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Association of GRP78 promoter polymorphisms and serum GRP78 level with risk of asthenozoospermia

  • Genetics
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

The aim of this study was undertaken to investigate the association of 78-kDa glucose-regulated protein (GRP78) gene promoter polymorphisms with risk of asthenozoospermia (AZS) men. In addition, we performed association analysis between GRP78 promoter mutations and serum GRP78 level in asthenozoospermia.

Methods

The study population comprised 400 subjects with AZS patients and 400 healthy controls. We assessed GRP78 rs3216733, rs17840761, and rs17840762 polymorphisms by using Snapshot SNP genotyping assays; serum GRP78 level was measured by enzyme-linked immunosorbent assay (ELISA). Semen quality was assessed by computer-assisted semen analysis.

Results

We found that rs3216733 was associated with increased risk of AZS (Gd vs. dd: adjusted OR = 1.42, 95% CI, 1.06–1.93, P = 0.020; Gd/GG vs. dd: adjusted OR = 1.43, 95% CI, 1.08–1.91, P = 0.013; G vs. d adjusted OR = 1.26, 95% CI, 1.03–1.56, P = 0.027). The haplotype analyses showed the frequency of G-C-C haplotype was significantly higher in AZS (P = 0.026). The percentage of progressive motility sperm was lower in the asthenozoospermic men with Gd and Gd/GG genotypes than dd genotype (P = 0.003). Moreover, the serum GRP78 levels were significantly lower in rs3216733 Gd/GG genotypes compared with the dd genotype (P < 0.001).

Conclusion

Our findings suggest that rs3216733 Gd/GG genotypes contribute to poor sperm motility, probably by decreasing the level of GRP78.

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Funding

This work was supported by grants from the National Natural Science Foundation of China (No. 81560461), Research Key Laboratory of the Right River Valley Characteristics of Guangxi University (kfkt2017022).

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Authors and Affiliations

  1. Department of Reproduction and Genetics, The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China

    Haimei Qin, Xiaoxia Pang, Yuxiao Wei & Junli Wang

  2. Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China

    Haimei Qin, Rong Wang & Fenglian Yang

  3. Department of Laboratory Medicine, The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, China

    Rong Wang

Authors
  1. Haimei Qin
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  2. Rong Wang
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  3. Xiaoxia Pang
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  4. Yuxiao Wei
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  6. Junli Wang
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Corresponding author

Correspondence to Junli Wang.

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This study was approved by the ethics review board of the Affiliated Hospital of Youjiang Medical University for Nationalities, consistent with provisions of the Declaration of Helsinki. Voluntary written informed consent was obtained.

Conflict of interest

The authors declare that they have no competing interests.

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Qin, H., Wang, R., Pang, X. et al. Association of GRP78 promoter polymorphisms and serum GRP78 level with risk of asthenozoospermia. J Assist Reprod Genet 35, 2223–2231 (2018). https://doi.org/10.1007/s10815-018-1316-0

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  • DOI: https://doi.org/10.1007/s10815-018-1316-0

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