 2–8 weeks of implantation. We report here about the immobilization of a potent bisphosphonate, zoledronate, to crosslinked fibrinogen by the use of another technique, i.e. ethyl-dimethyl-aminopropylcarbodiimide (EDC)/imidazole immobilization. Bone fixation of zoledronate-coated screws was compared to screws coated with crosslinked fibrinogen only and ditto with EDC/N-hydroxy-succinimide immobilized pamidronate. Fixation in rat tibia was evaluated by a pull-out test at either 2 or 6 weeks after implantation. Both bisphosphonate coatings increased the pull-out force at both time points, and zoledronate showed a significantly higher pull-out force than pamidronate. To further evaluate the new coating technique we also performed a morphometric study, focusing on the area surrounding the implant. The zoledronate coating resulted in an increased bone density around the screws compared to controls. No pronounced increase was seen around the pamidronate coated screws. Together, the results demonstrate the possibility of obtaining a significant local therapeutic effect with minute amounts of surface immobilized zoledronate.

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Acknowledgments

This study was supported by the local strategic research project Materials in Medicine, funded by County Council of Östergötland and Linköpings Universitet, Sweden, The Swedish Research Council 2006-5647, Swedish Research Council (VR) and Trygg-Hansa Research foundation.

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Authors and Affiliations

  1. Experimental Orthopaedics, Department of Clinical and Experimental Medicine, Faculty of Medicine, Linköping University, 58183, Linköping, Sweden

    Therese Andersson, Fredrik Agholme & Per Aspenberg

  2. Department of Biomaterials, Institute for Clinical Sciences, Sahlgrenska Academy, Gothenburg University, 40530, Gothenburg, Sweden

    Pentti Tengvall

Authors
  1. Therese Andersson
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  2. Fredrik Agholme
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  3. Per Aspenberg
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  4. Pentti Tengvall
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Correspondence to Therese Andersson.

Additional information

Per Aspenberg and Pentti Tengvall have shares in a company with research related to the subject.

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Andersson, T., Agholme, F., Aspenberg, P. et al. Surface immobilized zoledronate improves screw fixation in rat bone: A new method for the coating of metal implants. J Mater Sci: Mater Med 21, 3029–3037 (2010). https://doi.org/10.1007/s10856-010-4154-x

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  • DOI: https://doi.org/10.1007/s10856-010-4154-x

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