Abstract
This study aims to investigate the function and mechanism of microRNA-106b-5p (miR-106b-5p) in cervical cancer (CC). Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was performed to determine miR-106b-5p expression in CC tissues and normal gastric tissues. Cell counting kit-8 (CCK-8) and colony formation assays were used to analyze the regulatory effects of miR-106b-5p on CC cells’ proliferative ability. Wound healing and Transwell assays were conducted to detect the effects of miR-106b-5p on cell migration and invasion. Besides, TargetScan was used to predict the potential target genes of miR-106b-5p. The interaction between miR-106b-5p and fibroblast growth factor 4 (FGF4) was proved by qRT-PCR, Western blot, and dual-luciferase reporter gene assay. MiR-106b-5p expression was down-regulated in CC tissues compared to non-tumorous tissues. The expression of miR-106b-5p was associated with the lymphatic node metastasis, FIGO stage and differentiation of CC. Functional assays revealed that miR-106b-5p overexpression suppressed CC cell proliferation, migration and invasion while miR-106b-5p inhibitor had the opposite effects. In addition, FGF4 was identified as a target gene of miR-106b-5p, and FGF could be negatively regulated by miR-106b-5p. MiR-106b-5p may serve as a tumor suppressor in CC, which can inhibit CC growth and metastasis by down-regulating FGF4 expression.
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Data availability
The data used to support the findings of this study are available from the corresponding author upon request.
Abbreviations
- 3′UTRs:
-
3′ Untranslated regions
- CC:
-
Cervical cancer
- CCK-8:
-
Cell counting kit-8
- FGF4:
-
Fibroblast growth factor 4
- FGFs:
-
Fibroblast growth factors
- MiR-106b-5p:
-
MicroRNA-106b-5p
- miRs:
-
MicroRNAs
- qRT-PCR:
-
Quantitative reverse transcription-polymerase chain reaction
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Acknowledgements
We thank Hubei Yican Health Industry Co., Ltd. (Wuhan, China) for its linguistic assistance during the preparation of this manuscript.
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This study is supported by the Natural Science Foundation of Dalian Medical University.
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Conceived and designed the experiments: XXY and FZJ; Performed the experiments: LHW, LJ and XXY; Analyzed the data: LHW and LJ; Wrote the paper: LHW and LJ.
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Hongwei, L., Juan, L., Xiaoying, X. et al. MicroRNA-106b-5p (miR-106b-5p) suppresses the proliferation and metastasis of cervical cancer cells via down-regulating fibroblast growth factor 4 (FGF4) expression. Cytotechnology 74, 469–478 (2022). https://doi.org/10.1007/s10616-022-00536-0
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DOI: https://doi.org/10.1007/s10616-022-00536-0