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Utilization of rhizospheric Streptomyces for biological control of Rigidoporus sp. causing white root disease in rubber tree

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Abstract

White root disease causing by Rigidoporus sp. is a severe problem that decreases latex productivity and can even cause mortality of rubber trees. With the aim to control biologically this disease, antifungal actinobacteria were isolated from rhizospheric soils of some medicinal plants cultivated in Thailand. Among all isolated actinobacteria, an isolate TM32 exhibited distinctive antagonistic activity against the fungus. Cell-free culture broth of the isolate showed median effective dose (ED50) of 2.61 ml l−1 (equal to 1.19 g l−1 of metalaxyl). The isolate was also able to solubilize phosphate and to produce chitinase (enzyme activity = 0.093 ± 0.004 U ml−1), siderophore (average clear zone, 11.75 ± 0.96 mm) and indole-3-acetic acid (54.00 ± 1.00 μg ml−1). Application of biocontrol starters produced by this isolate in nursery stage of rubber trees farming showed greater suppression of the disease than direct use of its biocontrol agents. The biocontrol starters also enhanced growth of the rubber trees by increasing their heights. This might be due to the persistent growth of the isolate by using the organic substrate remaining in soil, which could later antagonize fungal pathogens through colonization at the rhizosphere and immunization of the rubber trees. The isolate revealed phylogenetically related to Streptomyces sioyaensis supported by 99 % similarity of 16S rRNA gene sequences. We concluded that application of the biocontrol starters produced by this Streptomyces isolate would be an alternative approach for sustainable control of soil-borne fungal invasion in long-term rubber tree farming.

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References

  • Atlas, R. M. (1946). Handbook of microbiological media (2nd ed.). Florida: CRC Press.

    Google Scholar 

  • Bano, N., & Musarrat, J. (2003). Characterization of a new Pseudomonas aeruginosa strain NJ-15 as a potential biocontrol agent. Current Microbiology, 46, 324–328.

    Article  CAS  PubMed  Google Scholar 

  • Business Report Thailand. (2011). Thai Rubber Industry Success. Business Report Thailand, Issue 6. http://www.businessreportthailand.com/thai-rubber-industry-success-12632). Accessed 8 Dec 2011.

  • Cattelan, A. J., Hartel, P. G., & Fuhrmann, J. J. (1999). Screening for plant growth-promoting rhizobacteria to promote early soybean growth. Soil Science Society of American Journal, 63, 1670–1680.

    Article  CAS  Google Scholar 

  • Evueh, G. A., & Ogbebor, N. O. (2008). Use of phylloplane fungi as biocontrol agent against Colletotrichum leaf disease of rubber (Hevea brasiliensis Muell. Arg.). African Journal of Biotechnology, 7, 2569–2572.

    Google Scholar 

  • Evueh, A. G., Okhuoya, J. A., Osemwegie, O. O., Attitalla, I. H., & Ogbebor, O. N. (2011). Evaluation of phylloplane fungi as biocontrol agent of Corynespora leaf fall disease of rubber (Hevea brasiliensis Muell. Arg.). World Journal of Fungal and Plant Biology, 2, 01–05.

    Google Scholar 

  • Hasegawa, T., Takizawa, M., & Tanida, S. (1983). A rapid analysis for chemical grouping of aerobic actinomycetes. Journal of General and Applied Microbiology, 29, 319–322.

    Article  CAS  Google Scholar 

  • Hayakawa, M., & Nonomura, H. (1987). Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. Journal of Fermentation Technology, 65, 501–509.

    Article  CAS  Google Scholar 

  • Hayakawa, M., Yoshida, Y., & Iimura, Y. (2004). Selective isolation of bioactive soil actinomycetes belonging to the Streptomyces violaceusniger phenotypic cluster. Journal of Applied Microbiology, 96, 973–981.

    Article  CAS  PubMed  Google Scholar 

  • Hong, T. Y., Cheng, C. W., Huang, J. W., & Meng, M. (2002). Isolation and biochemical characterization of an endo-1,3-β-glucanase from Streptomyces sioyaensis containing a C-terminal family 6 carbohydrate-binding module that binds to 1,3-β-glucan. Microbiology, 148, 1151–1159.

    CAS  PubMed  Google Scholar 

  • Hong, T. Y., Hsiao, Y. Y., Meng, M., & Li, T. T. (2008). The 1.5 Å structure of endo-1,3-β-glucanase from Streptomyces sioyaensis: evolution of the active-site structure for 1,3-β-glucan-binding specificity and hydrolysis. Acta Crystallographica Section D Biological Crystallography, 64, 964–970.

