Abstract
Fungal influenced corrosion (FIC) of some corroded sites in three selected bridges [Embaba bridge (E-bridge), Kasr al-Nile-bridge (K-bridge) and University bridge (U-bridge)] located over the River Nile in Egypt were investigated. Six fungal species, belong to 12 fungal genera, were isolated from the corroded reinforced concrete of the three tested bridges. Fourier transform infrared spectroscopy (FTIR) was screened for the most dominant fungal species (Fusarium oxysporium) which showed in all tested bridges that indicated the presence of amine group accompanied with polysaccharides contents. FIC of the most deteriorated bridge (K-bridge) was documented with FTIR. The association of fungal spores with corrosion products was recorded with scanning electron microscope (SEM). Evaluation of ozone for preventing FIC of the K-bridge was carried out by recording the corrosion rate and the corresponding inhibition efficiency (IE%). No mycelial growth with 100% IE was observed at 3 ppm ozone concentration after 120 min exposure time. With longer duration of ozone exposure, the membrane permeability of F. oxysporium was compromised as indicated by protein and nucleic acid leakages accompanied with lipid and tryptophan oxidation. The total intracellular and extracellular proteins of F. oxysporium were run on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) indicated the increasing of the supernatant protein on the expense of the cellular protein bands with extending ozone exposure time (0–80 min).
Similar content being viewed by others
References
Ali EM (2006) Evaluation of ozone as inhibitory agent for microbial growth on contaminated dry herb and spices. MSc, Thesis, Faculty of Science, Biology Department, Cairo University
Ashwell G (1957) Methods in enzymology, vol. III. Interscience, New York, p 81
Atlas RM (1981) Microbial degradation of petroleum hydrocarbons: an environmental perspective. Microbiol rev 180–208
Aviam O, Nes G, Zeiri Y, Sivan A (2004) Accelerated biodegradation of cement by sulfur-oxidizing bacteria as a bioassay for evaluating immobilization of low-level radioactive waste. Appl Environ Microbiol 70:6031–6036
Azarpazhooh A (2008) The application of ozone in dentistry: a systematic review of literature. J Dent 36:104–116
Badcock NR, Zoanetti GD, Martin ES (1997) Nonchromatographic assay for malondialdehyde–thiobarbituric acid adduct with HPLC equivalence. Clin Chem 43:1655–1657
Barnett HL, Hunter BB (1972) Illustrated genera of imperfect fungi. Burgess Publishing Company, Minneapolis
Bocci V, Ozonoterapia O, Editrice C (2002) Oxygen-ozone therapy: a critical evaluation. Ambrosiana, Milan, Italy, 2000 (in Italian). Eng. Trans. Kluwer, Dordrecht
Bocci V, Borrelli E, Travagli V, Zanardi L (2009) The ozone paradox: ozone is a strong oxidant as well as a medical drug. Med Res Rev 29:646–682
Burton K (1968) Methods in enzymology, vol. II, Part B. Interscience, New York, p 163
Cataldo F (2006) Ozone degradation of biological macromolecules: proteins, hemoglobin, RNA, and DNA. Ozone Sci Eng 28:317–328
Chen Z, Gallie D (2004) Increasing tolerance to ozone by elevating foliar ascorbic acid confers greater protection against ozone than increasing avoidance. Plant Physiol 138:1673–1689
Choe D, Gardoni P, Rosowsky D, Haukaas T (2009) Seismic fragility estimates for reinforced concrete bridges subject to corrosion. Struct Saf 31(4):275–283
Christensen E, Giese A (2004) Changes in absorption spectra of nucleic acids and their derivatives following exposure to ozone and ultraviolet radiations. Arch Biochem Biophys 51:208–216
Costerton JW, Lewandowski Z, Caldwell DE, Korber DR, Lappin-Scott HM (1995) Microbial biofilms. Annu Rev Microbiol 49:711–745
Cramer SD, Covino BS, Bullard Jr, Holcomb GR, Russell JH, Nelson FJ, Laylor HM, Soltesz SM (2002) Corrosion prevention and remediation strategies for reinforced concrete coastal bridges. Cem Concr Compos 24:101–117
Derrick MR, Stulik D, Landry MJ (1999) Infrared spectroscopy in conservation science. Getty Conservation Institute, Los Angeles
Dutton MV, Evans CS (1996) Oxalate production by fungi: its role in pathogenicity and ecology in the soil environment. Can J Microbiol 42:881–895
EL-Hissy F, Khalil A, EL-Nagdy M (1990) Fungi associated with some aquatic plants collected from fresh water areas at Assiut (Upper Egypt). J Islamic Acad Sci 3:298–304
