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Comparison of the regenerative effects of bone marrow/adipose-derived stem cells in the Asherman model following local or systemic administration

  • Reproductive Physiology and Disease
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

Cell therapy is a promising strategy for the treatment of Asherman’s syndrome (AS), but the origin of these cells and injection route influence the therapeutic effect and complications of cell therapy. Herein, we compared the effects of systemic or local intrauterine injection of bone marrow or adipose-derived mesenchymal stem cells (BMSCs/AMSCs) on the endometrium in a rat model of AS.

Methods

After induction of AS in adult Wistar rats, the CM-Dil-positive BMSCs or AMSCs were injected either locally or intravenously. After 3 weeks, endometrial thickness, collagen deposition, cell migration, and VEGF expression were evaluated using histochemistry/immunofluorescence studies.

Results

In all stem cell-treated groups, an ameliorative effect on the damaged endometrium was noted. Collagen deposition diminished in both groups (IV and local injection) compared to the AS model. In rats injected locally with MSC, fibrosis decreased compared to the other groups. Moreover, endometrial thickness increased in the groups that received local injection of BMSCs and AMSCs more than the IV-transplanted AMSCs group. Immunofluorescent staining demonstrated that although the systemic transplantation of BMSCs was more effective than the other groups on VEGF expression, it led to the lowest number of CM-Dil+ stem cells in the damaged endometrium.

Conclusion

Stem cell transplantation may reconstruct the damaged endometrium, but it is recommended to select the most effective stem cells and injection route. Because the removal of the fibrosis and the replacement of the epithelia cells is an effective therapeutic strategy for AS, in this study, we conclude that the local injection of AMSCs is more appropriate than BMSCs to treat AS.

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Acknowledgments

This study was supported by the Hamadan University of Medical Sciences (9804042566).

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Authors and Affiliations

  1. Department of Anatomy, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

    Farhad Monsef, Tayebe Artimani, Zohreh Alizadeh, Mahdi Ramazani & Sara Soleimani Asl

  2. Endometrium and Endometriosis Research Centre, Hamadan University of Medical Sciences, Hamadan, Iran

    Tayebe Artimani, Zohreh Alizadeh, Mahdi Ramazani, Mahnaz Yavangi & Sara Soleimani Asl

  3. Department of Immunology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

    Ghasem Solgi

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  1. Farhad Monsef
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Correspondence to Sara Soleimani Asl.

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Monsef, F., Artimani, T., Alizadeh, Z. et al. Comparison of the regenerative effects of bone marrow/adipose-derived stem cells in the Asherman model following local or systemic administration. J Assist Reprod Genet 37, 1861–1868 (2020). https://doi.org/10.1007/s10815-020-01856-w

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  • DOI: https://doi.org/10.1007/s10815-020-01856-w

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