Skip to main content

Advertisement

Log in

Interpreting Past Human Diets Using Stable Isotope Mixing Models—Best Practices for Data Acquisition

  • Published:
Journal of Archaeological Method and Theory Aims and scope Submit manuscript

Abstract

Using stable isotope mixing models (SIMMs) to quantify past diets is becoming increasingly common in archaeology. This study highlights important field-specific difficulties encountered by archaeologists in reconstructing palaeodiets using SIMMs. Focusing on the data acquisition stage, we discuss several issues that could confound dietary quantification if not accounted for. These issues are categorized under several broad categories: diagenesis, intra-individual variability, representativeness of both the consumers and sources, and other commonly encountered field-specific problems. We summarize these issues with a flow chart to help archaeologists to select the most appropriate samples for dietary reconstruction using SIMMs, thereby decreasing the probability that the outputs of the SIMM are inaccurate. We conclude by discussing the ways in which SIMMs may not be appropriate for all archaeological contexts, highlighting those areas that are likely to be the most problematic for end users.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

Data Availability

All simulated data are provided in the Supplementary files.

Code Availability

All codes used in this study are provided in the Supplementary files.

References

  • Albarella, U. (1999). ‘The Mystery of Husbandry’: Medieval Animals and the Problem of Integrating Historical and Archaeological Evidence. Antiquity, 73(282), 867–875.

    Article  Google Scholar 

  • Ambrose, S. (1990). Preparation and Characterization of Bone and Tooth Collagen for Isotopic Analysis. Journal of Archaeological Science, 17(4), 431–451.

    Article  Google Scholar 

  • Ambrose, S., & Norr, L. (1993). Experimental Evidence for the Relationship of the Carbon Isotope Ratios of Whole Diet and Dietary Protein to Those of Bone Collagen and Carbonate. In J. Lambert & G. Grupe (Eds.), Prehistoric Human Bone: Archaeology at the Molecular Level (pp. 1–37). Heidelberg: Springer.

    Google Scholar 

  • Ambrose, S., Buikstra, J., & Krueger, H. (2003). Status and Gender Differences in Diet at Mound 72, Cahokia, Revealed by Isotopic Analysis of Bone. Journal of Anthropological Archaeology, 22(3), 217–226.

    Article  Google Scholar 

  • Amundson, R., Austin, A. T., Schuur, E. A. G., Yoo, K., Matzek, V., Kendall, C., Uebersax, A., Brenner, D., & Baisden, W. T. (2003). Global patterns of the isotopic composition of soil and plant nitrogen. Global Biogeochemical Cycles, 17, 1031.

    Article  Google Scholar 

  • Austin, A. T., & Vitousek, P. M. (1998). Nutrient dynamics on a precipitation gradient in Hawai'i. Oecologia, 113(4), 519–529.

    Article  Google Scholar 

  • Badeck, F.-W., Tcherkez, G., Nogués, S., Piel, C., & Ghashghaie, J. (2005). Post-photosynthetic fractionation of stable carbon isotopes between plant organs—a widespread phenomenon. Rapid Communications in Mass Spectrometry, 19(11), 1381–1391.

    Article  Google Scholar 

  • Baumann, C., Bocherens, H., Drucker, D., & Conard, N. (2020). Fox Dietary Ecology as a Tracer of Human Impact on Pleistocene Ecosystems. PLoS ONE, 15(7), e0235692.

    Article  Google Scholar 

  • Bivand, R., Pebesma, E., & Gomez-Rubio, V. (2013). Applied Spatial Data Analysis with R. New York: Springer.

    Book  Google Scholar 

  • Black, H. D., Andrus, C. F. T., Lambert, W. J., Rick, T. C., & Gillikin, D. P. (2017). δ15N Values in Crassostrea virginica Shells Provides Early Direct Evidence for Nitrogen Loading to Chesapeake Bay. Scientific Reports, 7(1), 44241.

    Article  Google Scholar 

  • Bocherens, H., Drucker, D., & Taubald, H. (2011). Preservation of Bone Collagen Sulphur Isotopic Compositions in an Early Holocene River-bank Archaeological Site. Palaeogeography, Palaeoclimatology, Palaeoecology, 310(1-2), 32–38.

    Article  Google Scholar 

  • Bocherens, H., Drucker, D., Germonpre, M., Lázničková-Galetová, M., Naito, Y., Wißing, C., Brůžek, J., & Oliva, M. (2015). Reconstruction of the Gravettian food-web at Předmostí I using multi-isotopic tracking (13C, 15N, 34S) of bone collagen. Quaternary Research, 84, 96–105.

    Google Scholar 

  • Bogaard, A., Heaton, T., Poulton, P., & Merbach, I. (2007). The Impact of Manuring on Nitrogen Isotope Ratios in Cereals: Archaeological Implications for Reconstruction of Diet and Crop Management Practices. Journal of Archaeological Science, 34(3), 335–343.

