Abstract
Headwater streams are foci for nutrient and energy loading from terrestrial landscapes, in situ nutrient transformations, and downstream transport. Despite the prominent role that headwater streams can have in regulating downstream water quality, the relative importance of processes that can influence nutrient uptake have not been fully compared in heterotrophic aquatic systems. To address this research need, we assessed the seasonality of dissolved organic carbon (DOC) and nitrate (NO3−) uptake, compared the relative influence of hydrologic and biogeochemical drivers on observed seasonal trends in nutrient uptake, and estimated the influence of these biological transformations on watershed scale nutrient retention and export. We determined that seasonal reductions in DOC and NO3− concentrations led to decreases in the potential for the biotic community to take up nutrients, and that seasonality of DOC and NO3− uptake was consistent with the seasonal dynamics of ecosystem metabolism. We calculated that that during the post-snowmelt period (June to August), biotic retention of both dissolved organic carbon and nitrate exceeded export fluxes from this headwater catchment, highlighting the potential for biological processes to regulate downstream water quality.
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References
Ågren AM, Buffam I, Cooper DM et al (2014) Can the heterogeneity in stream dissolved organic carbon be explained by contributing landscape elements? Biogeosciences 11:1199–1213. https://doi.org/10.5194/bg-11-1199-2014
Aguilera R, Marcé R, Sabater S (2013) Modeling nutrient retention at the watershed scale: does small stream research apply to the whole river network? J Geophys Res Biogeosci 118(2):728–740
Alexander RB, Smith RA, Schwarz GE (2000) Effect of stream channel size on the delivery of nitrogen to the Gulf of Mexico. Nature 403:758–762. https://doi.org/10.1038/35001562
Basu NB, Destouni G, Jawitz JW et al (2010) Nutrient loads exported from managed catchments reveal emergent biogeochemical stationarity. Geophys Res Lett 37:1–5. https://doi.org/10.1029/2010GL045168
Battin TJ, Kaplan LA, Findlay S et al (2008) Biophysical controls on organic carbon fluxes in fluvial networks. Nat Geosci 2:595. https://doi.org/10.1038/ngeo602
Bennett JP, Rathburn RE (1972) Reaeration in open-channel flow. Geological Survey Professional Paper 737, U. S. Government Printing Office, Washington, DC
Berggren M, Giorgio PA (2015) Distinct patterns of microbial metabolism associated with riverine dissolved organic carbon of different source and quality. J Geophys Res Biogeosci. https://doi.org/10.1002/2015JG002963
Berggren M, Laudon H, Jansson M (2009) Hydrological control of organic carbon support for bacterial growth in boreal headwater streams. Microb Ecol 57:170–178. https://doi.org/10.1007/s00248-008-9423-6
Berggren M, Laudon H, Haei M, Stro L (2010) Efficient aquatic bacterial metabolism of dissolved low-molecular-weight compounds from terrestrial sources. ISME J. https://doi.org/10.1038/ismej.2009.120
Bergstrom A, McGlynn B, Mallard J, Covino T (2016) Watershed structural influences on the distributions of stream network water and solute travel times under baseflow conditions. Hydrol Process 30:2671–2685. https://doi.org/10.1002/hyp.10792
Bernal S, Von Schiller D, Martí E, Sabater F (2012) In-stream net uptake regulates inorganic nitrogen export from catchments under base flow conditions. J Geophys Res Biogeosci 117:1–10. https://doi.org/10.1029/2012JG001985
Bernal S, Lupon A, Ribot M et al (2015) Riparian and in-stream controls on nutrient concentrations and fluxes in a headwater forested stream. Biogeosciences 12:1941–1954. https://doi.org/10.5194/bg-12-1941-2015
Bernhardt ES, Likens GE (2002) Dissolved organic carbon enrichment alters stream nitrogen dynamics in a forest stream. Ecology 83:1689–1700. https://doi.org/10.1890/0012-9658(2002)083[1689:DOCEAN]2.0.CO;2
