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Zinc transporter expression profiles in the rat prostate following alterations in dietary zinc

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Abstract

Zinc plays important roles in numerous cellular activities and physiological functions. Intracellular zinc levels are strictly maintained by zinc homeostatic mechanisms. Zinc concentrations in the prostate are the highest of all soft tissues and could be important for prostate health. However, the mechanisms by which the prostate maintains high zinc levels are still unclear. In addition, the response of the prostate to alterations in dietary zinc is unknown. The current study explored cellular zinc levels and zinc transporter expression profiles in the lobes of the prostate during dietary marginal zinc depletion. Rats were given either zinc-adequate (ZA, 30 mg Zn/kg) or marginal zinc-deficient (MZD, 5 mg Zn/kg) diet for 9 weeks. In addition, a subgroup of the MZD rats was supplemented with phytase (1,500 unit/kg diet) to improve zinc bioavailability. We found that both zinc concentrations and ZnT2 expression in the prostate dorsolateral lobes were substantially higher than in the ventral lobes (P < 0.05). Marginal zinc depletion significantly decreased ZnT2 expression in the dorsolateral lobes (P < 0.05), and phytase supplementation had a trend to increase ZnT2 expression. In addition, of all measured zinc transporters, only ZnT2 mRNA abundance was significantly correlated to the zinc concentrations in the dorsolateral lobe. No correlations were found between zinc transporter expression and zinc concentrations in the ventral lobes. These results indicate that ZnT2 may play a significant role in the maintenance of zinc homeostasis in the prostate.

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Acknowledgments

We thank Dr. Shannon Kelleher for the generous gift of ZnT2 antibodies. We gratefully acknowledge the WM Keck Collaboratory at Oregon State University for their assistance in conducting these studies. This study was funded by Oregon AES (OR00735), and the Environmental Health Science Center at Oregon State University (NIEHS P30 ES00210) and by the US Army MRMC. The opinions or assertions contained herein are the private views of the authors and are not to be construed as official or as reflecting the views of the Army or the Department of Defense.

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Authors and Affiliations

  1. Department of Nutrition and Exercise Sciences, Oregon State University, 103 Milam Hall, Corvallis, OR, 97331, USA

    Yang Song, Valerie Elias, Carmen P. Wong & Emily Ho

  2. Military Nutrition Division, US Army Research Institute of Environmental Medicine, Kansas St., Natick, MA, 01760, USA

    Angus G. Scrimgeour

  3. Linus Pauling Institute, Oregon State University, Corvallis, OR, 97331, USA

    Emily Ho

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  1. Yang Song
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  2. Valerie Elias
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  3. Carmen P. Wong
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  4. Angus G. Scrimgeour
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  5. Emily Ho
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Correspondence to Emily Ho.

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Song, Y., Elias, V., Wong, C.P. et al. Zinc transporter expression profiles in the rat prostate following alterations in dietary zinc. Biometals 23, 51–58 (2010). https://doi.org/10.1007/s10534-009-9266-8

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  • DOI: https://doi.org/10.1007/s10534-009-9266-8

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