Skip to main content

Advertisement

SpringerLink
Log in

Relevance of Helicobacter pylori genotypes in gastric pathology and its association with plasma malondialdehyde and nitric oxide levels

  • Research Article
  • Published:
Inflammopharmacology Aims and scope Submit manuscript

Abstract

Persistent infection with Helicobacter pylori confers an increased risk of peptic ulceration and gastric adenocarcinoma. Reactive oxygen and nitrogen species play a crucial role in the progression from normal gastric mucosa to cancer. The aim of the present study was to investigate the plasma malondialdehyde and nitric oxide levels in H. pylori related gastroduodenal diseases and associate their levels with gastric pathology and genotypes of H. pylori. Malondialdehyde and nitric oxide levels in plasma samples of 250 subjects were spectrophotometrically determined. Subsequently, genotypic and histopathological assessment was performed in gastric biopsies obtained during endoscopy. The levels of MDA and NO exceeded in subjects infected with genotype-1 of Hp than those with other genotypes suggesting more precise interaction of highly virulent strains of Hp in eliciting severe tissue damage. In conclusion, the study demonstrates close relationship between the plasma malondialdehyde and nitric oxide levels, gastric histopathology and genotypes of H. pylori.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Arend A, Loime L, Roosaar P et al (2005) Helicobacter pylori substantially increases oxidative stress in indomethacin-exposed rat gastric mucosa. Medicina 42:343–347

    Google Scholar 

  • Bakan E, Taysi S, Polat MF et al (2002) Nitric oxide levels and lipid peroxidation in plasma of patients with gastric cancer. Jpn J Clin Oncol 32:162–166

    Article  PubMed  Google Scholar 

  • Biswas SK, Sodhi A, Paul S (2001) Regulation of nitric oxide production by murine peritoneal macrophages treated in vitro with chemokine monocyte chemoattractant protein 1. Nitric Oxide 5:566–579

    Article  CAS  PubMed  Google Scholar 

  • Dixon MF, Genta RM, Yardley JH et al (1996) Classification and grading of gastritis: the updated Sydney System. Am J Surg Pathol 20:1161–1181

    Article  CAS  PubMed  Google Scholar 

  • Everett SM, Singh R, Leuratti C et al (2001) Levels of malondialdehyde-deoxyguanosine in the gastric mucosa: relationship with lipid peroxidation, ascorbic acid, and Helicobacter pylori. Cancer Epidemiol Biomarkers Prev 10:369–376

    CAS  PubMed  Google Scholar 

  • Gallo O, Masini E, Morbidelli L et al (1998) Role of nitric oxide in angiogenesis and tumor progression in head and neck cancer. J Natl Cancer Inst 90:587–596

    Article  CAS  PubMed  Google Scholar 

  • Ghasemi A, Hedayati M, Biabani H (2007) Protein precipitation methods evaluated for determination of serum nitric oxide end products by the Griess assay. J Med Sci Res 2:29–32

    Google Scholar 

  • Hisatsune J, Yamasaki E, Nakayama M et al (2007) Helicobacter pylori VacA enhances prostaglandin E2 production through induction of cyclooxygenase 2 expression via a p38 mitogen-activated protein kinase/activating transcription factor 2 cascade in AZ-521 cells. Infect Immun 75:4472–4481

    Article  CAS  PubMed  Google Scholar 

  • Ilhan N, Ilhan N, Ilhan Y et al (2004) C-reactive protein, procalcitonin, interleukin-6, vascular endothelial growth factor and oxidative metabolites in diagnosis of infection and staging in patients with gastric cancer. World J Gastroenterol 10:1115–11120

    CAS  PubMed  Google Scholar 

  • Karita M, Matsumoto S, Kamei T et al (2003) The size of cagA based on repeat sequence has the responsibility of the location of Helicobacter pylori in the gastric mucus and the degree of gastric mucosal inflammation. Microbiol Immunol 47:619–630

    CAS  PubMed  Google Scholar 

  • Kim PK, Zamora R, Petrosko P et al (2001) The regulatory role of nitric oxide in apoptosis. Int Immunopharmacol 1:1421–1441

    Article  CAS  PubMed  Google Scholar 

  • Kwiecien S, Brzozowski T, Konturek PC et al (2004) Gastroprotection by pentoxyfilline against stress induced gastric damage. Role of lipid peroxidation, antioxidizing enzymes and proinflammatory cytokines. J Physiol Pharmacol 55:337–355

    CAS  PubMed  Google Scholar 

  • Ladeira MS, Bueno RC, Dos Santos BF et al (2008) Relationship among oxidative DNA damage, gastric mucosal density and the relevance of cagA, vacA and iceA genotypes of Helicobacter pylori. Dig Dis Sci 53:248–255

