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Molecular characterization of accessions of a rare genetic resource: sugary cassava (Manihot esculenta Crantz) from Brazilian Amazon

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Abstract

Sugary cassava (Manihot esculenta) represents a type of cassava that accumulates sugar in its root due to a deficiency to convert sugar into starch. This type of cassava is usually found in the Brazilian Amazon. The genetic variability of a group of 39 accessions of sugary cassavas sampled in the States of Amazonas and Pará in Brazil and conserved in two germplasm banks was investigated with eleven microsatellite loci. All eleven loci were polymorphic, with 5.91 alleles per locus. Analyses indicated that 14 of the 39 accessions had the same multiloci profile and represented four genotypes, with 4.03 × 10−7 of probability of identity. Duplicates were discarded and 29 genotypes were used in the genetic analyses for groups. Structure analysis divided the 29 genotypes into two groups (K = 2), where group 1 contained only genotypes sampled in the northeast of the State of Pará and group 2 contained genotypes sampled in the State of Amazonas, west of Pará and one genotype from the northeast of Pará. AMOVA indicated high differentiation among the two States (ΦST = 0.374**) and among the two groups detected by Structure (ΦST = 0.45**). Genetic parameters indicated higher genetic diversity among genotypes of group 2. Results showed that there are two genetic groups of sugary cassavas conserved in the germplasm banks of Brazil and they must be investigated in order to identify different sources of mutations associated with starch conversion route.

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Acknowledgments

The authors thank National Counsel of Technological and Scientific Development (CNPq) Process No. 407991/2013-1, to Amazon Research Foundation (FAPESPA), Process No. 181/2009 and Brazilian Agricultural Research Corporation (Embrapa), Process No. 02.14.00.018.00.00 for financial support and scholarship grants.

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Authors and Affiliations

  1. Embrapa (Brazilian Agricultural Research Corporation) Amazônia Oriental, Trav. Dr. Enéas Pinheiro, s/n°, Marco, Caixa Postal 48, Belém, PA, 66095-100, Brazil

    Elisa Ferreira Moura, Mônika Fecury Moura, João Tomé de Farias Neto & José Edson Sampaio

  2. Embrapa Cocais, Av. São Luís Rei de França, nº 4, Quadra 11, Conjunto Eldorado, Bairro Turu, São Luís, MA, 65065-470, Brazil

    Nelcimar Reis Sousa

  3. Embrapa Amazônia Ocidental, Rodovia AM-010, Km 29, Caixa Postal 319, Manaus, AM, 69010-970, Brazil

    Miguel Costa Dias

  4. Embrapa Roraima, Rodovia BR 174, Km 8, Distrito Industrial, Caixa Postal 133, Boa Vista, RR, 69301-970, Brazil

    Everton Diel Souza

Authors
  1. Elisa Ferreira Moura
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  2. Nelcimar Reis Sousa
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  3. Mônika Fecury Moura
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  4. Miguel Costa Dias
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  5. Everton Diel Souza
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  6. João Tomé de Farias Neto
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  7. José Edson Sampaio
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Correspondence to Elisa Ferreira Moura.

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Moura, E.F., Sousa, N.R., Moura, M.F. et al. Molecular characterization of accessions of a rare genetic resource: sugary cassava (Manihot esculenta Crantz) from Brazilian Amazon. Genet Resour Crop Evol 63, 583–593 (2016). https://doi.org/10.1007/s10722-016-0378-z

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