Bernhardt ES, Hall RO Jr, Likens GE (2002) Whole-system estimates of nitrification and nitrate uptake in streams of the hubbard brook experimental forest. Ecosystems 5:419–430. https://doi.org/10.1007/s10021-002-0179-4
Bernhardt ES, Likens GE, Hall RO et al (2005) Can’t see the forest for the stream? In-stream processing and terrestrial nitrogen exports. Bioscience 55:219–230. https://doi.org/10.1641/0006-3568(2005)055[0219:ACSTFF]2.0.CO;2
Brookshire ENJ, Valett HM, Thomas SA et al (2005) Coupled cycling of dissolved organic nitrogen and carbon in a forest stream. Ecology 86:2487–2496. https://doi.org/10.1890/04-1184
Brookshire ENJ, Valett HM, Gerber S (2009) Maintenance of terrestrial nutrient loss signatures during in-stream transport. Ecology 90:293–299. https://doi.org/10.1890/08-0949.1
Cohen MAJ, Kurz MJ, Heffernan JB et al (2013) Diel phosphorus variation and the stoichiometry of ecosystem metabolism in a large spring-fed river. Ecol Monogr 83:155–176
Cole JJ, Prairie YT, Caraco NF et al (2007) Plumbing the global carbon cycle: Integrating inland waters into the terrestrial carbon budget. Ecosystems 10:172–185. https://doi.org/10.1007/s10021-006-9013-8
Cory RM, Kaplan LA (2012) Biological lability of streamwater fluorescent dissolved organic matter. Limnol Oceanogr 57:1347–1360. https://doi.org/10.4319/lo.2012.57.5.1347
Cory RM, Ward CP, Crump BC, Kling GW (2014) Sunlight controls water column processing of carbon in arctic fresh waters. Science 345(80):925–928. https://doi.org/10.1126/science.1253119
Cory RM, Harrold KH, Neilson BT, Kling GW (2015) Controls on dissolved organic matter (DOM) degradation in a headwater stream: the influence of photochemical and hydrological conditions in determining light-limitation or substrate-limitation of photo-degradation. Biogeosci Discuss 12:9793–9838. https://doi.org/10.5194/bgd-12-9793-2015
Covino T, McGlynn BL (2007) Stream gains and losses across a mountain-to-valley transition: Impacts on watershed hydrology and stream water chemistry. Water Resour Res 43:1–14. https://doi.org/10.1029/2006WR005544
Covino T, McGlynn BL, Baker M (2010a) Separating physical and biological nutrient retention and quantifying uptake kinetics from ambient to saturation in successive mountain stream reaches. J Geophys Res Biogeosci 115:1–17. https://doi.org/10.1029/2009JG001263
Covino T, McGlynn BL, McNamara RA (2010b) Tracer additions for spiraling curve characterization (TASCC): quantifying stream nutrient uptake kinetics from ambient to saturation. Limnol Oceanogr Methods 8:484–498. https://doi.org/10.4319/lom.2010.8.484
Covino T, McGlynn BL, Mallard J (2011) Stream-groundwater exchange and hydrologic turnover at the network scale. Water Resour Res 47:1–11. https://doi.org/10.1029/2011WR010942
Covino T, McGlynn BL, McNamara R (2012) Land use/land cover and scale influences on in-stream nitrogen uptake kinetics. J Geophys Res 117:1–13. https://doi.org/10.1029/2011JG001874
Crawford JT, Lottig NR, Stanley EH et al (2014) CO2 and CH4 emissions from streams in a lake-rich landscape: patterns, controls, and regional significance. Global Biogeochem Cycles. https://doi.org/10.1002/2013GB004661
Creed IF, McKnight DM, Pellerin BA et al (2015) The river as a chemostat: fresh perspectives on dissolved organic matter flowing down the river continuum. Can J Fish Aquat Sci 72:1–37
del Giorgio PA, Cole JJ (1998) Bacterial growth efficiency in natural aquatic systems. Annu Rev Ecol Syst 29:503–541
Demars BOL, Thompson J, Manson JR (2015) Stream metabolism and the open diel oxygen method: Principles, practice, and perspectives. Limnol Oceanogr Methods 13:356–374. https://doi.org/10.1002/lom3.10030
Duarte CM, Prairie YT (2005) Prevalence of heterotrophy and atmospheric CO2 emissions from aquatic ecosystems. Ecosystems 8:862–870. https://doi.org/10.1007/s10021-005-0177-4
