Abstract
Carbon-based materials have been shown to enhance anaerobic digestion processes by promoting direct interspecies electron transfer in methanogenic consortia. However, little is known on their effects during the treatment of complex substrates, such as those derived from protein-rich wastewaters. Here, organic xerogels (OX) are tested, for the first time, as accelerators of the methanogenic activity of an anaerobic consortium treating a synthetic protein-rich wastewater. Three OX with distinct pore size distribution (10 and 1000 nm for OX-10 and OX-1000, respectively) and structural conformation (graphene oxide integration into OX-10-GO polymeric matrix) were synthesized. OX-1000 promoted the highest methane production rate (5.21 mL/g*h, 13.5% increase with respect to the control incubated without OX) among the synthesized OX. Additionally, batch bioreactors amended with OX achieved higher chemical oxygen demand (COD) removal (up to 88%) as compared to the control, which only showed 50% of COD removal. Interestingly, amendment of bioreactors with OX also triggered the production of medium-chain fatty acids, including caprylate and caproate. Moreover, OX decreased the accumulation of ammonium, derived from proteins hydrolysis, partly explained by their adsorption capacities, and probably involving their electron-accepting capacity promoting anaerobic ammonium oxidation. This is the first time that OX were successfully applied as methanogenic accelerators for the anaerobic treatment of synthetic protein-rich wastewater, increasing the methane production rate and COD removal as well as triggering the production of medium chain fatty acids and attenuating the accumulation of ammonium. Therefore, OX are proposed as suitable materials to boost the efficiency of anaerobic systems to treat complex industrial wastewaters.
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Acknowledgements
This work was financially supported by Universidad Nacional Autónoma de México (UNAM, PAPIIT Grant TA100120). LARM acknowledges fellow from UNAM (DGAPA grant number COIC/DDTPA/064/2020). The technical assistance of Gloria Moreno-Rodríguez and Jaime Pérez-Trevilla is greatly appreciated.
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Universidad Nacional Autónoma de México (UNAM, PAPIIT grant TA100120 and UNAM, DGAPA Grant Number COIC/DDTPA/064/2020).
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Conceptualization, FJC; visualization, LARM, FJC; formal analysis, LARM, FJC; investigation, LARM; methodology, MAMM, JRRM; validation, MAMM, JRRM; project administration, FJC; resources, FJC; writing—review and editing, LARM, FJC.
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Ramírez-Montoya, L.A., Montes-Morán, M.A., Rangel-Mendez, J.R. et al. Enhanced anaerobic treatment of synthetic protein-rich wastewater promoted by organic xerogels. Biodegradation 33, 255–265 (2022). https://doi.org/10.1007/s10532-022-09984-z
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DOI: https://doi.org/10.1007/s10532-022-09984-z