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Nutrition and water temperature regulate the expression of heat-shock proteins in golden pompano larvae (Trachinotus ovata, Limmaeus 1758)

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Abstract

Understanding fish larval development is of a great interest for aquaculture production efficiency. Identifying possible indicators of fish larvae stress could improve the production and limit the mortality rate that larval stage is subjected to. Heat-shock proteins (HSPs) and heat-shock factors (HSFs) are well known as indicators of response to many kinds of stressor (e.g., environmental, morphological, or pathological changes). In this study, golden pompano larvae were raised at different temperatures (23 °C, 26 °C, and 29 °C), as well as three different diets (Artemia nauplii unenriched, Artemia nauplii enriched with Nannochloropsis sp., and Artemia nauplii enriched with Algamac 3080), and the expression of HSP60, HSP70, HSF1, HSP2, and GRP94 were monitored. While stress genes were widely expressed in the larval tissues, HSP60 and HSP70 were principally from the gills and heart; HSF1 principally from the muscle, brain, and heart; and GRP94 principally from the head kidney and spleen. Golden pompano larvae were found to be more sensitive to thermal changes at later larval stage, and 29 °C was showed to likely be the best condition for golden pompano larval development. Nannochloropsis sp.–enriched Artemia nauplii treatment was found to be the most appropriate feed type with moderate relative expressions of HSP60, HSP70, HSF1, HSF2, and GRP94.

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Funding

This project was supported by the National Natural Science Foundation of China (31502186), Pearl River S&T Nova Program of Guangzhou (201610010166), and Ministry of Human Resources and Social Security of China (2016 High-level Overseas Researcher Come Back to Work Funds).

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Authors and Affiliations

  1. Tropical Aquaculture Research and Development Center, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Sanya, 572018, China

    Laetitia Allais, Chao Zhao, Jing Hu, Lihua Qiu & Zhenhua Ma

  2. College of Science and Engineering, Flinders University, GPO Box 2100, Adelaide, SA, 5001, Australia

    Laetitia Allais, Jian G. Qin & Zhenhua Ma

  3. Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture, Guangzhou, 510300, People’s Republic of China

    Chao Zhao, Jing Hu & Zhenhua Ma

  4. College of Life Science, Longyan University, Longyan, 364012, Fujian, China

    Mingjun Fu

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  1. Laetitia Allais
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  2. Chao Zhao
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  3. Mingjun Fu
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  4. Jing Hu
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  5. Jian G. Qin
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  6. Lihua Qiu
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  7. Zhenhua Ma
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Correspondence to Zhenhua Ma.

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The Trachinotus ovatus is not endangered or protected species, and there is no requirement for permission to perform experiments involving this species in China.

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Allais, L., Zhao, C., Fu, M. et al. Nutrition and water temperature regulate the expression of heat-shock proteins in golden pompano larvae (Trachinotus ovata, Limmaeus 1758). Fish Physiol Biochem 45, 485–497 (2019). https://doi.org/10.1007/s10695-018-0578-x

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