Elser JJ, Bracken MES, Cleland EE et al (2007) Global analysis of nitrogen and phosphorus limitation of primary producers in freshwater, marine and terrestrial ecosystems. Ecol Lett 10:1135–1142. https://doi.org/10.1111/j.1461-0248.2007.01113.x
Ensign SH, Doyle MW (2005) In-channel transient storage and associated nutrient retention: evidence from experimental manipulations. Limnol Oceanogr 50:1740–1751. https://doi.org/10.4319/lo.2005.50.6.1740
Ensign SH, Doyle MW (2006) Nutrient spiraling in streams and river networks. J Geophys Res Biogeosci 111:1–13. https://doi.org/10.1029/2005JG000114
Fellows CS, Valett HM, Dahm CN et al (2006) Coupling nutrient uptake and energy flow in headwater streams. Ecosystems 9:788–804. https://doi.org/10.1007/s10021-006-0005-5
Findlay S, Sobczak WV (1996) Variability in removal of dissolved organic carbon in hyporheic sediments. J North Am Benthol Soc 15:35–41
Fisher SG, Likens GE (1973) Energy flow in bear Brook, New Hampshire: an integrative approach to stream ecosystem metabolism. Ecol Monogr 43:421–439
Gardner KK, McGlynn BL, Marshall LA (2011) Quantifying watershed sensitivity to spatially variable N loading and the relative importance of watershed N retention mechanisms. Water Resour Res 47:1–21. https://doi.org/10.1029/2010WR009738
Genereux DP, Hemond HH (1992) Determination of gas exchange rate constants for a small stream on walker branch watershed, Tennessee. Water Resour Res 28:2365–2374. https://doi.org/10.1029/92WR01083
Godsey SE, Kirchner JW, Clow DW (2009) Concentration—discharge relationships reflect chemostatic characteristics of US catchments. Hydrol Process 23:1844–1864. https://doi.org/10.1002/hyp.7315
Gomez-Velez JD, Harvey JW, Cardenas MB, Kiel B (2015) Denitrification in the Mississippi River network controlled by flow through river bedforms. Nat Geosci. https://doi.org/10.1038/ngeo2567
Grimm NB (1987) Nitrogen dynamics during succession in a desert stream. Ecology 68:1157–1170. https://doi.org/10.2307/1939200
Grimm NB, Fisher SG (1989) Stability of periphyton and macroinvertebrates to disturbance by flash floods in a desert stream. J North Am Benthol Soc 8:293–307
Hall RO, Tank JL (2003) Ecosystem metabolism controls nitrogen uptake in streams in Grand Teton National Park, Wyoming. Limnol Oceanogr 48:1120–1128. https://doi.org/10.4319/lo.2003.48.3.1120
Hall RO, Bernhardt ES, Likens GE (2002) Relating nutrient uptake with transient storage in forested mountain streams. Limnol Oceanogr 47:255–265. https://doi.org/10.4319/lo.2002.47.1.0255
Hall RO, Baker MA, Arp CD, Koch BJ (2009a) Hydrologic control of nitrogen removal, storage, and export in a mountain stream. Limnol Oceanogr 54:2128–2142. https://doi.org/10.4319/lo.2009.54.6.2128
Hall RO, Tank JL, Sobota DJ et al (2009b) Nitrate removal in stream ecosystems measured by Total uptake 15 N addition experiments: total uptake. Limnol Oceanogr 54:653–665
Hanley KW, Wollheim WM, Salisbury J et al (2013) Controls on dissolved organic carbon quantity and chemical character in temperate rivers of North America. Global Biogeochem Cycles 27:492–504. https://doi.org/10.1002/gbc.20044
Harpole WS, Ngai JT, Cleland EE et al (2011) Nutrient co-limitation of primary producer communities. Ecol Lett 14:852–862. https://doi.org/10.1111/j.1461-0248.2011.01651.x
Harvey J (2016) Hydrologic exchange flows and their ecological consequences in river corridors. In: Stream ecosystems in a changing environment, pp 1–83. https://doi.org/10.1016/B978-0-12-405890-3.00001-4
Harvey JW, Wagner BJ, Bencala KE (1996) Evaluating the reliability of the stream tracer approach to characterize stream-subsurface water exchange. Water Resour Res 32:2441–2451. https://doi.org/10.1029/96WR01268
Heffernan JB, Cohen MJ (2010) Direct and indirect coupling of primary production and diel nitrate dynamics in a subtropical spring-fed river. Limnol Oceanogr 55:677–688. https://doi.org/10.4319/lo.2009.55.2.0677