    Article  CAS  Google Scholar 

  • Hopwood, D. A., Bibb, M. J., Chater, K. F., Kieser, T., Bruton, C. J., Kieser, H. M., Lydiate, D. J., Smith, C. P., Ward, J. M., & Schrempf, H. (1985). Genetic Manipulation of Streptomyces. A Laboratory Manual. John Innes Foundation Norwich, UK.

  • Idwan, S. L., Iraqi, H. A. I., Le Febvre, G., Kiffer, E., & Botton, B. (1992). Screening of some basidiomycetes for biological control of Rigidoporus lignosus, a parasite of rubber tree, Hevea brasiliensis. Mycological Research, 96, 621–625.

    Article  Google Scholar 

  • Jayasinghe, B. A. T. D., & Parkinson, D. (2007). Actinomycetes as antagonists of litter decomposer fungi. Applied Soil Ecology, 38, 109–118.

    Article  Google Scholar 

  • Jayasuriya, K. E., & Thennakoon, B. I. (2007). Biological control of Rigidoporus microporus, the cause of white root disease in rubber. Ceylon Journal of Science (Biological Science), 36, 9–16.

    Google Scholar 

  • Kaewchai, S., & Soytong, K. (2010). Application of biofungicides against Rigidoporus microporus causing white root disease of rubber trees. Journal of Agricultural Science and Technology, 6, 349–363.

    Google Scholar 

  • Kaewchai, S., Wang, H. K., Lin, F. C., Hyde, K. D., & Soytong, K. (2009). Genetic variation among isolates of Rigidoporus microporus causing white root disease of rubber trees in Southern Thailand revealed by ISSR markers and pathogenicity. African Journal of Microbiology Research, 3, 641–648.

    CAS  Google Scholar 

  • Lane, D. J. (1991). 16S/23S rRNA sequencing. In E. Stackebrandt & M. Goodfellow (Eds.), Nucleic acid techniques in bacterial systematics (pp. 115–148). Chichester: Wiley.

    Google Scholar 

  • Lechevalier, M. P., & Lechevalier, H. (1970). Chemical composition as a criterion in the classification of aerobic actinomycetes. International Journal of Systematic and Evolutionary Microbiology, 20, 435–443.

    CAS  Google Scholar 

  • Loqman, S., Barka, E. A., Clement, C., & Ouhdouch, Y. (2009). Antagonistic actinomycetes from Moroccan soil to control the grapevine gray mold. World Journal of Microbiology and Biotechnology, 25, 81–91.

    Article  Google Scholar 

  • Malviya, M. K., Pandey, A., Trivedi, P., Gupta, G., & Kumar, B. (2009). Chitinolytic activity of cold tolerant antagonistic species of Streptomyces isolated from glacial sites of Indian Himalaya. Current Microbiology, 59, 502–508.

    Article  CAS  PubMed  Google Scholar 

  • Nandris, D., Nicole, M., & Geiger, J. P. (1987a). Root rot diseases of rubber trees. Plant Disease, 71, 298–306.

    Article  Google Scholar 

  • Nandris, D., Nicole, M., & Geiger, J. P. (1987b). Variation in virulence among Rigidoporus lignosus and Phellinus noxius isolates from West Africa. Sonderdruck aus European Journal of Forest Pathology, 17, 271–281.

    Article  Google Scholar 

  • Nautiyal, C. S. (1999). An efficient microbiological growth medium for screening phosphate solubilizing microorganisms. FEMS Microbiology Letter, 170, 265–270.

    Article  CAS  Google Scholar 

  • Nicole, M., & Benhamou, N. (1991). Cytochemical aspects of cellulose breakdown during the infection process of rubber tree roots infected by Rigidoponus lignosus. Phytopathology, 81, 1412–1420.

    Article  Google Scholar 

  • Ogbebor, N. O., Adekunle, A. T., Eghafona, N. O., & Ogboghodo, A. I. (2010). Ganoderma psuedoferreum: Biological control possibilities with microorganisms isolated from soils of rubber plantations in Nigeria. African Journal of General Agriculture, 6, 301–305.

    Google Scholar 

  • Pandey, A., & Palni, L. M. S. (2007). The rhizosphere effect in trees of the Indian Central Himalaya with special reference to altitude. Applied Ecology and Environmental Research, 5, 93–102.

