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Effects of acute hyperglycemia stress on plasma glucose, glycogen content, and expressions of glycogen synthase and phosphorylase in hybrid grouper (Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂)

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Abstract

In the present study, the hybrid grouper (Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂), a typical carnivorous fish, was chosen as a model to investigate the regulation of glycogen metabolism owning to its characteristic of glucose intolerance. The variation of plasma glucose concentration, glycogen content, and expressions of glycogen metabolism-related genes under acute hyperglycemia stress were measured. Following glucose administration, plasma glucose concentration increased immediately, and the glucose level remained elevated for at least 12 h. The prolonged glucose clearance and hyperglycemia revealed glucose intolerance of this fish species. Meanwhile, the glycogen content in both liver and muscle changed significantly during the clearance of plasma glucose. However, the peak value of hepatic glycogen (1 and 12 h post injection) appeared much earlier than muscle (3 and 24 h post injection). To investigate the regulation of glycogen metabolism from molecular aspect, the complete coding sequence (CDS) of glycogen synthase (GS) and glycogen phosphorylase (GP) in both liver and muscle types were obtained, encoding a polypeptide of 704, 711, 853, and 842 amino acid residues, respectively. The results of gene expression analysis revealed that the expression of liver type and muscle type GS was significantly higher than other time points at 12 and 24 h post glucose injection, respectively. Meanwhile, the highest expressions of GP in both liver and muscle types occurred at 24 h post glucose injection. The response of GS and GP to glucose load may account for the variation of glycogen content at the transcriptional level to some extent.

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Acknowledgements

The work was supported by China Postdoctoral Science Foundation (2016M601566); the China-ASEAN Maritime Cooperation Fund, China-ASEAN Center for Joint Research and Promotion of Marine Aquaculture Technology (DF); Shanghai Agriculture Applied Technology Development Program, China (Grant No.G20130508); Open fund of Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology (2016LMFS-B17); and Key Laboratory of Mariculture of Ministry of Education, Ocean University of China (KLM2017003).

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Authors and Affiliations

  1. National Demonstration Center on Experiment Teaching of Fisheries Science, Shanghai Ocean University, Shanghai, 201306, China

    Songlin Li, Chunyan Sang, Jiacan Zhang, Naisong Chen, Ziqiang Li, Pengfei Jin & Xuxiong Huang

  2. Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, 1 Wenhai Road, Qingdao, 266237, China

    Songlin Li

  3. Research Centre of the Agriculture Ministry on Environmental Ecology and Fish Nutrition, Shanghai Ocean University, Shanghai, 20136, China

    Naisong Chen

  4. Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai Ocean University, Shanghai, 201306, China

    Naisong Chen

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  1. Songlin Li
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  2. Chunyan Sang
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  3. Jiacan Zhang
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  4. Naisong Chen
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Correspondence to Naisong Chen.

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Li, S., Sang, C., Zhang, J. et al. Effects of acute hyperglycemia stress on plasma glucose, glycogen content, and expressions of glycogen synthase and phosphorylase in hybrid grouper (Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂). Fish Physiol Biochem 44, 1185–1196 (2018). https://doi.org/10.1007/s10695-018-0508-y

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