Hoellein TJ, Tank JL, Rosi-Marshall EJ et al (2007) Controls on spatial and temporal variation of nutrient uptake in three Michigan headwater streams. Limnol Oceanogr 52:1964–1977. https://doi.org/10.4319/lo.2007.52.5.1964
Hoellein TJ, Bruesewitz DA, Richardson DC (2013) Revisiting Odum (1956): a synthesis of aquatic ecosystem metabolism. Limnol Oceanogr 58:2089–2100. https://doi.org/10.4319/lo.2013.58.6.2089
Jencso KG, McGlynn BL, Gooseff MN et al (2010) Hillslope hydrologic connectivity controls riparian groundwater turnover: implications of catchment structure for riparian buffering and stream water sources. Water Resour Res. https://doi.org/10.1029/2009wr008818
Johnson MS, Lehmann J, Riha SJ et al (2008) CO2 efflux from Amazonian headwater streams represents a significant fate for deep soil respiration. Geophys Res Lett 35:L17401. https://doi.org/10.1029/2008GL034619
Johnson LT, Tank JL, Arango CP (2009) The effect of land use on dissolved organic carbon and nitrogen uptake in streams. Freshw Biol 54:2335–2350. https://doi.org/10.1111/j.1365-2427.2009.02261.x
Jones JB, Stanley EH, Mulholland PJ (2003) Long-term decline in carbon dioxide supersaturation in rivers across the contiguous United States. Geophys Res Lett 30:1495. https://doi.org/10.1029/2003GL017056
Kilpatrick FA, Cobb ED (1985) Measurement of discharge using tracers. Report of the U.S. Geological Survey, Techniques of Water Resources Investigations Book 3, Chap A16, pp 6–15
Kothawala DN, Ji X, Laudon H et al (2015) The relative influence of land cover, hydrology and in-stream processing on the composition of dissolved organic matter in boreal streams. J Geophys Res Biogeosciences 120:1–15. https://doi.org/10.1002/2015JG002946
Lambert T, Teodoru CR, Nyoni FC et al (2016) Degradation of dissolved organic matter in a large tropical river. Biogeosci Discuss 13:2727–2741. https://doi.org/10.5194/bg-2016-9
Lapierre J-F, Guillemette F, Berggren M, del Giorgio PA (2013) Increases in terrestrially derived carbon stimulate organic carbon processing and CO2 emissions in boreal aquatic ecosystems. Nat Commun 4:2972. https://doi.org/10.1038/ncomms3972
Lautz LK, Siegel DI (2007) The effect of transient storage on nitrate uptake lengths in streams: an inter-site comparison. Hydrol Process 21:3533–3548. https://doi.org/10.1002/hyp.6569
Likens GE, Bormann FH, Johnson NM et al (1970) Effects of forest cutting and herbicide treatment on nutrient budgets in the hubbard brook watershed-ecosystem. Ecol Monogr 40:23–47. https://doi.org/10.2307/1942440
Lupon A, Martí E, Sabater F, Bernal S (2015) Green light: gross primary production influences seasonal stream N export by controlling fine-scale N dynamics. Ecology 97:133–144. https://doi.org/10.1890/14-2296.1
Lutz BD, Bernhardt ES, Roberts BJ, Mulholland PJ (2011) Examining the coupling of carbon and nitrogen cycles in Appalachian streams: the role of dissolved organic nitrogen. Ecology 92:720–732. https://doi.org/10.1890/10-0899.1
Mallard J, McGlynn B, Covino T (2014) Lateral inflows, stream-groundwater exchange, and network geometry influence stream water composition. Water Resour Res 50:4603–4623. https://doi.org/10.1002/2013WR014944
Marti E, Sabater F (1996) High variability in temporal and spatial nutrient retention in mediterranean streams. Ecology 77:854–869. https://doi.org/10.2307/2265506
Marzolf ER, Mulholland PJ, Steinman AD (1994) Improvements to the diurnal upstream-downstream dissolved oxygen change technique for determining whole-stream metabolism in small streams. Can J Fish Aquat Sci 51:1591–1599. https://doi.org/10.1139/f94-158
Mason SJK, McGlynn BL, Poole GC (2012) Hydrologic response to channel reconfiguration on Silver Bow Creek, Montana. J Hydrol 438–439:125–136. https://doi.org/10.1016/j.jhydrol.2012.03.010