    Article  Google Scholar 

  • Bownes, J., Ascough, P., Cook, G., Murray, I., & Bonsall, C. (2017). Using Stable Isotopes and a Bayesian Mixing Model (FRUITS) to Investigate Diet at the Early Neolithic Site of Carding Mill Bay, Scotland. Radiocarbon, 59(5), 1275–1294.

    Article  Google Scholar 

  • Brown, T. A., Nelson, D. E., Vogel, J. S., & Southon, J. R. (1988). Improved collagen extraction by modified Longin method. Radiocarbon, 30(2), 171–177.

    Article  Google Scholar 

  • Budd, P., Montgomery, J., Barreiro, B., & Thomas, R. (2000). Differential Diagenesis of Strontium in Archaeological Human Dental Tissues. Applied Geochemistry, 5, 687–694.

    Article  Google Scholar 

  • Bump, J. K., Fox-Dobbs, K., Bada, J. L., Koch, P. L., Peterson, R. O., & Vucetich, J. A. (2007). Stable isotopes, ecological integration and environmental change: wolves record atmospheric carbon isotope trend better than tree rings. Proceedings of the Royal Society B: Biological Sciences, 274, 2471–2480.

    Article  Google Scholar 

  • Chen, X., Hu, S., Hu, Y., Wang, W., Ma, Y., Lü, P., & Wang, C. (2016). Raising Practices of Neolithic Livestock Evidenced by Stable Isotope Analysis in the Wei River Valley, North China. International Journal of Osteoarchaeology, 26(1), 42–52.

    Article  Google Scholar 

  • Cheung, C., & Szpak, P. (2020). Interpreting Past Human Diets Using Stable Isotope Mixing Models. Journal of Archaeological Method and Theory. https://doi.org/10.1007/s10816-020-09492-5.

  • Cheung, C., Jing, Z., Tang, J., Weston, D., & Richards, M. (2017). Diets, Social Roles, and Geographical Origins of Sacrificial Victims at the Royal Cemetery at Yinxu, Shang China: New Evidence from Stable Carbon, Nitrogen, and Sulfur Isotope Analysis. Journal of Anthropological Archaeology, 48, 28–45.

    Article  Google Scholar 

  • Clark, C. T., Horstmann, L., & Misarti, N. (2017). Quantifying variability in stable carbon and nitrogen isotope ratios within the skeletons of marine mammals of the suborder Caniformia. Journal of Archaeological Science: Reports, 15, 393–400.

    Google Scholar 

  • Coltrain, J. B., Janetski, J. C., & Carlyle, S. W. (2007). The Stable- and Radio-Isotope Chemistry of Western Basketmaker Burials: Implications for Early Puebloan Diets and Origins. American Antiquity, 72(2), 301–321.

    Article  Google Scholar 

  • Cooper, C., Lupo, K., Matson, R., Lipe, W., Smith, C., & Richards, M. (2016). Short-Term Variability of Human Diet at Basketmaker II Turkey Pen Ruins, Utah: Insights from Bulk and Single Amino Acid Isotope Analysis of Hair. Journal of archaeological Science: Reports, 5, 10–18.

    Google Scholar 

  • Cordain, L., Miller, J., Eaton, S., Mann, N., Holt, S., & Speth, J. (2000). Plant-Animal Subsistence Ratios and Macronutrient Energy Estimations in Worldwide Hunter-Gatherer Diets. The American Journal of Clinical Nutrition, 71(3), 682–692.

    Article  Google Scholar 

  • Cox, G., & Sealy, J. (1997). Investigating Identity and Life Histories: Isotopic Analysis and Historical Documentation of Slave Skeletons Found on the Cape Town Foreshore, South Africa. International Journal of Historical Archaeology, 1(3), 207–224.

    Article  Google Scholar 

  • Curto, A., Mahoney, P., Maurer, A.-F., Barrocas-Dias, C., Fernandes, T., & Fahy, G. (2020). Effect of Different Healing Stages on Stable Isotope Ratios in Skeletal Lesions. American Journal of Physical Anthropology, 171(2), 285–297.

    Article  Google Scholar 

  • DeNiro, M. J. (1985). Postmortem preservation and alteration of in vivo bone collagen isotope ratios in relation to palaeodietary reconstruction. Nature, 317(6040), 806–809.

    Article  Google Scholar 

  • DeNiro, M. J., & Epstein, S. (1978). Influence of diet on the distribution of carbon isotopes in animals. Geochimica et Cosmochimica Acta, 42(5), 495–506.

    Article  Google Scholar 

  • Dobberstein, R., Collins, M., Craig, O., Taylor, G., Penkman, K., & Ritz-Timme, S. (2009). Archaeological Collagen: Why Worry about Collagen Diagenesis? Archaeological and Anthropological Sciences, 1(1), 31–42.

    Article  Google Scholar 

  • Ehleringer, J. R., Hall, A. E., & Farquhar, G. D. (Eds.). (1993). Stable Isotopes and Plant Carbon-Water Relations. San Diego: Academic Press.

