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Identification of QTL underlying soluble pigment content in soybean stems related to resistance to soybean white mold (Sclerotinia sclerotiorum)

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Abstract

The frequency of soybean white mold (Sclerotinia sclerotiorum, SWM) outbreaks has increased since 1978, and this disease is currently considered to be the second most important cause of soybean yield loss worldwide. We have studied SWM in stems of soybean cultivar Maple Arrow, which shows partial resistance to SWM, in an attempt to identify the quantitative trait loci (QTLs) underlying soluble pigment(s) content, which is associated with SWM resistance. A SWM-susceptible cultivar, Hefeng 25, was crossed with Maple Arrow, and 149 F5:6 recombinant inbred lines were subsequently advanced through single-seed-descent. A total of 109 simple sequence repeat (SSR) markers were used to construct a genetic linkage map. Three QTLs for soluble pigment content in soybean stems associated with the resistance to SWM, namely, Qsp-1 (Satt502-Sat_159), Qsp-2 (Sat_156-Satt251), and Qsp-3 (Satt525-Satt233), were identified in 2007 and 2008 and located onto linkage groups D1a+q, B1 and A2, respectively. The phenotypic variation (R 2) explained by these QTLs ranged from 6.29 to 15.37%. These three QTLs were not significantly related to known QTLs associated with escape resistant mechanisms. The use of these QTLs in marker-assisted selection may contribute to improved soybean resistance to SWM.

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Acknowledgments

This study was conducted in the Key Laboratory of Soybean Biology of Chinese Education Ministry and financially supported by National 863 Projects (Contract No. 2006AA10Z1F1 and 2006AA100104-4), 948 project of Agricultural ministry of China (Contract No. 2006-G5) and National International Cooperation Project.

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  1. Chinese Education Ministry’s Key Laboratory of Soybean Biology (Soybean Research Institute), Northeast Agricultural University, 150030, Harbin, China

    Dongmei Li, Mingming Sun, Yingpeng Han, Weili Teng & Wenbin Li

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  1. Dongmei Li
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  2. Mingming Sun
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  3. Yingpeng Han
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  4. Weili Teng
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  5. Wenbin Li
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Correspondence to Wenbin Li.

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Dongmei Li, Mingming Sun and Yingpeng Han have made equal contributions to this work.

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Li, D., Sun, M., Han, Y. et al. Identification of QTL underlying soluble pigment content in soybean stems related to resistance to soybean white mold (Sclerotinia sclerotiorum). Euphytica 172, 49–57 (2010). https://doi.org/10.1007/s10681-009-0036-z

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