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Synthesis and biological roles of O-glycans in insects

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Abstract

Protein O-glycosylation is the attachment of carbohydrate structures to the oxygen atom in the hydroxyl group of Serine and Threonine residues. This post-translational modification is commonly found on the majority of proteins trafficking through the secretory pathway and is reported to influence protein characteristics such as folding, secretion, stability, solubility, oligomerization and intracellular localization. In addition, O-glycosylation is essential for cell-cell interactions, protein-protein interactions and many biological processes, such as stress response, immunization, phosphorylation, ubiquitination, cell division, metabolism and cell signaling. The availability of sequenced genomes and genetic tools to create mutants with clear phenotypes makes insects an interesting model system to study O-glycosylation. In this review, we provide an overview of the current knowledge of O-glycosylation, mainly obtained from the model organism Drosophila melanogaster, with a focus on the synthesis and biological roles of the common O-glycans in insects.

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Acknowledgements

This work is supported by the Special Research Fund from Ghent University (Belgium). W.L. is a recipient of a Chinese Scholarship Council (CSC) scholarship.

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Authors and Affiliations

  1. Laboratory of Agrozoology, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium

    Weidong Li, Kristof De Schutter & Guy Smagghe

  2. Laboratory of Biochemistry and Glycobiology, Department of Biotechnology, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium

    Weidong Li & Els J. M. Van Damme

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Li, W., De Schutter, K., Van Damme, E.J.M. et al. Synthesis and biological roles of O-glycans in insects. Glycoconj J 37, 47–56 (2020). https://doi.org/10.1007/s10719-019-09867-1

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