    Google Scholar 

  • Farnsworth, P., Brady, J., DeNiro, M., & MacNeish, R. (1985). A Re-Evaluation of the Isotopic and Archaeological Reconstructions of Diet in the Tehuacan Valley. American Antiquity, 50(1), 102–116.

    Article  Google Scholar 

  • Farquhar, G. D., Hubick, K. T., Condon, A. G., & Richards, R. A. (1989). Carbon isotope fractionation and plant water-use efficiency. In P. W. Rundel, J. R. Ehleringer, & K. A. Nagy (Eds.), Stable Isotopes in Ecological Research (pp. 21–40). New York: Springer-Verlag.

    Chapter  Google Scholar 

  • Faust, A., & Weiss, E. (2005). Judah, Philistia, and the Mediterranean World: Reconstructing the Economic System of the Seventh Century B.C.E. Bulletin of the American Schools of Oriental Research, 338, 71–92.

    Article  Google Scholar 

  • Fernandes, R., Grootes, P., Nadeau, M.-J., & Nehlich, O. (2015). Quantitative diet reconstruction of a Neolithic population using a Bayesian mixing model (FRUITS): The case study of Ostorf (Germany). American Journal of Physical Anthropology, 158(2), 325–340.

    Article  Google Scholar 

  • Finucane, B., Agurto, P. M., & Isbell, W. H. (2006). Human and animal diet at Conchopata, Peru: stable isotope evidence for maize agriculture and animal management practices during the Middle Horizon. Journal of Archaeological Science, 33(12), 1766–1776.

    Article  Google Scholar 

  • Fiorentino, G., Ferrio, J. P., Bogaard, A., Araus, J. L., & Riehl, S. (2015). Stable isotopes in archaeobotanical research. Vegetation History and Archaeobotany, 24(1), 215–227.

    Article  Google Scholar 

  • Fraser, I., Meier-Augenstein, W., & Kalin, R. (2006). The Role of Stable Isotopes in Human Identification: A Longitudinal Study into the Variability of Isotopic Signals in Human Hair and Nails. Rapid Communications in Mass Spectrometry, 20(7), 1109–1116.

    Article  Google Scholar 

  • Galloway, J. N., & Cowling, E. B. (2002). Reactive Nitrogen and The World: 200 Years of Change. AMBIO: A Journal of the Human Environment, 31(2), 64–71.

    Article  Google Scholar 

  • Garvie-Lok, S., Varney, T., & Katzenberg, M. (2004). Preparation of Bone Carbonate for Stable Isotope Analysis: the Effects of Treatment Time and Acid Concentration. Journal of Archaeological Science, 31(6), 763–776.

    Article  Google Scholar 

  • Gineyts, E., Cloos, P., Borel, O., Grimaud, L., Delmas, P., & Garnero, P. (2000). Racemization and Isomerization of Type I Collagen C-telopeptides in Human Bone and Soft Tissues: Assessment of Tissue Turnover. Biochemical Journal, 345(3), 481–485.

    Article  Google Scholar 

  • Guiry, E. (2019). Complexities of Stable Carbon and Nitrogen Isotope Biogeochemistry in Ancient Freshwater Ecosystems: Implications for the Study of Past Subsistence and Environmental Change. Frontiers in Ecology and Evolution, 7.

  • Guiry, E., & Szpak, P. (2020). Quality Control for Modern Bone Collagen Stable Carbon and Nitrogen Isotope Measurements. Methods in Ecology and Evolution, 11(9), 1049–1060.

    Article  Google Scholar 

  • Guiry, E., Noël, S., Tourigny, E., & Grimes, V. (2012). A Stable Isotope Method for Identifying Transatlantic Origin of Pig (Sus scrofa) Remains at French and English Fishing Stations in Newfoundland. Journal of Archaeological Science, 39(7), 2012–2022.

    Article  Google Scholar 

  • Guiry, E. J., Szpak, P., & Richards, M. P. (2016). Effects of lipid extraction and ultrafiltration on stable carbon and nitrogen isotopic compositions of fish bone collagen. Rapid Communications in Mass Spectrometry, 30(13), 1591–1600.

    Article  Google Scholar 

  • Hanawalt, B. (1986). The Ties that Bound Peasant Families in Medieval England. New York: Oxford University Press.

    Google Scholar 

  • Harbeck, M., & Grupe, G. (2009). Experimental Chemical Degradation Compared to Natural Diagenetic Alteration of Collage: Implications for Collagen Quality Indicators for Stable Isotope Analysis. Archaeological and Anthropological Sciences, 1(1), 43–57.

    Article  Google Scholar 

  • Harrower, M. (2010). Geographic Information Systems (GIS) Hydrological Modeling in Archaeology: An Example from the Origins of Irrigation in Southwest Arabia (Yemen). Journal of Archaeological Science, 37(7), 1447–1452.