    Article  Google Scholar 

  • Para, G., & Ristaino, J. (1998). Insensitivity to Ridomil Gold (mefenoxam) found among field isolates of Phytophthora capsici causing Phytophthora blight on bell pepper in North Carolina and New Jersey. Plant Disease, 82, 711–711.

    Article  Google Scholar 

  • Rose, K., & Steinbüchel, A. (2005). Biodegradation of natural rubber and related compounds: recent insights into a hardly understood catabolic capability of microorganisms. Applied and Environmental Microbiology, 71, 2803–2812.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Saitou, N., & Nei, M. (1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 4, 406–425.

    CAS  PubMed  Google Scholar 

  • Schwyn, B., & Neilands, J. B. (1987). Universal chemical assay for the detection and determination of siderophores. Analytical Biochemistry, 160, 47–56.

    Article  CAS  PubMed  Google Scholar 

  • Tamura, T., Hayakawa, M., & Hatano, K. (1997). A new genus of the order Actinomycetales, Spirilliplanes gen. nov., with description of Spirilliplanes yamanashiensis sp. nov. International Journal of Systematic Bacteriology, 47, 97–102.

    Article  CAS  PubMed  Google Scholar 

  • Tamura, K., Dudley, J., Nei, M., & Kumar, S. (2007). MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution, 24, 1596–1599.

    Article  CAS  PubMed  Google Scholar 

  • Teather, R. M., & Wood, P. J. (1982). Use of Congo red polysaccharide interactions complex formation between Congo red and polysaccharide in detection and assay of polysaccharide hydrolases. Methods in Enzymology, 160, 59–74.

    Google Scholar 

  • Tewtrakul, S., & Subhadhirasakul, S. (2007). Anti-allergic activity of some selected plants in the Zingiberaceae family. Journal of Ethnopharmacology, 109, 535–538.

    Article  PubMed  Google Scholar 

  • Tokala, R. K., Strap, J. L., Jung, C. M., Crawford, D. L., Salove, M. H., Deobald, L. A., Bailey, J. F., & Morra, M. J. (2002). Novel plant-microbe rhizosphere interaction involving Streptomyces lydicus WYEC108 and the pea plant (Pisum sativum). Applied and Environmental Microbiology, 68, 2161–2171.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Vasconcellos, R. L. F., & Cardoso, E. J. B. N. (2009). Rhizospheric streptomycetes as potential biocontrol agents of Fusarium and Armillaria pine rot and as PGPR for Pinus taeda. BioControl, 54, 807–816.

    Article  Google Scholar 

  • Wilhelm, S. (1973). Principles of biological control of soil-borne plant diseases. Soil Biology and Biochemistry, 5, 729–737.

    Article  Google Scholar 

  • Yang, S., & Huang, C. I. (1994). Protease production by amylolytic fungi in solid state fermentation. Journal of the Chinese Agricultural Chemical Society, 32, 589–601.

    CAS  Google Scholar 

  • Yu, T., Wang, L., Yin, Y., Wang, Y., & Zheng, X. (2008). Effect of chitin on the antagonistic activity of Cryptococcus laurentii against Penicillium expansum in pear fruit. International Journal of Food Microbiology, 122, 44–48.

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgment

This research was fully funded by the Thailand Research Fund (TRF) under the Small Projects on Rubber (SPR) with a grant no. RDG5250036 and RDG5350024. We thank Prof. Dr. Gavin Reynolds, Biomedical Science Research centre, Sheffield Hallam University, UK for his kind assistance on improvement of English usage.

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Authors and Affiliations

  1. Department of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok, 65000, Thailand

    Nareeluk Nakaew

  2. Centre of Excellent in Fungal Research, Faculty of Medical Science, Naresuan University, Phitsanulok, 65000, Thailand

    Nareeluk Nakaew

  3. Department of Agricultural Management Technology, Faculty of Science and Technology, Phranakhon Rajabhat University, Bangkhen, Bangkok, 10220, Thailand

    Chakrapong Rangjaroen

  4. Instituto de Recursos Naturales y Agrobiología de Sevilla (IRNAS-CSIC), Apartado 1052, Seville, 41080, Spain

    Rungroch Sungthong

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  1. Nareeluk Nakaew
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Correspondence to Nareeluk Nakaew.

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Nakaew, N., Rangjaroen, C. & Sungthong, R. Utilization of rhizospheric Streptomyces for biological control of Rigidoporus sp. causing white root disease in rubber tree. Eur J Plant Pathol 142, 93–105 (2015). https://doi.org/10.1007/s10658-015-0592-0

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