Melching CS, Flores HE, Flores HE (1999) Reaeration equations derived from U.S. Geological Survey database. J Environ Eng. https://doi.org/10.1061/(asce)0733-9372(1999)125:5(407)
Meyer JL, Likens GE (1979) Transport and transformation of phosphorus in a forest stream ecosystem. Ecology 60:1255. https://doi.org/10.2307/1936971
Mincemoyer SA, Birdsall JL (2006) Vascular flora of the tenderfoot creek experimental forest, Little Belt Mountains, Montana. Madrono 53:211–222. https://doi.org/10.3120/0024-9637(2006)53
Mineau MM, Wollheim WM, Buffam I et al (2016) Dissolved organic carbon uptake in streams: a review and assessment of reach-scale measurements. J Geophys Res Biogeosci. https://doi.org/10.1002/2015JG003204
Mulholland PJ (1992) Regulation of nutrient concentrations in a temperate forest stream: roles of upland, riparian, and instream processes. Limnol Oceanogr 37:1512–1526. https://doi.org/10.4319/lo.1992.37.7.1512
Mulholland PJ (2004) The importance of in-stream uptake for regulating stream concentrations and outputs of N and P from a forested watershed: evidence from long-term chemistry records for Walker Branch Watershed. Biogeochemistry 70:403–426. https://doi.org/10.1007/s10533-004-0364-y
Mulholland PJ, Marzolf ER, Webster JR, Hart DR (1997) Evidence that hyporheic zones increase heterotrophic metabolism and phosphorus uptake in forest streams. Limnol Oceanogr 42:443–451. https://doi.org/10.4319/lo.1997.42.3.0443
Mulholland PJ, Tank JL, Webster JR et al (2002) Can uptake length in streams be determined by nutrient addition experiments? Results from an interbiome comparison study. J North Am Benthol Soc 21:544–560. https://doi.org/10.2307/1468429
Mulholland PJ, Roberts BJ, Hill WR, Smith JG (2009) Stream ecosystem responses to the 2007 spring freeze in the southeastern United States: unexpected effects of climate change. Glob Chang Biol 15:1767–1776. https://doi.org/10.1111/j.1365-2486.2009.01864.x
Newbold JD, Elwood JW, O’Neill RV, van Winkle W (1981) Measuring nutrient spiralling in streams. Can J Fish Aquat Sci 38:860–863. https://doi.org/10.1139/f81-114
Pacific VJ, Jencso KG, McGlynn BL (2010) Variable flushing mechanisms and landscape structure control stream DOC export during snowmelt in a set of nested catchments. Biogeochemistry 99:193–211. https://doi.org/10.1007/s10533-009-9401-1
Patil S, Covino TP, Packman AI et al (2013) Intrastream variability in solute transport: hydrologic and geomorphic controls on solute retention. J Geophys Res Earth Surf 118:413–422. https://doi.org/10.1029/2012JF002455
Payn RA, Gooseff MN, McGlynn BL et al (2009) Channel water balance and exchange with subsurface flow along a mountain headwater stream in Montana, United States. Water Resour Res 45:1–14. https://doi.org/10.1029/2008WR007644
Pennino MJ, Kaushal SS, Beaulieu JJ et al (2014) Effects of urban stream burial on nitrogen uptake and ecosystem metabolism: implications for watershed nitrogen and carbon fluxes. Biogeochemistry 121:247–269. https://doi.org/10.1007/s10533-014-9958-1
Peterson BJ, Wollheim WM, Mulholland PJ et al (2001) Control of nitrogen export from watersheds by headwater streams. Science 292(80):86–90
Piper LR, Cross WF, McGlynn BL (2017) Colimitation and the coupling of N and P uptake kinetics in oligotrophic mountain streams. Biogeochemistry. https://doi.org/10.1007/s10533-017-0294-0
Raymond PA, Hartmann J, Lauerwald R et al (2013) Global carbon dioxide emissions from inland waters. Nature 503:355–359. https://doi.org/10.1038/nature12760
Raymond PA, Saiers JE, Sobczak WV (2016) Hydrological and biogeochemical controls on watershed dissolved organic matter transport: pulse-shunt concept. Ecology 97:5–16. https://doi.org/10.1890/07-1861.1
Redfield AC (1958) The biological control of chemical factors in the environment. Am Sci 46:205–221
Resh VH, Brown AV, Covich AP et al (1988) The role of disturbance in stream ecology. J North Am Benthol Soc 7:433–455. https://doi.org/10.2307/1467300