    Article  Google Scholar 

  • Hedges, R., Clement, J., Thomas, D., & O'Connell, T. (2007). Collagen Turnover in the Adult Femoral Mid-Shaft: Modeled from Anthropogenic Radiocarbon Tracer Measurements. American Journal of Physical Anthropology, 133(2), 808–816.

    Article  Google Scholar 

  • Hijamns, R. (2019). raster: Geographic Data Analysis and Modeling. R package version 2.9-23. https://CRAN.R-project.org/package=raster.

  • Højte, J. (2006). The Archaeological Evidence for Fish Processing in the Black Sea Region. In T. Bekker-Nielsen (Ed.), Ancient Fishing and Fish Processing in the Black Sea Region (pp. 133–160). Aarhus: Aarhus University Press.

    Google Scholar 

  • Hu, Y., Ambrose, S., & Wang, C. (2006). Stable Isotopic Analysis of Human Bones from Jiahu Site, Henan, China: Implications for the Transition to Agriculture. Journal of Archaeological Science, 33(9), 1319–1330.

    Article  Google Scholar 

  • Itahashi, Y., Chikaraishi, Y., Ohkouchi, N., & Yoneda, M. (2014). Refinement of Reconstructed Ancient Food Webs Based on the Nitrogen Isotopic Compositions of Amino Acids from Bone Collagen: A Case Study of Archaeological Herbivores from Tell Ain el-Kerkh, Syria. Geochemical Journal, 48(4), e15–e19.

    Article  Google Scholar 

  • Jaouen, K., Richards, M., Le Cabec, A., Welker, F., Rendu, W., Hublin, J.-J., Soressi, M., & Talamo, S. (2019). Exceptionally High δ15N Values in Collagen Single Amino Acids Confirm Neandertals as High-Trophic Level Carnivores. Proceedings of the National Academy of Sciences, 116(11), 4928–4933.

    Article  Google Scholar 

  • Jim, S., Ambrose, S., & Evershed, R. (2004). Stable Carbon Isotopic Evidence for Differences in the Dietary Origin of Bone Cholesterol, Collagen and Apatite: Implications for Their Use in Palaeodietary Reconstruction. Geochimica et Cosmochimica Acta, 68(1), 61–72.

    Article  Google Scholar 

  • Jones, G. (1998). Distinguishing Food from Fodder in the Archaeobotanical Record. Environmental Archaeology, 1(1), 95–98.

    Article  Google Scholar 

  • Jørkov, M., Heinemeier, J., & Lynnerup, N. (2007). Evaluating Bone Collagen Extraction Methods for Stable Isotope Analysis in Dietary Studies. Journal of Archaeological Science, 34(11), 1824–1829.

    Article  Google Scholar 

  • Katzenberg, M., & Lovell, N. (1999). Stable Isotope Variation in Pathological Bone. International Journal of Osteoarchaeology, 9(5), 316–324.

    Article  Google Scholar 

  • Katzenberg, M. A., Schwarcz, H. P., Knyf, M., & Melbye, F. J. (1995). Stable Isotope Evidence for Maize Horticulture and Paleodiet in Southern Ontario, Canada. American Antiquity, 60(2), 335–350.

    Article  Google Scholar 

  • Kempster, B., Zanette, L., Longstaffe, F., MacDougall-Shackleton, S., Wingfield, J., & Clinchy, M. (2007). Do Stable Isotopes Reflect Nutritional Stress? Results from a Laboratory Experiment on Song Sparrows. Oecologia, 151(3), 365–371.

    Article  Google Scholar 

  • van Klinken, G. (1999). Bone Collagen Quality Indicators for Palaeodietary and Radiocarbon Measurements. Journal of Archaeological Science, 26(6), 687–695.

    Article  Google Scholar 

  • van Klinken, G. J., & Hedges, R. E. M. (1995). Experiments on Collagen-Humic Interactions: Speed of Humic Uptake, and Effects of Diverse Chemical Treatments. Journal of Archaeological Science, 22(2), 263–270.

    Article  Google Scholar 

  • Kohl, D. H., & Shearer, G. (1980). Isotopic Fractionation Associated With Symbiotic N2 Fixation and Uptake of NO3 by Plants. Plant Physiology, 66(1), 51–56.

    Article  Google Scholar 

  • Kohn, M. J., & Cerling, T. E. (2002). Stable Isotope Compositions of Biological Apatite. In M. J. Kohn, J. F. Rakovan, & J. M. Hughes (Eds.), Phosphates: Geochemical, Geobiological, and Materials Importance (pp. 455–488). Washington, D.C.: Mineralogical Society of America.

    Chapter  Google Scholar 

  • Kontopoulos, I., Presslee, S., Penkman, K., & Collins, M. (2018). Preparation of Bone Powder for FTIR-ATR Analysis: The Particle Size Effect. Vibrational Spectroscopy, 99, 167–177.

    Article  Google Scholar 

  • Kurle, C. M., & Worthy, G. A. J. (2002). Stable nitrogen and carbon isotope ratios in multiple tissues of the northern fur seal Callorhinus ursinus: implications for dietary and migratory reconstructions. Marine Ecology Progress Series, 236, 289–300.