Feng Y, Komatsu S, Furukawa T, Koshiba T, Kohno Y (2008) Proteome analysis of proteins responsive to ambient and elevated ozone in rice seedlings. Agric Ecosyst Environ 125:255–265
Fischer G, Braun S, Thissen R, Dott W (2006) FT-IR spectroscopy as a tool for rapid identification and intra-species characterization of airborne filamentous fungi. J Microbiol Methods 64:63–77
Flyunt R, Theruvathu JA, Leitzke A, Von Sonntag C (2002) J Chem Soc Perkin Trans 2:1560–1572
Foucaud L, Bennasroune A, Klestadt D, Laval-Gilly P, Falla J (2006) Oxidative stress induction by short time exposure to ozone on THP-1 cells. Toxicol In Vitr 20:101–108
Gilman JC (1957) A manual of soil fungi. The Lowastate College, Ames
Goldstein JI, Newbury DE, Echlin PE, Joy DC, Romig AD, Lyman CE, Fiori C, Lifshin E (1992) Scanning electron microscopy and X-ray microanalysis. Plenum, New York
Gu J, Ford T, Berke N, Mitchell R (1998) Biodeterioration of concrete by the fungus Fusarium. Int Biodeterior Biodegradation 41:101–109
Gupta SK, Reinhart PG, Bhalla DK (2010) Enhancement of fibronectin expression in rat lung by ozone and an inflammatory stimulus. Am J Physiol Lung Cell Mol Physiol 275:330–335
Guzel-Seydim Z, Greene A, Seydim A (2004) Use of ozone in the food industry Lebenson Wiss Technol 37:453-460
Herbert F, Nadine R, Andrea B, Peggy B (2010) The effect of gaseous ozone treatment on egg components. J Sci Food Agric 90:593–598
Hosseini SM, Salari M, Jamalizadeh E, Khezripoor S, Seifi M (2010) Inhibition of mild steel corrosion in sulfuric acid by some newly synthesized organic compounds. Mater Chem Phys 119:100–105
Huth KC, Saugel B, Jakob FM, Cappello C, Quirling M, Paschos E, Ern K, Hickel R, Brand K (2007) Effect of aqueous ozone on the NF-KB system. J Dent Res 86:451–456
Ibars JR, Moreno DA, Ranninger C (1992) Microbial corrosion of stainless steel. Microbiologia 8:63–75
Ishizaki K, Shinriki N, Matsuyama H (2008) Inactivation of Bacillus spores by gaseous ozone. J Appl Microbiol 60:67–72
Jans U, Hiogne J (1998) Activated carbon and carbon black catalyzed transformation of aqueous ozone into OH–radicals. Ozone Sci Eng 20:67–90
Jozsa P, Kubmaul M, Bock E (1996) Simulation of concrete corrosion by nitrifying bacteria. Biodeterior Biodegradation 133:127–134
Juzeliunas E, Ramanauskas R, Lugauskas A, Leinartas, Samuleviciene K, Sudavicius A, Juskenas R (2007) Microbially influenced corrosion of zinc and aluminium–two-year subjection to influence of Aspergillus niger. Corros Sci 49:4098–4112
Kaltenbach H, Kiefer E, Grosskopf E, Ernst D, Sandermann H (2004) Differential transcript induction of parsley pathogenesis-related proteins and of a small heat shock protein by ozone and heat shock. Plant Mol Biol 33:343–350
Kelly MW, Robert CM (2010) Low-concentration ozone reacts with plasmalogen glycerophosphoethanolamine lipids in lung surfactant chem. Res Toxicol 23:108–117
Kern ME, Blevins KS (1997) Medical mycology a self–instructional text, 2nd edn. Davis FA CO, Philadelphia, 242 pp
Knight KL, Mudd JB (2004) The reaction of ozone with glyceraldehyde-3-phosphate dehydrogenase. Arch Biochem Biophys 229:259–269
Komanapalli IR, Lau BH (1996) Ozone-induced damage of Escherichia coli K-12. Appl Microbiol Biotechnol 46:610–614
Labjar N, Bentiss F, Chihib N, El Hajjaji S, Jama C (2010) Corrosion inhibition of carbon steel and antibacterial properties of aminotris-(methylenephosphonic) acid. Mater Chem Phys 119:330–336
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 259:680–685
Langor D, Sweeney J (2008) Ecological impacts of non-native invertebrates and fungi on terrestrial ecosystems. Biol Invasions 11:1–3
Liew CL, Prange RK (1994) Effect of ozone and storage temperature on postharvest diseases and physiology of carrots (Daucus carota L.). J Am Soc Hortic Sci 119:563–597
Little B, Staehle R, Davis R (2001) Fungal influenced corrosion of post-tensioned cables. Int Biodeterior Biodegradation 47:71–77
Mansch R, Bock E (1994) Simulation of microbiologically and chemically influenced corrosion of natural sandstone. Microbiologically influenced corrosion testing. American Society for Testing and Materials, Philadelphia, pp 203–216
Mawhinney DB, Yates JT Jr (2001) FTIR study of the oxidation of amorphous carbon by ozone at 300 K — Direct COOH formation. Carbon 39:1167–1173
Milde K, Sand W, Wolff W, Bock E (1983) Thiobacilli of the concrete walls of the Hamburg sewer system. J Gen Microbiol 129:1327–1333
