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Dynamics of biochemical and morphophysiological changes during zygotic embryogenesis in Acca sellowiana (Berg.) Burr.

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Abstract

Acca sellowiana (Berg.) Burr. is a native Myrtaceae from southern Brazil and Uruguay, now the subject of a domestication and breeding program. Biotechnological tools have been used to assist in this program. The establishment of a reliable protocol of somatic embryogenesis has been pursued, with a view to capturing and fixing genetic gains. The rationale behind this work relies on the fact that deepening comprehension of the general metabolism of zygotic embryogenesis may certainly improve the protocol for somatic embryogenesis. Thus, in the present work we studied the accumulation of protein, total sugars, starch, amino acids, polyamines (PAs), IAA and ABA, in different stages of A. sellowiana zygotic embryogenesis. Starch is the predominant storage compound during zygotic embryo development. Increased synthesis of amino acids in the cotyledonary stage, mainly of asparagine, was observed throughout development. Total free PAs showed increased synthesis, whereas total conjugated PAs were mainly observed in the early developmental stages. IAA decreased and ABA increased with the progression from early to late embryogenesis. Besides providing basic information on the morphophysiological and biochemical changes of zygotic embryogenesis, the results here obtained may provide adequate strategies towards the modulation of somatic embryogenesis in this species as well as in other woody angiosperms.

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Authors and Affiliations

  1. Graduate Program in Plant Genetic Resources, Plant Developmental Physiology and Genetics Laboratory, Federal University of Santa Catarina, Florianópolis, SC, 88040-900, Brazil

    Gabriela Claudia Cangahuala-Inocente, Clarissa Alves Caprestano & Miguel Pedro Guerra

  2. Biotechnology Laboratory, CBB, State University of North Fluminense (UENF), Av. Alberto Lamego, 2000, Campos dos Goytacazes, Rio de Janeiro, 28013-600, Brazil

    Vanildo Silveira

  3. Epagri, São Joaquim Experimental Station, São Joaquim, SC, 88600-000, Brazil

    Jean Pierre Henry Joseph Ducroquet

  4. Laboratory of Plant Cell Biology, Department of Botany, IB-University of São Paulo, São Paulo, SP, 05422-970, Brazil

    Eny Iochevet Segal Floh

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  1. Gabriela Claudia Cangahuala-Inocente
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  2. Vanildo Silveira
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  3. Clarissa Alves Caprestano
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  4. Jean Pierre Henry Joseph Ducroquet
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  5. Eny Iochevet Segal Floh
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  6. Miguel Pedro Guerra
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Correspondence to Miguel Pedro Guerra.

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Cangahuala-Inocente, G.C., Silveira, V., Caprestano, C.A. et al. Dynamics of biochemical and morphophysiological changes during zygotic embryogenesis in Acca sellowiana (Berg.) Burr.. Plant Growth Regul 59, 103–115 (2009). https://doi.org/10.1007/s10725-009-9393-9

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