    Article  Google Scholar 

  • Ladurie, M. (1976). The Peasants of Languedoc. Urbana and Chicago: University of Illinois Press.

    Google Scholar 

  • Lamb, A., Evans, J., Buckley, R., & Appleby, J. (2014). Multi-Isotope Analysis Demonstrates Significant Lifestyle Changes in King Richard III. Journal of Archaeological Science, 50, 559–565.

    Article  Google Scholar 

  • Lambert, P., Gagnon, C., Billman, B., Katzenberg, M., Carcelén, J., & Tykot, R. (2012). Bone Chemistry at Cerro Oreja: A Stable Isotope Perspective on the Development of a Regional Economy in the Moche Valley, Peru during the Early Intermediate Period. Latin American Antiquity, 23(2), 144–166.

    Article  Google Scholar 

  • Lehn, C., Mützel, E., & Rossmann, A. (2011). Multi-Element Stable Isotope Analysis of H, C, N and S in Hair and Nails of Contemporary Human Remains. International Journal of Legal Medicine, 125(5), 695–706.

    Article  Google Scholar 

  • Lightfoot, E., Ustunkaya, M., Przelomska, N., O'Connell, T., Hunt, H., Jones, M., & Petrie, C. (2020). Carbon and Nitrogen Isotopic Variability in Foxtail Millet (Setaria italica) with Watering Regime. Rapid Communications in Mass Spectrometry, 34, e8615.

    Article  Google Scholar 

  • Lowe, B. (1997). The Trade and Production of Garum and Its Role in the Provincial Economy of Hispania Tarraconensis. Edinburgh: University of Edinburgh.

    Google Scholar 

  • Lyman, R. L. (1994). Vertebrate Taphonomy. Cambridge: Cambridge University Press.

    Book  Google Scholar 

  • Mann, N. (2007). Meat in the Human Diet: An Anthropological Perspective. Journal of the Dietitians Association of Australia, 64, S102–S107.

    Google Scholar 

  • Martínez del Rio, C., Sabat, P., Anderson-Sprecher, R., & Gonzalez, S. P. (2009). Dietary and isotopic specialization: the isotopic niche of three Cinclodes ovenbirds. Oecologia, 161(1), 149–159.

    Article  Google Scholar 

  • McMahon, K. W., Hamady, L. L., & Thorrold, S. R. (2013). Ocean ecogeochemistry: A review. Oceanography and Marine Biology, An Annual Review, 51, 327–374.

    Google Scholar 

  • Meier-Augenstein, W., & Kemp, H. (2012). Stable Isotope Analysis: Hair and Nails. In A. Jamieson & A. Moenssens (Eds.), Wiley Encyclopedia of Forensic Science. https://doi.org/10.1002/9780470061589.fsa1043.

  • Miksicek, C. H. (1987). Formation Processes of the Archaeobotanical Record. In M. B. Schiffer (Ed.), Advances in Archaeological Method and Theory (pp. 211–247). San Diego: Academic Press.

    Chapter  Google Scholar 

  • Minnis, P. E. (1981). Seeds in Archaeological Sites: Sources and Some Interpretive Problems. American Antiquity, 46(1), 143–152.

    Article  Google Scholar 

  • Misarti, N., Gier, E., Finney, B., Barnes, K., & McCarthy, M. (2017). Compound-specific amino acid δ15N values in archaeological shell: Assessing diagenetic integrity and potential for isotopic baseline reconstruction. Rapid Communications in Mass Spectrometry, 31(22), 1881–1891.

    Article  Google Scholar 

  • Moreno, A. (2007). Feeding the Democracy: The Athenian Grain Supply in the Fifth and Fourth Centuries BC. Oxford: Oxford University Press.

    Book  Google Scholar 

  • Naito, Y., Chikaraishi, Y., Ohkouchi, N., Drucker, D., & Bocherens, H. (2013). Nitrogen Isotopic Composition of Collagen Amino Acids as an Indicator of Aquatic Resource Consumption: Insights from Mesolithic and Epipalaeolithic Archaeological Sites in France. World Archaeology, 45(3), 338–359.

    Article  Google Scholar 

  • Nestle, M. (1999). Animal v. Plant Foods in Human Diets and Health: Is The Historical Record Unequivocal? Proceedings of the Nutrition Society, 58, 211–218.

    Article  Google Scholar 

  • Netherly, P. J. (1977). Local Level Lords on the North Coast of Peru. Unpublished Ph.D. Dissertation. Ithaca: Cornell University.

  • Newsome, S., Phillips, D., Culleton, B., Guilderson, T., & Koch, P. (2004). Dietary Reconstruction of an Early to Middle Holocene Human Population from the Central California Coast: Insights from Advanced Stable Isotope Mixing Models. Journal of Archaeological Science, 31(8), 1101–1115.

