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Electropolymerization of hydroxyphenylacetic acid isomers and the development of a bioelectrode for the diagnosis of bacterial meningitis

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An Erratum to this article was published on 02 March 2017

Abstract

In this work, three polymeric films derived from hydroxyphenylacetic acid isomers were electropolymerized onto the surfaces of graphite electrodes through cyclic voltammetry. Analysis of electrochemical behaviors and electrical properties of the three polymeric films were investigated and their probably structures were explored by molecular modeling. The best functionalization strategy for the incorporation and retention of adenine and guanine nitrogenous bases was the poly(3-hydroxyphenylacetic acid). It was applied for the immobilization of a nucleotide probe specific to DNA of the bacteria Neisseria meningitidis operated in amperometric and impedimetric indirect modes. The simple protocol of electrochemical detection of DNA by the bioelectrode, developed in this work, could be further enhanced and applied in a low-cost and pain-less platform to diagnose human meningitis infection.

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Acknowledgments

The authors thank the Brazilian research agencies CNPq, FAPEMIG, and CAPES for their financial support and scholarships.

Author information

Authors and Affiliations

  1. Laboratório de Filmes Poliméricos e Nanotecnologia, Instituto de Química, Universidade Federal de Uberlândia, Uberlândia, MG, Brazil

    Luciano Pereira Rodrigues, Deusmaque Carneiro Ferreira & João M. Madurro

  2. Laboratório de Eletroquímica e Nanotecnologia, Instituto de Ciência e Tecnologia, Universidade Federal do Vale do Jequitinhonha e Mucuri, Diamantina, MG, Brazil

    Lucas Franco Ferreira

  3. Centro de Excelência em Bioinformática da Fiocruz-Minas, Belo Horizonte, MG, Brazil

    Sara Cuadros-Orellana & Guilherme Corrêa de Oliveira

  4. Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Uberlândia, MG, Brazil

    Ana G. Brito-Madurro

  5. Departamento de Física, Instituto de Ciências Exatas, Naturais e Educação, Universidade Federal do Triangulo Mineiro, Uberaba, MG, Brazil

    Ronaldo Junio de Oliveira

  6. Instituto de Ciências Biológicas e Naturais, Universidade Federal do Triangulo Mineiro, Uberaba, MG, Brazil

    Odonírio Abrahão Jr.

Authors
  1. Luciano Pereira Rodrigues
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  2. Deusmaque Carneiro Ferreira
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  3. Lucas Franco Ferreira
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  4. Sara Cuadros-Orellana
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  6. Ana G. Brito-Madurro
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  7. Ronaldo Junio de Oliveira
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  8. Odonírio Abrahão Jr.
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  9. João M. Madurro
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Correspondence to Odonírio Abrahão Jr..

Additional information

An erratum to this article is available at http://dx.doi.org/10.1007/s10800-017-1058-1.

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Rodrigues, L.P., Ferreira, D.C., Ferreira, L.F. et al. Electropolymerization of hydroxyphenylacetic acid isomers and the development of a bioelectrode for the diagnosis of bacterial meningitis. J Appl Electrochem 45, 1277–1287 (2015). https://doi.org/10.1007/s10800-015-0892-2

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