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Reduced Stratifin Expression Can Serve As an Independent Prognostic Factor for Poor Survival in Patients with Esophageal Squamous Cell Carcinoma

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Abstract

Stratifin plays an important role in cancer biology by interfering with intracellular signalling pathways and cell-cycle checkpoints. Decreased expression of stratifin gene has been reported to be a poor prognostic indicator in a variety of human malignant tumors.

Aim

To clarify the role and prognostic significance of stratifin in esophageal squamous cell carcinoma (ESCC).

Methods

The alteration of stratifin messenger RNA (mRNA) and protein was analyzed by reverse-transcription and quantitative real-time polymerase chain reaction (QRT-PCR) and Western blotting in 20 paired ESCC and nonneoplastic esophageal mucosa tissues, respectively. Then, immunohistochemistry (IHC) was used to evaluate expression of stratifin in tissues of 148 ESCC patients (including the former 20 pairs of tissues) and correlate it with clinicopathological parameters and prognosis of ESCC patients.

Results

The stratifin level of mRNA and protein was markedly downregulated in ESCC tissue compared with in corresponding nonneoplastic esophageal epithelium (P < 0.05). Similarly, the positive rate of stratifin protein expression was lower in the esophageal cancer than in paired nonneoplastic esophageal epithelium as detected by IHC (P = 0.007). Statistically, the downregulation of stratifin expression was correlated with tumor infiltration depth (P = 0.003), lymph node metastasis (P = 0.008), distant metastasis (P = 0.013), and lymphovascular invasion (P = 0.007) of ESCC. Furthermore, the reduced stratifin expression was associated with shorter 5-year survival rate of ESCC patients after curative surgery (P < 0.0001). On the basis of univariate and multivariate Cox regression analysis, we found that reduced stratifin expression, T4 stage, lymph node metastasis, and distant metastasis were independent risk factors for worse prognosis in ESCC patients.

Conclusion

The present report indicates that stratifin could be a useful indicator for prognosis of this disease, as well as a potential target for more effective therapy.

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Acknowledgments

This work was supported partly by a grant from the Innovation Project of Central South University (no. 2340-76208). We would like to thank Dr. Yong-Zeng for his assistance in English proofreading.

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Authors and Affiliations

  1. Department of Pathology, Xiangya Basic Medical College, Central South University, Changsha, China

    Hong-Zheng Ren, Guo-qing Pan, Ji-Fang Wen, Kuan-Song Wang & Geng-Qiu Luo

  2. Department of Pathology, Changzhi Medical College, Changzhi, China

    Jin-Sheng Wang

  3. Department of Health Statistics and Epidemiology, Public Health College, Central South University, Changsha, China

    Xu-zheng Shan

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  1. Hong-Zheng Ren
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  2. Guo-qing Pan
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  3. Jin-Sheng Wang
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Correspondence to Ji-Fang Wen.

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Hong-Zheng Ren and Guo-qing Pan contributed equally to this work.

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Ren, HZ., Pan, Gq., Wang, JS. et al. Reduced Stratifin Expression Can Serve As an Independent Prognostic Factor for Poor Survival in Patients with Esophageal Squamous Cell Carcinoma. Dig Dis Sci 55, 2552–2560 (2010). https://doi.org/10.1007/s10620-009-1065-0

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