    Article  Google Scholar 

  • Nicholson, R. A. (1996). Fish bone diagenesis in different soils. Archaeofauna, 5, 79–91.

    Google Scholar 

  • O'Connell, T., Hedges, R., Healey, M., & Simpson, A. (2001). Isotopic Comparison of Hair, Nail and Bone: Modern Analyses. Journal of Archaeological Science, 28(11), 1247–1255.

    Article  Google Scholar 

  • Oczkowski, A. J., Nixon, S. W., Granger, S. L., El-Sayed, A.-F. M., & McKinney, R. A. (2009). Anthropogenic enhancement of Egypt's Mediterranean fishery. Proceedings of the National Academy of Sciences, 106, 1364–1367.

    Article  Google Scholar 

  • Pearson, J., & Grove, M. (2013). Counting sheep: sample size and statistical inference in stable isotope analysis and palaeodietary reconstruction. World Archaeology, 45(3), 373–387.

    Article  Google Scholar 

  • Pearson, J. A., Buitenhuis, H., Hedges, R. E. M., Martin, L., Russell, N., & Twiss, K. C. (2007). New light on early caprine herding strategies from isotope analysis: a case study from Neolithic Anatolia. Journal of Archaeological Science, 34(12), 2170–2179.

    Article  Google Scholar 

  • Pebesma, E., & Bivand, R. (2005). Classes and Methods for Spatial Data in R. R News, 5(2), 1–21.

  • Pellegrini, M., & Snoeck, C. (2016). Comparing Bioapatite Carbonate Pre-Treatments for Isotopic Measurements: Part 2 — Impact on Carbon and Oxygen Isotope Compositions. Chemical Geology, 420, 88–96.

    Article  Google Scholar 

  • Phillips, R. (1993). Starchy Legumes in Human Nutrition, Health, and Culture. Plant Foods for Human Nutrition, 44(3), 195–211.

    Article  Google Scholar 

  • Phillips, D., Newsome, S., & Gregg, J. (2005). Combining Sources in Stable Isotope Mixing Models: Alternative Methods. Oecologia, 144(4), 520–527.

    Article  Google Scholar 

  • Post, D., Layman, C., Arrington, D., Takimoto, G., Quattrochi, J., & Montaña, C. (2007). Getting to the fat of the matter: models, methods and assumptions for dealing with lipids in stable isotope analyses. Oecologia, 152(1), 179–189.

    Article  Google Scholar 

  • Reinman, F. (1967). Fishing: An Aspect of Oceanic Economy: An Archaeological Approach. Fieldiana. Anthropology, 56, 99–208.

    Google Scholar 

  • Reitsema, L., Crews, D., & Polcyn, M. (2010). Preliminary Evidence for Medieval Polish Diet from Carbon and Nitrogen Stable Isotopes. Journal of Archaeological Science, 37(7), 1413–1423.

    Article  Google Scholar 

  • Renfrew, J. (2003). Archaeology and the Origins of Wine Production. In M. Sandler & R. Pinder (Eds.), Wine: A Scientific Exploration (pp. 56–69). London: Taylor & Francis.

    Google Scholar 

  • Richards, M. P., & Hedges, R. E. M. (1999). Stable Isotope Evidence for Similarities in the Types of Marine Foods Used by Late Mesolithic Humans at Sites Along the Atlantic Coast of Europe. Journal of Archaeological Science, 26(6), 717–722.

    Article  Google Scholar 

  • Ruiz-Cooley, R. I., & Gerrodette, T. (2012). Tracking large-scale latitudinal patterns of δ13C and δ15N along the E Pacific using epi-mesopelagic squid as indicators. Ecosphere, 3, 1–17.

    Article  Google Scholar 

  • Saragoça, P., Maurer, A.-F., Šoberl, L., Lopes, M. d. C., Alfenim, R., Leandro, I., Umbelino, C., Fernandes, T., Valente, M., Ribeiro, S., Santos, J., Janeiro, A., & Barrocas, C. (2016). Stable Isotope and Multi-Analytical Investigation of Monte da Cegonha: A Late Antiquity Population in Southern Portugal. Journal of archaeological Science: Reports, 9, 728–742.

    Google Scholar 

  • Scott, M. (2020). Chew the Fat: An Examination of the Preservation of Fatty Acids in Archaeological Bone. M.A. Thesis. Trent University.

  • Skippington, J., Veth, P., Manne, T., & Slack, M. (2019). Preanalytical Processing of Archaeological Mammal Enamel Apatite Carbonates for Stable Isotope Investigations: A Comparative Analysis of the Effect of Acid Treatment on Samples from Northwest Australia. International Journal of Osteoarchaeology, 29(5), 760–771.

    Article  Google Scholar 

  • Smith, B. (1991). Standards of Human Tooth Formation and Dental Age Assessment. In M. A. Kelley & C. S. Larsen (Eds.), Advances in Dental Anthropology (pp. 143–168). New York: Wiley-Liss.

