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Differentiation of the Roles of Sulfide Oxidase and Rhodanese in the Detoxification of Sulfide by the Colonic Mucosa

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Abstract

Purpose: Identify the roles of sulfide oxidase and rhodanese in sulfide detoxification in rat colonic mucosa. Results: Gel filtration of colonic mucosa and purified bovine rhodanese showed that rhodanese and sulfide oxidizing activities resided in different proteins. In the presence of cyanide, rhodanese shifted the major mucosal metabolite of sulfide from thiosulfate to thiocyanate. The purported ability of purified rhodanese to metabolize sulfide reflects: (a) contamination with a sulfide oxidase, and (b) the spontaneous conversion of sulfide to thiosulfate during storage; rhodanese then catalyzes the conversion of this thiosulfate to thiocyanate. Conclusions: Rhodanese does not metabolize sulfide. The rate-limiting step in sulfide detoxification is oxidation by a sulfide oxidase to thiosulfate. Rhodanese then converts this thiosulfate to thiocyanate, but this reaction does not increase the rate of sulfide detoxification. The recent use of rhodanese activity as a surrogate for the rate that colonic mucosa detoxifies sulfide is inappropriate.

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References

  1. Ellenhorn MJ (1997) Respiratory toxicology. In: Ellenhorn MJ (ed) Ellenhorn’s medical toxicology: diagnosis and treatment of human poisoning. Williams and Wilkins, Maryland pp 1489–1493

    Google Scholar 

  2. Roediger WEW, Duncan A, Kapaniris O, Millard S (1993) Sulphide impairment of substrate oxidation in rat colonocytes: a biochemical basis for ulcerative colitis? Clin Sci 85:623–627

    PubMed  CAS  Google Scholar 

  3. Levitt MD, Furne J, Springfield J, Suarez F, DeMaster E (1999) Detoxification of hydrogen sulfide and methanethiol in the cecal mucosa. J Clin Invest 104:1107–1114

    Article  PubMed  CAS  Google Scholar 

  4. Picton R, Eggo MC, Merrill GA, Langman MJ, Singh S (2002) Mucosal protection against sulphide: importance of the enzyme rhodanese. Gut 50:201–205

    Article  PubMed  CAS  Google Scholar 

  5. Attene-Ramos MS, Wagner ED, Plew MJ, Gaskins HR (2006) Evidence that hydrogen sulfide is a genotoxic agent. Mol Cancer Res 4:9–14

    Article  PubMed  CAS  Google Scholar 

  6. Billaut-Laden I, Allorge D, Crunelle-Thibaut A, Rat E, Cauffiez C, Chevalier D, Houdret N, Lo-Guidice JM, Broly F (2006) Evidence for a functional genetic polymorphism of the human thiosulfate sulfurtransferase (Rhodanese), a cyanide and H2S detoxification enzyme. Toxicology 225:1–11

    PubMed  CAS  Google Scholar 

  7. Boehning D, Snyder SH (2003) Novel neural modulators. Ann Rev Neurosci 26:105–131

    Article  PubMed  CAS  Google Scholar 

  8. Huycke MM, Gaskins HR (2004) Commensal bacteria, redox stress, and colorectal cancer: mechanisms and models. Exp Biol Med 7:586–597

    Google Scholar 

  9. Leschelle X, Goubern M, Andriamihaja M, Blottiere HM, Couplan E, Gonzalez-Barroso MD, Petit C, Paqniez A, Chaumontet C, Mignotte B, Bouillaud F, Blachier F (2005) Adaptative metabolic response of human colonic epithelial cells to the adverse effects of the luminal compound sulfide. Biochim Biophys Acta 1725:201–212

    PubMed  CAS  Google Scholar 

  10. Mann NS, Rossaro L (2006) Sudden infant death syndrome: the colon connection. Med Hypotheses 66:375–379

    Article  PubMed  CAS  Google Scholar 

  11. Nozaki T, Ali V, Tokoro M (2005) Sulfur-containing amino acid metabolism in parasitic protozoa. Adv Parasitol 60:1–99

    Article  PubMed  Google Scholar 

  12. Schmedes A, Nielsen JN, Hey H, Brandslund I (2004) Low S-adenosylmethionine concentrations found in patients with severe inflammatory bowel disease. Clin Chem Lab Med 42:648–653

    Article  PubMed  CAS  Google Scholar 

  13. Teague B, Asiedu I, Moore PK (2002) The smooth muscle relaxant effect of hydrogen sulphide in vitro: evidence for a physiological role to control intestinal contractility. Br J Pharmacol 137:139–145

    Article  PubMed  CAS  Google Scholar 

  14. Williams RAM, Kelly SM, Mottram JC, Coombs GH (2003) 3-mercaptopyruvate sulfurtransferase of Leishmania contains an unusual C-terminal extension and is involved in thioredoxin and antioxidant metabolism. J Biol Chem 278:1480–1486

    Article  PubMed  CAS  Google Scholar 

  15. Ramasamy S, Singh S, Taniere P, Langman MJ, Eggo MC (2006) Sulfide-detoxifying enzymes in the human colon are decreased in cancer and upregulated in differentiation. Am J Physiol Gastrointest Liver Physiol 291:G288–G296

    Article  PubMed  CAS  Google Scholar 

  16. Picton R, Eggo MC, Langman MJS, Singh S (2007) Impaired detoxification of hydrogen sulfide in ulcerative colits? Dig Dis Sci 52:373–378

    Article  PubMed  CAS  Google Scholar 

  17. Westley J (1973) Rhodanese. Adv Enzymol 39:327–368

    PubMed  CAS  Google Scholar 

  18. Furne JK, Springfield J, Koenig T, DeMaster E, Levitt MD (2001) Oxidation of hydrogen sulfide and methanethiol to thiosulfate by rat tissues: a specialized function of the colonic mucosa. Biochem Pharmacol 62:255–259

    Article  PubMed  CAS  Google Scholar 

  19. Levitt MD, Springfield J, Furne J, Koenig T, Suarez SL (2001) Physiology of sulfide in the rat colon: use of bismuth to assess colonic sulfide production. J Appl Physiol 92:1660–1665

    Google Scholar 

  20. Sorbo BH (1955) Rhodanese. In: Colowick S, Kaplan NO (eds) Methods in enzymol. New York, Academic Press, pp 334–337

    Chapter  Google Scholar 

  21. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685

    Article  PubMed  CAS  Google Scholar 

  22. Windholz M, Budvari S, Blumetti RF, Otterbein ES (1983) The Merck manual, 10th edn. Merck and Co, New Jersey pp 1242

    Google Scholar 

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Authors and Affiliations

  1. Research Service (151) Mpls, Veterans Affairs Medical Center (VAMC), 1 Veterans Drive, Minneapolis, MN, 55417, USA

    Kirk Wilson, Mitchell Mudra, Julie Furne & Michael Levitt

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  1. Kirk Wilson
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  2. Mitchell Mudra
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  3. Julie Furne
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  4. Michael Levitt
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Correspondence to Michael Levitt.

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This work was supported by funds from the Department of Veterans Affairs and the Minnesota Veterans Research Institute and the Eli and Edythe L. Broad Foundation.

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Wilson, K., Mudra, M., Furne, J. et al. Differentiation of the Roles of Sulfide Oxidase and Rhodanese in the Detoxification of Sulfide by the Colonic Mucosa. Dig Dis Sci 53, 277–283 (2008). https://doi.org/10.1007/s10620-007-9854-9

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