Moubasher AH (1993) Soil Fungi in Qatar and other Arab Countries, 1st edn. Scientific and Applied Research Center, University of Qatar
Nagayoshi M, Fukuizum TG, Kitamura C, Yano J, Terashita M, Nishihara T (2004) Efficacy of ozone on survival and permeability of oral micro- organisms. Oral Microbiol Immunol 19:240–246
Nasar S, Munshi J (1980) Studies on the seasonal variations in the fungal population of a freshwater pond of Bhagolpur, India. Imnologica (Berlin) 12:137–139
Ortoneda M, Guarro J, Madrid M, Caracuel Z, Roncero M, Mayayo E, Pietro A (2004) Fusarium oxysporum as a multihost model for the genetic dissection of fungal virulence in plants and mammals. Infect Immun 3:1760–1766
Palli D, Sera F, Giovannelli L, Masala G, Grechi D, Bendinelli B, Caini S, Dolara P, Saieva C (2009) Environmental ozone exposure and oxidative DNA damage in adult residents of florence, Italy. Environ Pollut 157:1521–1525
Parshutin VV, Sholtoyan NS, Sidel’nikova SP, Volodina GF, Koval’ AV, Bologa OA, Shofranskii VN (2009) The influence of thiocarbohydrazide on St-3 steel corrosion in water. Surf Eng Appl Electrochem 45:212–224
Pauls KP, Thompson JE (1980) In vitro simulation of senescence-related membrane damage by ozone-induced lipid peroxidation. Nature 283:504–506
Poli G, Schaur RJ, Siems WG, Leonarduzzi G (2007) 4-Hydroxynonenal: a membrane lipid oxidation product of medicinal interest. Med Res Rev 28:569–631
Pressman S (2007) The story of ozone. Plasmafire intl, Canada, p 57
Samson RA, Reenen-Koekstra ES (1988) Introduction to food–borne fungi, 3rd edn. Published and distribushed by Centraalbureau voor Schimmelcultures, pp. 299
Sand W, Dumas T, Marcdargent S (1994) Accelerated biogenic sulfuric-acid corrosion test for evaluating the performance of calcium-aluminate based concrete in sewage applications. In: Kearns JR, Little BJ (eds) Microbiologically influenced corrosion testing. American Society for Testing and Materials, Philadelphia, pp 234–249
Segel IH (1968) Biochemical calculations, 2nd edn. Wiley, Brisbane, p 370
Shinriki N, Ishizaki K, Ikehata A, Yoshizaki T, Nomura A, Miura K, Mizuno Y (2003) Degradation of nucleic acids with ozone: II degradation of yeast RNA, yeast phenylalanine tRNA and tobacco mosaic virus RNA. Biochimica et Biophysica Acta (BBA) Nucleic Acids Protein 655:323–328
Sugita H, Asat T, Hayashi K, Mitsuya T, Amanuma K, Maruyama C, Deguchi Y (1992) Application of ozone disinfection to remove Enterococcus seriolicida, Pasteurella piscicida, and Vibrio anguillarum from seawater. Appl Environ Microbiol 58:4072–4075
Tang Y, Chevone B, Hess J (2000) Ozone-responsive proteins in a tolerant and sensitive clone of white clover (Trifolium repens). Environ Pollut 104:89–98
Theruvathu JA, Flyunt R, Aravindakumar CT, Von Sonntag C (2001) J Chem Soc Perkin Trans 2:255–269
Thomas M, Todd MW, David HG, Jane DM, Robert MC, Stephen PJ (2007) Elucidation of double bond position in unsaturated lipids by ozone electrospray lonization mass spectrometry. Anal Chem 79:5013–5022
Todar K (2009) The control of microbial growth. PhD University of Wisconsin-Madison, Department of Bacteriology
Uzarski J (2006) Investigations of bacteria viability on surfaces using functionalized alkanethiol self-assembled monolayers. MSc., Faculty of the Virginia Polytechnic Institute and State University
Valacchi G, Pagnin E, Corbacho A, Olano E, Davis P, Packer L, Cross C (2004) In vivo ozone exposure induces antioxidant/stress-related responses in murine lung and skin. Free Radic Biol Med 36:673–681
Videlal H, Herrera L (2005) Microbiologically influenced corrosion: looking to the future. Int Microbiol 8:169–180
Yamazaki T, Inoue M, Ogawa M, Shiga S, Kishimoto T, Hagiwara T, Matsumoto T, Hayashi T (2004) Inactivation of Chlamydia trachomatis and Chlamydia (Chlamydophila) pneumoniae by ozone. Lett Appl Microbiol 38:406–409
Zelinka S, Asce S, Douglas R, Rammer P, Asce M (2009) Corrosion rates of fasteners in treated wood exposed to 100% relative humidity. J Mater Civil Eng 21:758–769
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Geweely, N.S.I. Evaluation of ozone for preventing fungal influenced corrosion of reinforced concrete bridges over the River Nile, Egypt. Biodegradation 22, 243–252 (2011). https://doi.org/10.1007/s10532-010-9391-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10532-010-9391-7