  • Somerville, A., Goldstein, P., Baitzel, S., Bruwelheide, K., Dahlstedt, A., Yzurdiaga, L., Raubenheimer, S., Knudson, K., & Schoeninger, M. (2015). Diet and Gender in the Tiwanaku Colonies: Stable Isotope Analysis of Human Bone Collagen and Apatite from Moquegua, Peru. American Journal of Physical Anthropology, 158(3), 408–422.

    Article  Google Scholar 

  • Stafford, T. W. J., Brendel, K., & Duhamel, R. C. (1988). Radiocarbon, 13C and 15N analysis of fossil bone: Removal of humates with XAD-2 resin. Geochimica et Cosmochimica Acta, 52(9), 2257–2267.

    Article  Google Scholar 

  • Stevens, R., Lister, A., & Hedges, R. (2006). Predicting diet, trophic level and palaeoecology from bone stable isotope analysis: a comparative study of five red deer populations. Oecologia, 149(1), 12–21.

    Article  Google Scholar 

  • Stewart, K. M., & Wigen, R. J. (2003). Screen size and the need for reinterpretation: a case study from the Northwest Coast. Bulletin of the Florida Museum of Natural History, 44, 27–34.

    Google Scholar 

  • Stock, B., & Semmens, B. (2016). Unifying Error Structures in Commonly Used Biotracer Mixing Models. Ecology, 97(10), 2562–2569.

    Article  Google Scholar 

  • Stock, B., Jackson, A., Ward, E., Parnell, A., Phillips, D., & Semmens, B. (2018). Analyzing Mixing Systems using A New Generation of Bayesian Tracer Mixing Models. PeerJ, 6, e5096.

    Article  Google Scholar 

  • Styring, A. K., Charles, M., Fantone, F., Hald, M. M., McMahon, A., Meadow, R. H., Nicholls, G. K., Patel, A. K., Pitre, M. C., Smith, A., Sołtysiak, A., Stein, G., Weber, J. A., Weiss, H., & Bogaard, A. (2017). Isotope evidence for agricultural extensification reveals how the world's first cities were fed. Nature Plants, 3(6), 17076.

    Article  Google Scholar 

  • Styring, A. K., Knipper, C., Müller-Scheeßel, N., Grupe, G., & Bogaard, A. (2018). The Proof is in the Pudding: Crop Isotope Analysis Provides Direct Insights into Agricultural Production and Consumption. Environmental Archaeology, 1–12. https://doi.org/10.1080/14614103.2018.1497832.

  • Syväranta, J., Martino, A., Kopp, D., Céréghino, R., & Santoul, F. (2010). Freezing and chemical preservatives alter the stable isotope values of carbon and nitrogen of the Asiatic clam (Corbicula fluminea). Hydrobiologia, 658, 383–388.

    Article  Google Scholar 

  • Syväranta, J., Lensu, A., Marjomäki, T. J., Oksanen, S., & Jones, R. I. (2013). An Empirical Evaluation of the Utility of Convex Hull and Standard Ellipse Areas for Assessing Population Niche Widths from Stable Isotope Data. PLOS One, 8(2), e56094.

    Article  Google Scholar 

  • Szpak, P. (2011). Fish bone chemistry and ultrastructure: implications for taphonomy and stable isotope analysis. Journal of Archaeological Science, 38(12), 3358–3372.

    Article  Google Scholar 

  • Szpak, P. (2014). Complexities of Nitrogen Isotope Biogeochemistry in Plant-Soil Systems: Implications for the Study of Ancient Agricultural and Animal Management Practices. Frontiers in Plant Science, 5, 288.

    Article  Google Scholar 

  • Szpak, P., Longstaffe, F. J., Millaire, J.-F., & White, C. D. (2012). Stable Isotope Biogeochemistry of Seabird Guano Fertilization: Results from Growth Chamber Studies with Maize (Zea mays). PLOS One, 7(3), e33741.

    Article  Google Scholar 

  • Szpak, P., Millaire, J.-F., White, C. D., & Longstaffe, F. J. (2014). Small scale camelid husbandry on the north coast of Peru (Virú Valley): Insight from stable isotope analysis. Journal of Anthropological Archaeology, 36, 110–129.

    Article  Google Scholar 

  • Szpak, P., Krippner, K., & Richards, M. (2017a). Effects of Sodium Hydroxide Treatment and Ultrafiltration on the Removal of Humic Contaminants from Archaeological Bone. International Journal of Osteoarchaeology, 27(6), 1070–1077.

    Article  Google Scholar 

  • Szpak, P., Metcalfe, J. Z., & Macdonald, R. A. (2017b). Best Practices for Calibrating and Reporting Stable Isotope Measurements in Archaeology. Journal of Archaeological Science: Reports, 13, 609–616.

    Google Scholar 

  • Szpak, P., Millaire, J.-F., Chapdelaine, C., White, C. D., & Longstaffe, F. J. (2019). An Integrated isotopic study of Early Intermediate Period camelid husbandry in the Santa Valley, Perú. Environmental Archaeology, 25, 279–295.

