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Enhanced bacterial cellulose production from Gluconobacter xylinus using super optimal broth

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Abstract

The bacterial cellulose (BC) produced by Gluconobacter xylinus due to its versatile properties, is used in healthcare and industrial applications. However, its use is restricted owing to the limited yield from the existing culture protocols. In the current study, BC production is studied in the presence of Super Optimal Broth with catabolite repression (SOC) medium which is used to revive Escherichia coli cells after electroporation or chemoporation. In SOC medium, Gluconobacter xylinus produces cellulose pellicles within 5 days of incubation with an enhanced conversion of the carbon source to cellulose compared to traditional Hestrin–Schramm (HS) medium. SOC medium also maintains the pH close to 7.0 in static cultures unlike in HS medium where the pH is acidic. The physico-chemical and morphological characteristics of the BC produced in SOC are determined using powder X-ray diffraction (pXRD), thermo gravimetric analysis (TGA), Brunauer–Emmett–Teller (BET) and Barrett–Joyner–Halenda (BJH), and scanning electron microscopy (SEM) analyses. Our results indicate that SOC enhance the yield of bacterial cellulose and allows conversion of 50% of the carbon source to bacterial cellulose, compared to only 7% conversion in the case of traditional HS medium after 7 days of interaction. We also observe an increase in hydration capacity of BC produced using SOC as compared to HS media.

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Acknowledgments

The authors thank INST for financial support. PTC thanks INST for postdoctoral fellowship. Dr. Sharmistha acknowledges DST SERB for Women Excellence Award, 2017 (SB/WEA/06/2016) and INST for financial support. The authors thank Dr. Sangita Roy for helpful suggestions and discussions. The authors thank past and present members of Sinha lab for suggestions and discussions.

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  1. Institute of Nano Science and Technology (An Autonomous Institute Supported by Department of Science and Technology, Govt. of India), Habitat Center, Sector 64, SAS Nagar, Mohali, Punjab, India

    Prathna T. Chandrasekaran, Naimat Kalim Bari & Sharmistha Sinha

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  1. Prathna T. Chandrasekaran
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  2. Naimat Kalim Bari
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Correspondence to Sharmistha Sinha.

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Chandrasekaran, P.T., Bari, N.K. & Sinha, S. Enhanced bacterial cellulose production from Gluconobacter xylinus using super optimal broth. Cellulose 24, 4367–4381 (2017). https://doi.org/10.1007/s10570-017-1419-2

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