Roberts BJ, Mulholland PJ (2007) In-stream biotic control on nutrient biogeochemistry in a forested stream, West Fork of Walker Branch. J Geophys Res 112:G04002. https://doi.org/10.1029/2007JG000422
Schade JD, MacNeill K, Thomas SA et al (2011) The stoichiometry of nitrogen and phosphorus spiralling in heterotrophic and autotrophic streams. Freshw Biol 56:424–436. https://doi.org/10.1111/j.1365-2427.2010.02509.x
Schade JD, Seybold EC, Drake T et al (2016) Variation in summer nitrogen and phosphorus uptake among Siberian headwater streams. Polar Res 35:1–28. https://doi.org/10.3402/polar.v35.24571
Simon KS, Townsend CR, Biggs BJF, Bowden WB (2005) Temporal variation of N and P uptake in 2 New Zealand streams. J North Am Benthol Soc 24:1–18. https://doi.org/10.1899/0887-3593(2005)024<0001:TVONAP>2.0.CO;2
Sinsabaugh RL (1997) Large-scale trends for stream benthic respiration. J North Am Benthol Soc 16:119–122
Sinsabaugh RL, Turner BL, Talbot JM et al (2016) Stoichiometry of microbial carbin use efficiency in soils. Ecol Monogr 86:172–189. https://doi.org/10.1017/CBO9781107415324.004
Sterner RW, Elser JJ (2002) Ecological stoichiometry: the biology of elements from molecules to the biosphere. Princeton University Press, Princeton
Stream Solute Workshop (1990) Concepts and methods for assessing solute dynamics in stream ecosystems. J North Am Benthol Soc 9:95–119
Tank JJL, Rosi-Marshall EJE, Griffiths NA et al (2010) A review of allochthonous organic matter dynamics and metabolism in streams. J North Am Benthol Soc 29:118–146. https://doi.org/10.1899/08-170.1
Taylor PG, Townsend AR (2010) Stoichiometric control of organic carbon-nitrate relationships from soils to the sea. Nature 464:1178–1181. https://doi.org/10.1038/nature08985
Valett HM, Morrice JA, Dahm CN, Campana ME (1996) Parent lithology, surface-groundwater exchange, and nitrate retention in headwater streams. Limnol Oceanogr 41:333–345. https://doi.org/10.4319/lo.1996.41.2.0333
Vitousek PM, Aber JD, Howarth RW et al (1997) Human alteration of the global nitrogen cycle: sources and consequences. Ecol Appl 7:737–750
Webster JR, Mulholland PJ, Tank JL et al (2003) Factors affecting ammonium uptake in streams–an inter-biome perspective. Freshw Biol 48:1329–1352
Wilkinson GM, Pace ML, Cole JJ (2013) Terrestrial dominance of organic matter in north temperate lakes. Global Biogeochem Cycles 27:43–51. https://doi.org/10.1029/2012GB004453
Wollheim WM, Pellerin BA, Vörösmarty CJ, Hopkinson CS (2005) N retention in urbanizing headwater catchments. Ecosystems 8:871–884. https://doi.org/10.1007/s10021-005-0178-3
Wollheim WM, Vörösmarty CJ, Peterson BJ, Seitzinger SP, Hopkinson CS (2006) Relationship between river size and nutrient removal. Geophys Res Lett 33(6). https://doi.org/10.1029/2006GL025845.
Wollheim WM, Stewart RJ, Aiken GR et al (2015) Removal of terrestrial DOC in aquatic ecosystems of a temperate river network. Geophys Res Lett 42:6671–6679. https://doi.org/10.1002/2015GL064647
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Financial support for this project was provided by Duke University, the National Science Foundation (NSF) Graduate Research Fellowship Program, NSF Grant #1114392, and USDA Award #2012-67019-19360. We would like to thank Maggie Zimmer, Kendra Kaiser, Andrew Burch, and Patrick Clay for assistance with fieldwork. We thank the Tenderfoot Creek Experimental Forest for allowing us access to the site and providing logistical support.
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Seybold, E., McGlynn, B. Hydrologic and biogeochemical drivers of dissolved organic carbon and nitrate uptake in a headwater stream network. Biogeochemistry 138, 23–48 (2018). https://doi.org/10.1007/s10533-018-0426-1
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DOI: https://doi.org/10.1007/s10533-018-0426-1