    Article  Google Scholar 

  • Tafuri, M. A., Craig, O. E., & Canci, A. (2009). Stable isotope evidence for the consumption of millet and other plants in Bronze Age Italy. American Journal of Physical Anthropology, 139(2), 146–153.

    Article  Google Scholar 

  • Trakadas, A. (2006). The Archaeological Evidence for Fish Processing in the Western Mediterranean. In T. Bekker-Nielsen (Ed.), Ancient Fishing and Fish Processing in the Black Sea Region (pp. 47–82). Aarhus: Aarhus University Press.

    Google Scholar 

  • Tykot, R., van der Merwe, N., & Hammond, N. (1996). Stable Isotope Analysis of Bone Collagen, Bone Apatite, and Tooth Enamel in the Reconstruction of Human Diet. In M. V. Orna (Ed.), Archaeological Chemistry. ACS Symposium series (pp. 355–365). https://doi.org/10.1021/bk-1996-0625.ch025.

  • USDA (2019). FoodData Central, USDA Agricultural Research Service. https://fdc.nal.usda.gov/.

  • Vaiglova, P., Halstead, P., Pappa, M., Triantaphyllou, S., Valamoti, S., Evans, J., Fraser, R., Karkanas, P., Kay, A., Lee-Thorp, J., & Bogaard, A. (2018). Of cattle and feasts: Multi-isotope investigation of animal husbandry and communal feasting at Neolithic Makriyalos, northern Greece. PLoS ONE, 13(6), e0194474.

    Article  Google Scholar 

  • Van Neer, W., Lernau, O., Friedman, R., Mumford, G., Poblóme, J., & Waelkens, M. (2004). Fish Remains from Archaeological Sites as Indicators of Former Trade Connections in the Eastern Mediterranean. Paléorient, 30(1), 101–147.

    Article  Google Scholar 

  • Verburg, P. (2007). The need to correct for the Suess effect in the application of δ13C in sediment of autotrophic Lake Tanganyika, as a productivity proxy in the Anthropocene. Journal of Paleolimnology, 37(4), 591–602.

    Article  Google Scholar 

  • Warinner, C., & Tuross, N. (2009). Alkaline Cooking and Stable Isotope Tissue-Diet Spacing in Swine: Archaeological Implications. Journal of Archaeological Science, 36(8), 1690–1697.

    Article  Google Scholar 

  • Webb, E., White, C., & Longstaffe, F. (2014). Investigating Inherent Differences in Isotopic Composition between Human Bone and Enamel Bioapatite: Implications for Reconstructing Residential Histories. Journal of Archaeological Science, 50, 97–107.

    Article  Google Scholar 

  • Webb, E., Honch, N., Dunn, P., Eriksson, G., Lidén, K., & Evershed, R. (2015). Compound-Specific Amino Acid Isotopic Proxies for Detecting Freshwater Resource Consumption. Journal of Archaeological Science, 63, 104–114.

    Article  Google Scholar 

  • White, C. D., Healy, P. F., & Schwarcz, H. P. (1993). Intensive Agriculture, Social Status, and Maya Diet at Pacbitun, Belize. Journal of Anthropological Research, 49(4), 347–375.

    Article  Google Scholar 

  • Wißing, C., Rougier, H., Crevecoeur, I., Germonpré, M., Naito, Y., Semal, P., & Bocherens, H. (2016). Isotopic evidence for dietary ecology of late Neandertals in North-Western Europe. Quaternary International, 411, 327–345.

    Article  Google Scholar 

  • Zazzo, A., Balasse, M., & Patterson, W. (2006). The Reconstruction of Mammal Individual History: Refining High-Resolution Isotope Record in Bovine Tooth Dentine. Journal of Archaeological Science, 33(8), 1177–1187.

    Article  Google Scholar 

  • Zhuang, Y., Lee, H., & Kidder, T. (2016). The Cradle of Heaven-Human Induction Idealism: Agricultural Intensification, Environmental Consequences and Social Responses in Han China and Three-Kingdoms Korea. World Archaeology, 48(4), 563–585.

    Article  Google Scholar 

Download references

Acknowledgement

The authors wish to thank the anonymous reviewers for their helpful comments.

Funding

CC is supported by the Agence Nationale de la Recherche (Project-ANR-17-CE27-0023 “NEOGENRE”). PS is supported by the Canada Research Chairs program.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Christina Cheung.

Ethics declarations

Conflict of Interest

The authors declare that they have no conflict of interest.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

ESM 1

(XLSX 10 kb)

ESM 2

(R 7 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Cheung, C., Szpak, P. Interpreting Past Human Diets Using Stable Isotope Mixing Models—Best Practices for Data Acquisition. J Archaeol Method Theory 29, 138–161 (2022). https://doi.org/10.1007/s10816-021-09514-w

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10816-021-09514-w

Keywords

Navigation