Abstract
Plant hormone abscisic acid (ABA) plays an important role in plant growth, development and response to biotic / abiotic stressors. Thus, it is necessary to investigate the crucial genes associated with ABA synthesis. Currently, the carotenoid cleavage oxygenases (CCOs) family that function as the key step for ABA synthesis are not well understood in banana. In this study, 13 MaCCO genes and 12 MbCCO genes, divided into NCED subgroup and CCD subgroup, were identified from the banana genome, and their evolutionary relationship, protein motifs, and gene structures were also determined. Transcriptomic analysis suggested the involvement of CCO genes in banana development, ripening, and response to abiotic and biotic stressors, and homologous gene pairs showed homoeologue expression bias in the A or B subgenome. Our results identified MaNCED3A, MaCCD1, and MbNCED3B as the genes with the highest expression during fruit development and ripening. MaNCED5 / MbNCED5 and MaNCED9A might respond to abiotic stress, and MaNCED3A, 3B, 6 A, 9 A, and MbNCED9A showed transcriptional changes that could be a response to Foc4 infection. These findings may contribute to the characterization of key enzymes involved in ABA biosynthesis, as well as to identify potential targets for the genetic improvement of banana.
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Abbreviations
- ABA:
-
abscisic acid
- CCD:
-
carotenoid cleavage dioxygenase
- CCO:
-
carotenoid cleavage oxygenases
- DPH:
-
day postharvest
- NCED:
-
nine-cis epoxide carotenoid dioxygenase
- Foc4:
-
Fusarium oxysporum race 4
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Funding
This research was supported by the National Key Research and Development Program of China (2019YFD1000204), Hainan Provincial Natural Science Foundation of China (2019RC295), 2020 Research Program of Sanya Yazhou Bay Science and Technology City (SKJC-2020-02-002), National Natural Science Foundation of China (31901537), Guangdong Basic and Applied Basic Research Foundation (2021A1515011236), Research Platform Project of Education Department of Guangdong Province (2022ZDZX4047), and Open Fund of the Guangdong Provincial Key Laboratory of Utilization and Conservation of Food and Medicinal Resources in Northern Region.
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Conceptualization, WH; Methodology and software, YW and ZNX; Validation, LWZ; Formal analysis, XXY, WWT, MHZ, JC, and MYL; Writing original draft preparation, LWM, LWZ, and JZ; Supervision, SS, JZ, and WH. All authors have read and agreed to the published version of the manuscript. We also thank Dandan Li for editorial assistance.
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Zeng, L., Zeng, L., Wang, Y. et al. Identification and expression of the CCO family during development, ripening and stress response in banana. Genetica 151, 87–96 (2023). https://doi.org/10.1007/s10709-023-00178-w
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DOI: https://doi.org/10.1007/s10709-023-00178-w