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The vascular endothelial growth factor (VEGF) +405 G/C polymorphism and its relationship with recurrent implantation failure in women in an IVF programme with ICSI

  • Genetics
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

Successful embryo implantation depends on trophoblast proliferation, migration and, lastly, invasion of the endometrium (to anchor the trophoblast to the uterus). This invasion is mediated by locally produced soluble factors. Of these, vascular endothelial growth factor (VEGF) is the best characterized regulator of angiogenesis. Here, we investigate the association between the VEGF + 405 C/G genotype and the recurrence of embryo implantation failure in women undergoing in vitro fertilization (IVF) program with intracytoplasmic sperm injection (ICSI).

Methods

Forty women with recurrent implantation failure defined by absence of pregnancy after transfer of more than 10 embryos and 131 women control, with at least one live birth after the transfer of fewer than 10 embryos were included. Genomic DNA was analysed with an allele-specific polymerase chain reaction and a Chi-2 test was used to compare the respective VEGF + 405 C/G genotype frequencies in cases and controls.

Results

The frequency of the VEGF +405C/C genotype was higher in women with recurrent implantation failure after ICSI-embryo transfer than in controls (17.5 % and 5.3 %, respectively, p = 0.01).

Conclusion

The VEGF +405 G/C polymorphism may influence embryo implantation and VEGF + 405 C/C genotype may predispose to recurrent implantation failure after ICSI-ET.

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Acknowledgment

We thanks AMMR-PSG and Schering-Ploug for their financial help.

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Authors and Affiliations

  1. Department of Reproductive Biology, Cytogenetics, Gynaecology and Obstetrics, Poissy Saint Germain Medical Centre, Poissy, 78303, France

    Radia Boudjenah, Denise Molina-Gomes, Robert Wainer, Jacqueline Selva & François Vialard

  2. EA 2493, Versailles Saint Quentin University, Versailles, 78035, France

    Radia Boudjenah, Denise Molina-Gomes, Robert Wainer, Philippe de Mazancourt, Jacqueline Selva & François Vialard

Authors
  1. Radia Boudjenah
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  2. Denise Molina-Gomes
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  3. Robert Wainer
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  4. Philippe de Mazancourt
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  5. Jacqueline Selva
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  6. François Vialard
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Correspondence to Radia Boudjenah or François Vialard.

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Capsule

Higher frequency of vascular endothelial growth factor (VEGF) +405 C/C polymorphism was observed in women after recurrent implantation failure in an IVF programme.

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Boudjenah, R., Molina-Gomes, D., Wainer, R. et al. The vascular endothelial growth factor (VEGF) +405 G/C polymorphism and its relationship with recurrent implantation failure in women in an IVF programme with ICSI. J Assist Reprod Genet 29, 1415–1420 (2012). https://doi.org/10.1007/s10815-012-9878-8

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  • DOI: https://doi.org/10.1007/s10815-012-9878-8

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