    Article  CAS  PubMed  Google Scholar 

  • Miranda KM, Espey MG, Wink DA (2001) A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. Nitric Oxide 5:62–71

    Article  CAS  PubMed  Google Scholar 

  • Nielsen H, Andersen LP (1992) Activation of human phagocyte oxidative metabolism by Helicobacter pylori. Gastroenterology 103:1747–1753

    CAS  PubMed  Google Scholar 

  • Nogueira C, Figueiredo C, Carneiro F et al (2001) Helicobacter pylori genotypes may determine gastric histopathology. Am J Pathol 158:647–654

    CAS  PubMed  Google Scholar 

  • Peleteiro B, Lunet N, Figueiredo C et al (2007) Smoking, Helicobacter pylori virulence, and type of intestinal metaplasia in Portuguese males. Cancer Epidemiol Biomarkers Prev 16:322–326

    Article  CAS  PubMed  Google Scholar 

  • Rajnakova A, Goh PM, Chan ST et al (1997) Expression of differential nitric oxide synthase isoforms in human normal gastric mucosa and gastric cancer tissue. Carcinogenesis 18:1841–1845

    Article  CAS  PubMed  Google Scholar 

  • Ruetten H, Thiemermann C, Perretti M (1999) Upregulation of ICAM-1 expression on J774.2 macrophages by endotoxin involves activation of NF-kappaB but not protein tyrosine kinase: comparison to induction of iNOS. Mediators Inflamm 8:77–84

    Article  CAS  PubMed  Google Scholar 

  • Suematsu M, Suzuki H, Delano FA et al (2002) The inflammatory aspect of the microcirculation in hypertension: oxidative stress, leukocytes/endothelial interaction, apoptosis. Microcirculation 9:259–276

    CAS  PubMed  Google Scholar 

  • Tiwari SK, Khan AA, Manoj G et al (2007) A simple multiplex PCR assay for diagnosing virulent Helicobacter pylori infection in human gastric biopsy specimens from subjects with gastric carcinoma and other gastro-duodenal diseases. J Appl Microbiol 103:2353–2360

    Article  CAS  PubMed  Google Scholar 

  • Tiwari SK, Manoj G, Vasanth Kumar G et al (2008) Prognostic significance of genotyping Helicobacter pylori infection in patients with gastric cancer in younger age groups. Postgrad Med J 84:193–197

    Article  CAS  PubMed  Google Scholar 

  • Wang YF, Guo CL, Zhao LZ et al (2005) Effect of Helicobacter pylori on gastric mucosal pathologic changes and level of nitric oxide synthase. World J Gastroenterol 32:5029–5031

    Google Scholar 

  • Yagi K (1976) A simple fluorometric assay for lipoperoxide in blood plasma. Biochem Med 15:212–216

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This study was not funded by any intra- or extramural research grants. The authors would like to thank Mr. Ashok Kumar, Project fellow, National Institute of Nutrition, India for his technical assistance during the course of study.

Conflict of interest statement

The authors declare no conflict of interest.

Author information

Authors and Affiliations

  1. Centre for Liver Research and Diagnostics, Deccan College of Medical Sciences, Kanchanbagh, Hyderabad, 500 058, Andhra Pradesh, India

    Santosh K. Tiwari, G. Manoj, Vishwas Sharma, G. Sivaram, R. Saikant, Avinash Bardia, Varun K. Sharma, Aleem A. Khan, M. Aejaz Habeeb, C. M. Habibullah & Amrita Nandan

  2. Department of Pathology, Deccan College of Medical Sciences, Kanchanbagh, Hyderabad, 500 058, Andhra Pradesh, India

    Zakia Abid

  3. Department of Physiology, Deccan College of Medical Sciences, Kanchanbagh, Hyderabad, 500 058, Andhra Pradesh, India

    B. Santhosh Kumar

Authors
  1. Santosh K. Tiwari
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. G. Manoj
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Vishwas Sharma
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. G. Sivaram
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. R. Saikant
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Avinash Bardia
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Varun K. Sharma
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Zakia Abid
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Aleem A. Khan
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. M. Aejaz Habeeb
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. C. M. Habibullah
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. B. Santhosh Kumar
    View author publications

    You can also search for this author in PubMed Google Scholar

  13. Amrita Nandan
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Aleem A. Khan.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tiwari, S.K., Manoj, G., Sharma, V. et al. Relevance of Helicobacter pylori genotypes in gastric pathology and its association with plasma malondialdehyde and nitric oxide levels. Inflammopharmacol 18, 59–64 (2010). https://doi.org/10.1007/s10787-010-0031-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10787-010-0031-y

Keywords

Search

Navigation