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Influence of thermodisinfection and duration of cryopreservation at different temperatures on pull out strength of cancellous bone

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Abstract

Thermodisinfection of human femoral heads from living donors harvested during hip joint replacement is an established processing procedure. This study was designed to examine the influence of heat sterilization on pull out strength of cancellous bone and storage at different temperatures up to 2 years since we had previously studied the storage of unprocessed cancellous bone. Porcine cancellous bone resembling human bone structure was obtained from 140 proximal humerus of 6–8 months old piglets. Pull out strength of screws after thermodisinfection was compared with unprocessed cancellous bone and tested immediately and after 6, 12 and 24 months of storage at −20 and −80 °C. A three-way ANOVA was performed and significance level was 5 %. The thermodisinfected bone showed a pull out force of 2729 N (1657–3568 N). The reduction of pull out strength compared with unprocessed bone over all periods of storage was 276 N on average with 95 % confidence interval ranging from 166 N to 389 N (p < 0.0001). Different freezing temperatures did not influence this mechanic property within 24 months storage and showed no difference compared with fresh frozen bone. Thermodisinfection of cancellous bone preserves tensile strength necessary for clinical purposes. The storage at −20 °C for at least 2 years did not show relevant decrease of pull out strength compared with −80 °C without difference between thermodisinfected and fresh frozen bone. The increase of the storage temperature to −20 °C for at least 2 years should be considered.

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Acknowledgments

We are particularly grateful for the support offered during the biomechanical examination to Prof. Dr. Ing. M. Niebert, Fachhochschule Gießen/Friedberg. Department Biomedizin und Technologie, Gießen and to Prof. Dr. H. Stürz, Director of the Orthopaedic University Clinic, Gießen.

Conflict of interest

No benefits in any form have been received or will be received from a commercial party related directly or indirectly to the subject of this article.

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Authors and Affiliations

  1. Department of Orthopaedic Surgery, Philipps University Medical School, Philipps University Marburg, Baldingerstrasse, 35043, Marburg, Germany

    Christian Fölsch

  2. Department of Orthopaedic Surgery, University Medical School, Doberaner Strasse, 18057, Rostock, Germany

    Wolfram Mittelmeier

  3. Department of Orthopaedic and Trauma Surgery, St. Willehad Hospital, Ansgaristrasse 12, 26382, Wilhelmshaven, Germany

    Thomas von Garrel

  4. Bahnhofstrasse 2, 35321, Laubach, Germany

    Uwe Bilderbeek

  5. Institute of Medical Biometry and Epidemiology, Philipps University Marburg, Bunsenstrasse 3, 35037, Marburg, Germany

    Nina Timmesfeld

  6. University Tissue Bank, Institute of Transfusion Medicine, Charité University Medical School, Charitéplatz 1, 10117, Berlin, Germany

    Axel Pruss

  7. GNG – Gesundheitsnetz Gießen, Frankfurter Strasse 1, 35390, Gießen, Germany

    Hans-Peter Matter

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  1. Christian Fölsch
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Correspondence to Christian Fölsch.

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Fölsch, C., Mittelmeier, W., von Garrel, T. et al. Influence of thermodisinfection and duration of cryopreservation at different temperatures on pull out strength of cancellous bone. Cell Tissue Bank 16, 73–81 (2015). https://doi.org/10.1007/s10561-014-9442-0

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